Evaluation of the STR typing kit PowerPlex™ 16 with respect to technical performance and population genetics: a multicenter study

Henke, Lotte; Aaspõllu, Anu; Biondo, R.; Budowle, Bruce; Drobnič, Katja; Eede, P.H. van; Felske-Zech, Heike; Simón, Lourdes Fernández de; Garafano, L.; Gehrig, Christian; Luckenbach, C.; Malik, Naseem; Muche, M.; Parson, Walther; Primorac, Dragan; Schneider, P.M.; Thomson, J. Arthur; Vaněk, Daniel

doi:10.1016/s0531-5131(02)00342-4

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…Here, f(a 11 a 12 ) denotes the population frequency of genotype a 11 a 12 , which can be calculated from known allele frequency estimates (http://www.uni-duesseldorf.de/WWW/MedFak/ Serology/) [1,2] because the population of interest is assumed to be at HWE anyway.…”

Section: Methodsmentioning

confidence: 99%

Potentials and limits of pairwise kinship analysis using autosomal short tandem repeat loci

2010

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At least in principle, most instances of complex kinship testing can be reduced to pairwise kinship cases involving two critical family members that either link or separate presumed sub-branches of a family. In the European population, the 34 short tandem repeats (STRs) currently used in forensic genetics are sufficiently powerful to allow assessment of disputed first and second but not lower degrees of pairwise blood relatedness. We provide estimates of the means and variances of marker-specific log-likelihood ratios, using large-sample approximation and assuming different scenarios of pairwise kinship analysis. These estimates allow power calculations to be performed for any combination of the available STRs. Since some of the markers considered are physically linked, chromosome-wide likelihood calculations in kinship cases other than parent-child duos (and trios) have to take the reduced rates of meiotic inter-marker recombination into account. We show by simulation that this requirement may be ignored when discriminating distant hypotheses about kinship, but that linkage may play an important role in the biostatistical analysis of more intricate cases.

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Section: Methodsmentioning

confidence: 99%

Potentials and limits of pairwise kinship analysis using autosomal short tandem repeat loci

2010

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“…It is widely accepted that general databases are usually adequate when studying autosomal STRs, as they show little variation among different population groups of the same ethnic background (e.g., Caucasian) [6,12]. However, sex chromosomes may be more prone to show population differences.…”

Section: Discussionmentioning

confidence: 99%

Forensic efficiency of microsatellites and single nucleotide polymorphisms on the X chromosome

2007

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Polymorphisms located on the X chromosome are being increasingly used in forensic studies. However, they have not been studied as extensively as the autosomal and Y-linked polymorphisms. Therefore, we planned to study the population genetics of two sets of X-linked markers, including short-tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), and particularly analyze the differences at the microgeographical level. Six X-linked STRs (DXS 9895, DXS 7132, DXS 9898, DXS 6789, GATA 172D05, and DXS 7130) and ten SNPs (rs1229078, rs1544545, rs4442270, rs1874111, rs5968332, rs1166756, rs12849634, rs5932595, rs203648, and rs611711) were studied in two population samples from Cantabria, northern Spain, a mixed coastal population and a relatively isolated small population in the Pas valley. There were statistically significant differences in allelic frequencies of the six STRs studied between both populations. On the other hand, only one out of ten SNPs studied showed between-population differences. Overall Fst values were 0.4-2.9% and 1.8-2.2% for the STRs and the SNPs, respectively. The overall power of discrimination for female samples was higher than 99.99% for both groups of markers. Therefore, these sets of X-linked STRs and SNPs seem to be potentially useful in forensic genetics, but care should be taken when interpreting results from cases that originate from small and relatively isolated populations.

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“… AmpF/STR‐ SGM Plus (Applied Biosystems, Weiterstadt, Germany) (1), PowerPlex 16 (Promega, Mannheim, Germany) (1,6), FFFL (Promega) (1), AmpF/STR Profiler Plus ID (Applied Biosystems) (1), Power Plex ES (Promega) (1), genres MPX‐3SE (Serac, Bad Hamburg, Germany), and Humantype Chimera (Biotype, Dresden, Germany). …”

Section: — Maternal and Paternal Mutation Rates Observed At 33 Autosomentioning

confidence: 99%

Supplemented Data on Mutation Rates in 33 Autosomal Short Tandem Repeat Polymorphisms

Henke

2006

Journal of Forensic Sciences

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Evaluation of the STR typing kit PowerPlex™ 16 with respect to technical performance and population genetics: a multicenter study

Cited by 4 publications

References 5 publications

Potentials and limits of pairwise kinship analysis using autosomal short tandem repeat loci

Potentials and limits of pairwise kinship analysis using autosomal short tandem repeat loci

Forensic efficiency of microsatellites and single nucleotide polymorphisms on the X chromosome

Supplemented Data on Mutation Rates in 33 Autosomal Short Tandem Repeat Polymorphisms

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