Evaluation of the strains of Acinetobacter and Enterobacter as potential biocontrol agents against Ralstonia wilt of tomato

Xue, Qing-Yun; Chen, Yu; Li, Shimo; Chen, Lifeng; Ding, Guo-Chun; Guo, Dawei; Guo, Jianhua

doi:10.1016/j.biocontrol.2008.11.004

Cited by 143 publications

(80 citation statements)

References 20 publications

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“…Ralstonia solanacearum and Bacillus spp. were routinely cultured on yeast extract peptone glucose agar (YPGA) (Xue et al, 2009) and Luria bertani agar (LBA), respectively. Tomato seeds (cv.…”

Section: Origin Of Bacterial Strains Plant Materials and Chemical Inmentioning

confidence: 99%

Synergistic effect of acetyl salicylic acid and DL-Beta-aminobutyric acid on biocontrol efficacy of Bacillus strains against tomato bacterial wilt

Almoneafy¹,

Ojaghian²,

Seng-fu³

et al. 2013

Trop. plant pathol.

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This study was conducted to assess the biocontrol efficacy of Bacillus subtilis (strain 4812) and Bacillus methylotrophicus (strain H8) individually or in combination with two plant defense inducers including Acetyl salicylic acid (ASA) and DL-Beta-aminobutyric acid (BABA) against tomato wilt caused by Ralstonia solanacearum. The pathogen was significantly inhibited by all treatments in the greenhouse and in vitro tests. The treatments H8+ASA and 4812+H8 were found to be the most effective treatments in in vitro tests. Applied on tomato seeds and as soil drenching, the disease was most inhibited by H8 whereas 4812+H8+ASA was the least effective treatment. High activity of phenylalanine ammonia-lyase was observed in the plants treated with 4812+H8, H8+ASA and 4812+H8+ASA. The highest expression of peroxidase and polyphenoloxidase enzymes was found in the plants treated with H8, 4812+H8 and H8+ASA. The result of real time PCR showed that concentration of the pathogen in stem tissues was significantly reduced in all treated plants and H8+ASA was the most effective treatment. This study revealed that combined application of Bacillus strains can be considered as a more effective biocontrol agent against tomato bacterial wilt. Furthermore, the synergistic effect of plant defense inducers was variable to different Bacillus strains.

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Section: Origin Of Bacterial Strains Plant Materials and Chemical Inmentioning

confidence: 99%

Synergistic effect of acetyl salicylic acid and DL-Beta-aminobutyric acid on biocontrol efficacy of Bacillus strains against tomato bacterial wilt

Almoneafy¹,

Ojaghian²,

Seng-fu³

et al. 2013

Trop. plant pathol.

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“…A detecção de antibiose "in vitro" é um método que tem sido usado para seleção inicial de rizobactérias como possíveis agentes de biocontrole contra R. solanacearum (Moura & Romeiro, 1999;Lemessa & Zeller, 2007;Messiha et al, 2007.;Xue et al, 2009). No presente estudo, todas as rizobactérias produziram zona de inibição "in vitro" de R. solanacearum, portanto estes biocontroladores podem estar atuando por antibiose.…”

Section: Discussionunclassified

Controle biológico da murcha do tomateiro causada por Ralstonia solanacearum e Fusarium oxysporum f. sp. lycopersici por rizobactérias

Rocha¹,

Moura²

2013

Trop. plant pathol.

