Evaluation of the TbgI<sub>2</sub> and TbgI<sub>17</sub> Tandem Repeat Antigens as Potential Antigens for the Diagnosis of <i>Trypanosoma brucei rhodesiense</i>

Abstract

Human African trypanosomiasis (HAT) affects up to half a million people every year in sub-Saharan Africa. Interruption of transmission of the disease by early diagnosis and treatment is core to the control and eventual elimination of HAT. The routine diagnostic method for HAT is light microscopy of blood samples. The present study sought to evaluate the potential of TbgI 2 and TbgI 17 tandem repeat antigens as candidates for the diagnosis of Trypanosoma brucei rhodesiense. The expressed proteins were purified and the antigenic reactivity evaluation was done using multiplex assay using sera obtained from HAT patients. Receiver operating characteristic analysis showed that recombinant antigen, TbgI 2 had high sensitivity for sera from patients infected with T. b. rhodesiense with the area under the curve being 0.577 and a sensitivity of 0.641 and specificity 0.650. The results suggest that TbgI 2 is a potential biomarker for T. b. rhodesiense HAT serodiagnostic tests.

Novel protein candidates for serodiagnosis of African animal trypanosomosis: Evaluation of the diagnostic potential of lysophospholipase and glycerol kinase from Trypanosoma brucei

“…Several studies have shown that the main limitation of single protein based assays is often lack of sensitivity [19,22,25,26]. As expected, this pattern of response is equally present for the two tested proteins.…”

Use of Polyclonal Antibody for the Diagnosis of Human African Trypanosomiasis

“…The concentrations of Tbg I2 and Tbg I17 expressed proteins were 0.345 and 0.221 mg/mL, respectively presented in the Table 1. The study conducted by Irumva et al, 2019 reported a concentration for Tbg I2 and Tbg I17 expressed proteins of 1.299 mg/mL and 0.517 mg/mL, respectively [13]. These differences can be related to the short time given for the culture to grow in our study, whereas a long time was given for the culture to grow in Irumva et al's 2019 study.…”

Development of an immunoassay for the detection of human IgG against hepatitis C virus proteins using magnetic beads and flow cytometry

“…Unlike other immunoassays such as ELISA and immunoblot, immunoassays using flow cytometers apply lasers that recognize only the soluble particles marked and of pre-established size. Recent studies have demonstrated its potential use in new applications [22,23]. This methodology presents a minimum interference of unmarked particles, thus allowing reduction of the washing steps, handling and testing time [24,25].…”