Evaluation of the total content of lipid-soluble antioxidants in blood plasma samples employing a simple chemiluminescence quenching procedure

Escobar, Jorge; Cardenas, Gabriela Moran; Lissi, Eduardo

doi:10.1016/s0165-022x(97)01215-3

Cited by 3 publications

(1 citation statement)

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“…The relation between I 0 / I is related to the concentration of lipid‐soluble antioxidants. A standard curve was established with a set of serial dilutions of α‐tocopherol (Escobar et al. 1997).…”

Section: Methodsmentioning

confidence: 99%

Brain antioxidant status in a high pressure–induced rat model of glaucoma

Ferreira

Lerner²,

Reides

et al. 2012

Acta Ophthalmologica

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ABSTRACT.Purpose: The goal of the present study is to establish the antioxidant status in the brain of a high pressure-induced rat model. Methods: Ocular hypertension was induced in rats (n = 12) cauterizing two episcleral veins under a surgical microscope. A sham procedure (n = 12) was performed in the control group. The markers evaluated in the brain 7 days after surgery were as follows: spontaneous chemiluminescence, protein carbonylation, nitrite concentration, total reactive antioxidant potential (TRAP), ascorbic acid, glutathione, vitamin E and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase. Results: Chemiluminescence in glaucoma was 55% higher than in controls (393 ± 20 cpm ⁄ mg protein, p < 0.001). Protein carbonylation in glaucoma was 93% higher than in controls (1.15 ± 0.18 nmol ⁄ mg protein, p < 0.001). Nitrite concentration was 5.30 ± 0.25 lM for glaucoma (controls 4.41 ± 0.24 lM, p < 0.05). Total reactive antioxidant potential decreased by 42% in glaucoma (controls 153 ± 14 lM Trolox, p < 0.001). Ascorbic acid was 67 ± 26 lM for glaucoma (controls 275 ± 22 lM, p < 0.001). Vitamin E was 0.58 ± 0.05 lmol ⁄ g organ for glaucoma (controls 1.10 ± 0.06 lmol ⁄ g organ, p < 0.01). Glutathione was 1.98 ± 0.13 lmol ⁄ g organ for glaucoma (controls 8.19 ± 0.71 lmol ⁄ g organ, p < 0.001). Superoxide dismutase and GPx were increased in glaucoma by 42 and 59%, respectively (p < 0.05). Conclusions: Reactive oxygen and nitrogen species were increased in glaucoma, the increase in chemiluminescence, protein carbonylation and nitrite levels could be evidenced by this situation. The decrease in nonenzymatic antioxidants and a compensatory increase in SOD and GPx activity may have been a consequence of an increase in oxidative processes.

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Section: Methodsmentioning

confidence: 99%