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RESUMOA busca por alternativas ao uso intensivo de agrotóxicos no controle de doenças tem recebido grande atenção da pesquisa agrícola. Rizobactérias têm reconhecida capacidade de reduzir doenças em diversas culturas e promover de crescimento de plantas. O objetivo deste trabalho foi avaliar a eficácia de seis isolados de rizobactérias, pré-selecionadas, no controle de Ralstonia solanacearum e Fusarium oxysporum f. sp. lycopersici (FOL), em casa-de-vegetação e relacionar este comportamento a produção de compostos "in vitro". Foi avaliada a capacidade de estas rizobactérias produzirem quitinases, amilases, lipases, compostos antibióticos e de solubilizar fosfato. A microbiolização das sementes com a rizobactéria DFs1421 (Pseudomonas sp.) reduziu os valores de AACPD de murcha bacteriana em ambos os ensaios (36,6 e 91,7% no primeiro e segundo ensaios respectivamente). Este controle pode ser associado à produção de compostos responsáveis pela antibiose observada "in vitro". Isolados de Streptomyces (DFs1296 e DFs1315), bem como de Bacillus (DFs1414) e o indutor químico ASM reduziram significativamente a murcha de fusário, variando de 22,5 a 76%. O controle observado por parte das rizobactérias pode ser atribuído à atividade quitinolítica e/ou antibiótica por compostos voláteis. Palavras-chaves: Solanum lycopersicum, biocontrole, murcha bacteriana, murcha de fusário ABSTRACT Biological control of tomato wilt caused by Ralstonia solanacearum and Fusarium oxysporum f. sp. lycopersici by rhizobacteriaReplacing the intensive use of pesticides for alternative methods in disease control has been an important aim for agricultural research. Rhizobacteria are known to be capable of reducing diseases levels in many crops and also of promoting plant growth. The objective of this work was to evaluate the efficacy of six pre-selected rhizobacteria isolates in controlling Ralstonia solanacearum and Fusarium oxysporum f. sp. lycopersici (FOL) under controlled conditions in a greenhouse, and to link such capacity to the "in vitro" production of selected biologically active compounds. The ability of these rhizobacteria isolates to produce chitinases, amylases, lipases, antibiotic compounds was investigated. Additionally, their ability in solubilizing phosphate was also checked. Microbiolization of seeds with one rhizobacterium DFs1421 (Pseudomonas sp.) reduced the tomato wilt AUDPC in both assays (36.6 and 91.7% in the first and second assays respectively). Such efficacy in wilt control is likely to be linked with the production of antibiotics as observed "in vitro". Streptomyces (DFs1296 and DFs1315) and Bacillus (DFs1414), and the chemical inducer (ASM) reduced significantly fusarium wilt ranging 22.5 to 76%. This may be owing to the observed chitinolytic activity and / or antibiosis in the presence of volatile compounds.

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“…They play an important role for their effects on plants including beneficial associations in the rhizosphere, and also represent a valuable resource in the search for secondary metabolites with useful therapeutic applications [2,3]. Within our screening program for isolation of microorganisms containing bioactive components [4], astrain of Aspergillus terreus was isolated from the rhizosphere soil of tomato. The title compound, dihydrocitrinone, was first described by [5], and elucidated on the basic of spectroscopic analysis [6,7].…”

Section: Discussionmentioning

confidence: 99%

Crystal structure of (3R,4S)-6,8-dihydroxy-3,4,5-trimethyl-1- oxoisochroman-7-carboxylic acid, C13H14O6

Chang¹,

Li²,

Fu³

et al. 2009

Zeitschrift Für Kristallographie - New Crystal Structures

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Evaluation of the strains of Acinetobacter and Enterobacter as potential biocontrol agents against Ralstonia wilt of tomato

Cited by 143 publications

References 20 publications

Synergistic effect of acetyl salicylic acid and DL-Beta-aminobutyric acid on biocontrol efficacy of Bacillus strains against tomato bacterial wilt

Synergistic effect of acetyl salicylic acid and DL-Beta-aminobutyric acid on biocontrol efficacy of Bacillus strains against tomato bacterial wilt

Controle biológico da murcha do tomateiro causada por Ralstonia solanacearum e Fusarium oxysporum f. sp. lycopersici por rizobactérias

Crystal structure of (3R,4S)-6,8-dihydroxy-3,4,5-trimethyl-1- oxoisochroman-7-carboxylic acid, C13H14O6

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