Evaluation of the simulated physiological oocyte maturation system for improving bovine in vitro embryo production

Guimarães, A. L. S.; Pereira, Sidney Alcântara; Leme, L. O.; Dode, M. A. N.

doi:10.1016/j.theriogenology.2014.07.042

Cited by 39 publications

(14 citation statements)

References 28 publications

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“…Lower in vitro development of oocytes derived from prepubertal donors employing 6–7 and 7–10 months old stimulated donors compared with adult donors has been previously reported [ 13 , 14 ], whereas other studies using 1–4 months old unstimulated donors found similar cleavage and blastocyst rates as for adult donors [ 22 ]. Variable developmental rates have been reported using the SPOM system [ 27 , 38 ], and the high success rates in the first report could not yet be confirmed, possibly due to methodological changes in culture conditions [ 39 ]. In our approach, prepubertal and adult oocytes produced similar blastocyst rates and the increased cAMP levels did not further improve in vitro developmental rates both in prepubertal and in adult oocytes.…”

Section: Discussionmentioning

confidence: 99%

Cyclic AMP Affects Oocyte Maturation and Embryo Development in Prepubertal and Adult Cattle

et al. 2016

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High cAMP levels during in vitro maturation (IVM) have been related to improved blastocyst yields. Here, we employed the cAMP/cGMP modulators, forskolin, IBMX, and cilostamide, during IVM to unravel the role of high cAMP in early embryonic development produced from prepubertal and adult bovine oocytes. Oocytes were collected via transvaginal aspiration and randomly assigned to three experimental groups: TCM24 (24h IVM/control), cAMP30 (2h pre-IVM (forskolin-IBMX), 30h IVM-cilostamide), and DMSO30 (Dimethyl Sulfoxide/vehicle control). After IVM, oocytes were fertilized in vitro and zygotes were cultured in vitro to blastocysts. Meiotic progression, cAMP levels, mRNA abundance of selected genes and DNA methylation were evaluated in oocytes. Blastocysts were used for gene expression or DNA methylation analyses. Blastocysts from the cAMP30 groups were transferred to recipients. The cAMP elevation delayed meiotic progression, but developmental rates were not increased. In immature oocytes, mRNA abundance of PRKACA was higher for cAMP30 protocol and no differences were found for PDE3A, SMAD2, ZAR1, PRDX1 and SLC2A8. EGR1 gene was up-regulated in prepubertal cAMP30 immature oocytes and down-regulated in blastocysts from all in vitro treatments. A similar gene expression profile was observed for DNMT3b, BCL2L1, PRDX1 and SLC2A8 in blastocysts. Satellite DNA methylation profiles were different between prepubertal and adult oocytes and blastocysts derived from the TCM24 and DMSO30 groups. Blastocysts obtained from prepubertal and adult oocytes in the cAMP30 treatment displayed normal methylation profiles and produced offspring. These data indicate that cAMP regulates IVM in prepubertal and adult oocytes in a similar manner, with impact on the establishment of epigenetic marks and acquisition of full developmental competency.

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Section: Discussionmentioning

confidence: 99%

Cyclic AMP Affects Oocyte Maturation and Embryo Development in Prepubertal and Adult Cattle

et al. 2016

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“…This method combines the meiotic inhibition for 12 h by drugs increasing the oocyte cAMP level and stimulation of maturation by FSH, resulting in slower maturation (6 h longer) and increased development rate and quality after IVF. Even if this methodology is still controversial (Guimarães et al, 2015), it represents a promising direction for further research.…”

Section: Oocyte Maturationmentioning

confidence: 99%

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Ferré¹,

Kjelland²,

Strøbech³

et al. 2020

Animal

134

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In vitro production (IVP) of embryos and associated technologies in cattle have shown significant progress in recent years, in part driven by a better understanding of the full potential of these tools by end users. The combination of IVP with sexed semen (SS) and genomic selection (GS) is being successfully and widely used in North America, South America and Europe. The main advantages offered by these technologies include a higher number of embryos and pregnancies per unit of time, and a wider range of potential female donors from which to retrieve oocytes (including open cyclic females and ones up to 3 months pregnant), including high index genomic calves, a reduced number of sperm required to produce embryos and increased chances of obtaining the desired sex of offspring. However, there are still unresolved aspects of IVP of embryos that limit a wider implementation of the technology, including potentially reduced fertility from the use of SS, reduced oocyte quality after in vitro oocyte maturation and lower embryo cryotolerance, resulting in reduced pregnancy rates compared to in vivo–produced embryos. Nevertheless, promising research results have been reported, and work is in progress to address current deficiencies. The combination of GS, IVP and SS has proven successful in the commercial field in several countries assisting practitioners and cattle producers to improve reproductive performance, efficiency and genetic gain.

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“…Furthermore, the numbers of oocytes per treatment group were based on a previous report (300–500 oocyte per treatment group) (Guimarães et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…Droplets containing oocytes were placed on 35 mm plastic dishes, covered with mineral oil, and then incubated at 38.5°C under 5% CO 2 in air and maximum humidity for a period of 24 h (Shamsuddin & Rodriguez-Martinez 1994). Furthermore, the numbers of oocytes per treatment group were based on a previous report (300-500 oocyte per treatment group) (Guimarães et al 2015).…”

Section: In Vitro Maturation (Ivm) Of Oocytesmentioning

confidence: 99%

Effects of supplementation of iodixanol to semen extender on quality and fertilization ability of frozen–thawed Thai native bull sperm

Chuawongboon

Sirisathien

Pongpeng

et al. 2017

Animal Science Journal

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This study investigates the effects of iodixanol supplementation in varied concentrations to Tris egg yolk (TEY) extender on the quality and fertilization ability of frozen-thawed sperm of Thai native bulls. Each ejaculate was divided into four different groups, as follows: sperm were treated with TEY extender (control group) and TEY extender supplemented with three different concentrations of iodixanol (1.25%, 2.50% and 5.00%). Semen straws were frozen in liquid nitrogen vapor. After thawing, sperm motility characteristics, viability, plasma membrane integrity and acrosome integrity were determined. Also, frozen-thawed spermatozoa from all groups were used for in vitro fertilization and artificial insemination (AI) in natural estrus Thai native cows. The results showed that the post-thaw quality of the 2.50% iodixanol group was superior to the other iodixanol groups (P < 0.05). However, iodixanol had no beneficial effect on post-thaw sperm in vitro fertilization ability and pregnancy rate after AI (P > 0.05). It can be concluded that the supplementation of 2.50% iodixanol extender significantly improves the progressive motility, viability, plasma membrane integrity and acrosome integrity of cryopreserved semen from Thai native bulls, but it has no beneficial effect on in vitro fertilization ability and pregnancy rate after AI.

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Evaluation of the simulated physiological oocyte maturation system for improving bovine in vitro embryo production

Cited by 39 publications

References 28 publications

Cyclic AMP Affects Oocyte Maturation and Embryo Development in Prepubertal and Adult Cattle

Cyclic AMP Affects Oocyte Maturation and Embryo Development in Prepubertal and Adult Cattle

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Effects of supplementation of iodixanol to semen extender on quality and fertilization ability of frozen–thawed Thai native bull sperm

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