Evaluation of the Tasso-SST® capillary blood microsampling device for the measurement of endogenous uracil levels

Dewes, Milene Menestrina; Silva, Laura Cé da; Meireles, Yasmin Fazenda; Freitas, Mariana Viana de; Bastiani, Marcos Frank; Lizot, Lilian Feltraco; Hahn, Roberta Zilles; Antunes, Marina Venzon; Linden, Rafael

doi:10.1016/j.clinbiochem.2022.06.003

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…The widely evaluated Tasso-SST device collects ~300 µL whole blood [1,[8][9][10][11] into a tube that contains a clot activator and separation gel but is no longer available [12]. To date, these devices have been assessed for clinical chemistry [3,4,[13][14][15], haematology [2], and serology [8][9][10][11][16][17][18], with overall good analytical performance and participant acceptance. It must be noted, however, that most automated analysers require a dead volume (200-300 µL) of specimen in addition to the volume required for analyte testing.…”

Section: Introductionmentioning

confidence: 99%

Painless Capillary Blood Collection: A Rapid Evaluation of the Onflow Device

Noble,

Dixon,

Moran

et al. 2023

Diagnostics

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Blood-based diagnostics are critical for many medical decisions, but mostly rely on venepuncture, which can be inconvenient and painful. The Onflow Serum Gel (Loop Medical SA, Vaud, Lausanne, Switzerland) is a novel blood collection device that utilises needle-free technology to collect capillary blood. In this pilot study, 100 healthy participants were enrolled and provided two Onflow collected specimens and one venous blood specimen. Five chemistry analytes (AST, ALT, LDH, potassium, creatinine) and haemolysis were measured per specimen, and laboratory analyte results were compared. Onflow was found to be more acceptable than venepuncture with lower pain ratings, and 96.5% of participants would use the Onflow method again. All phlebotomists (100%) found Onflow intuitive and user-friendly, with ~1 mL of Onflow blood successfully collected from 99% of participants in <12 min (mean: 6 min, 40 s) and 91% collected on the first attempt. ALT and AST analytes showed no difference in performance, while creatinine generated a negative bias (−5.6 µmol/L), and increased variability was noted with potassium (3.6%CV) and LDH (6.7%CV), although none were clinically relevant. These differences may be due to 35% of Onflow collected specimens having “mild” haemolysis. Onflow is a promising alternative blood collection device that should now be evaluated in participants with expected abnormal chemistries and as an option for self-collection.

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Section: Introductionmentioning

confidence: 99%

Painless Capillary Blood Collection: A Rapid Evaluation of the Onflow Device

Noble,

Dixon,

Moran

et al. 2023

Diagnostics

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“…Microsampling technologies have been evaluated in biomarker research studies for the diagnosis and prognosis of various conditions and the prediction and monitoring of response to an intervention 24,41,42,132–144 . Omics‐based approaches, such as metabolomics and proteomics, are increasingly employed for the generation of large amounts of data to facilitate the rapid discovery of biomarkers.…”

Section: Applicationsmentioning

confidence: 99%

“…However, at‐home sampling using TASSO‐SST for uracil measurements is not feasible due to the compound's high instability. Nevertheless, microsampling technology can be implemented at the hospital to reduce the burden of repeated blood draws in cancer patients 137 . A randomised, control trial evaluated the accuracy, feasibility and acceptability of microsampling techniques in rheumatoid arthritis patients by measuring inflammation markers and autoantibodies in three matrices: conventional venous blood, upper arm capillary blood obtained using TASSO‐SST and finger prick capillary blood.…”

Section: Applicationsmentioning

confidence: 99%

“…Microsampling technologies have been evaluated in biomarker research studies for the diagnosis and prognosis of various conditions and the prediction and monitoring of response to an intervention. 24 , 41 , 42 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 Omics‐based approaches, such as metabolomics and proteomics, are increasingly employed for the generation of large amounts of data to facilitate the rapid discovery of biomarkers. A study assessed the DBS and DPS technology for non‐targeted lipidomic analysis as high throughput profiling and quantification of lipids could expand knowledge of underlying disease pathologies.…”

Section: Applicationsmentioning

confidence: 99%

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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With the development of highly sensitive bioanalytical techniques, the volume of samples necessary for accurate analysis has reduced. Microsampling, the process of obtaining small amounts of blood, has thus gained popularity as it offers minimal‐invasiveness, reduced logistical costs and biohazard risks while simultaneously showing increased sample stability and a potential for the decentralization of the approach and at‐home self‐sampling. Although the benefits of microsampling have been recognised, its adoption in clinical practice has been slow. Several microsampling technologies and devices are currently available and employed in research studies for various biomedical applications. This review provides an overview of the state‐of‐the‐art in microsampling technology with a focus on the latest developments and advancements in the field of microsampling. Research published in the year 2022, including studies (i) developing strategies for the quantitation of analytes in microsamples and (ii) bridging and comparing the interchangeability between matrices and choice of technology for a given application, is reviewed to assess the advantages, challenges and limitations of the current state of microsampling. Successful implementation of microsampling in routine clinical care requires continued efforts for standardization and harmonization. Microsampling has been shown to facilitate data‐rich studies and a patient‐centric approach to healthcare and is foreseen to play a central role in the future digital revolution of healthcare through continuous monitoring to improve the quality of life.

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Capillary blood self‐collection for high‐throughput proteomics

El‐Sabawi,

Huang,

Tanriverdi

et al. 2024

Proteomics

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In this study, we sought to compare protein concentrations obtained from a high‐throughput proteomics platform (Olink) on samples collected using capillary blood self‐collection (with the Tasso+ device) versus standard venipuncture (control). Blood collection was performed on 20 volunteers, including one sample obtained via venipuncture and two via capillary blood using the Tasso+ device. Tasso+ samples were stored at 2°C–8°C for 24‐hs (Tasso‐24) or 48‐h (Tasso‐48) prior to processing to simulate shipping times from a study participant's home. Proteomics were analyzed using Olink (384 Inflammatory Panel). Tasso+ blood collection was successful in 37/40 attempts. Of 230 proteins included in our analysis, Pearson correlations (r) and mean coefficient of variation (CV) between Tasso‐24 or Tasso‐48 versus venipuncture were variable. In the Tasso‐24 analysis, 34 proteins (14.8%) had both a correlation r > 0.5 and CV < 0.20. In the Tasso‐48 analysis, 68 proteins (29.6%) had a correlation r > 0.5 and CV < 0.20. Combining the Tasso‐24 and Tasso‐48 analyses, 26 (11.3%) proteins met these thresholds. We concluded that protein concentrations from Tasso+ samples processed 24–48 h after collection demonstrated wide technical variability and variable correlation with a venipuncture gold‐standard. Use of home capillary blood self‐collection for large‐scale proteomics should be limited to select proteins with good agreement with venipuncture.

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Evaluation of the Tasso-SST® capillary blood microsampling device for the measurement of endogenous uracil levels

Cited by 5 publications

References 14 publications

Painless Capillary Blood Collection: A Rapid Evaluation of the Onflow Device

Painless Capillary Blood Collection: A Rapid Evaluation of the Onflow Device

Blood microsampling technologies: Innovations and applications in 2022

Capillary blood self‐collection for high‐throughput proteomics

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