Evaluation of the time, labor, and money required for manual and automated nucleic acid (RNA) isolation for the detection of SARS-COV-2 by QRT-PCR using the qiamp viral RNA mini kit and kingfisher flex
Chetana Roat,
Nilay Harshadkumar Dave
Abstract:We undertook this cross-sectional investigation to assess the time, manpower, and average run cost per sample using manual Qiamp Viral RNA micro kit (Qiagen) and automated kingfisher flex instrument extraction methods for SARS-Cov-2 identification.The study used 120 Viral Transport Media-collected nasopharyngeal/ oropharyngeal swabs.Magnetic bead-based RNA extraction was performed using the Thermo Fisher Scientific kingfisher flex instrument and manual Extraction was Silica membrane-based Qiagen spin column ki… Show more
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