Evaluation of the toxic potential of arecoline toward the third instar larvae of transgenicDrosophila melanogaster (hsp70-lacZ) Bg⁹

Abstract

Arecoline is the key component of areca nut and has been suggested as a carcinogenic agent. In the present study, the third instar larvae of transgenic were allowed to feed on a diet having 5, 10, 20, 40 and 80 μM arecoline for 24 h. After the completion of 24 h, the larvae were subjected to ONPG assay, X-gal staining, trypan blue exclusion test, oxidative stress markers, and apoptotic and comet assays. A dose-dependent increase in the β-galactosidase activity, tissue damage, glutathione-S-transferase (GST) activity, lipid peroxidation assay, monoamine oxidase (MAO), caspase-9 and 3, protein carbonyl content (PCC), apoptotic index, and DNA damage and decrease in glutathione (GSH) content, delta aminolevulinic acid dehydrogenase (δ-ALA-D), and acetylcholinesterase (AChE) activity were observed in the larvae exposed to 20, 40 and 80 μM arecoline. The results suggest that arecoline is toxic at 20, 40, and 80 μM toward the third instar larvae of transgenic . Arecoline did not show any toxic effects at 5 and 10 μM.

Exploring the neurotoxicity and changes in life cycle parameters of Drosophila melanogaster exposed to arecoline

“…But still its toxic effects on the central nervous system are warranted. In our study, it showed the toxic effects in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg 9 (Shakya & Siddique, 2018). The study performed by Shih et al (2010) on the cultured rat primary cortical neurons showed that it is potent in inducing apoptotic death by attenuating an antioxidant defence system and enhancing the oxidative stress.…”

Protective effect of the newly synthesized and characterized charge transfer (CT) complex against arecoline induced toxicity in third-instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9: experimental and theoretical mechanistic insights

“…In our present study, the ingested arecoline by the larvae may be metabolized by the cytochrome P450s present in the gut of the larvae and exhibit toxicity. 11 The supplementation of arecoline along with the newly synthesized compound CL showed a marked reduction in cytotoxicity as well as genotoxicity. It may be due to the inhibition of cytochrome P450 by CL that may prevent/reduce the metabolic activation of arecoline by cytochrome P450.…”

“…[5][6][7][8][9] We have studied the toxicity of arecoline and the protective effect of natural plant products (Geraniol) in the third instar larvae as well as adult flies of the transgenic Drosophila melanogaster (hsp70-lacZ)Bg 9 . [10][11][12] The toxic effects of arecoline could be reduced by using natural plant products and the supplementation of various vitamins. 13 The charge transfer complexes (CTCs) are self-assembled organic materials formed from the interaction between donor and p-acceptor moieties through N + -HÁ Á ÁO À bonding, and this concept was first introduced by Mulliken and Foster.…”

“…5–9 We have studied the toxicity of arecoline and the protective effect of natural plant products (Geraniol) in the third instar larvae as well as adult flies of the transgenic Drosophila melanogaster (hsp70-lacZ)Bg 9 . 10–12 The toxic effects of arecoline could be reduced by using natural plant products and the supplementation of various vitamins. 13…”

Effect of Majun Baladur on life span, climbing ability, oxidative stress and dopaminergic neurons in the transgenic Drosophila model of Parkinson's disease

“…As there is no permanent cure for the disease and the efficacy of the synthetic drugs also decline after some period of the treatment [10], the current research is now directed towards finding naturally occurring antioxidants of plant origin [11]. In this context, the unani system of traditional medicine is widely accepted in Indian sub continent and is practiced in India [12]. Majun Baladur (MB) is a polyherbal formulation, prescribed to patients having neurodegenerative ailments, under unani system of traditional medicine.…”