Evaluation of the Side Effects of Different Doses of Spirulina on Various Organs in Rats: Biochemical, Histological and Histochemical Assessment

Abstract

Background: Spirulina is a biomass of cyanobacteria (blue-green algae) that can be consumed by humans and other animals. It is a type of microscopic algae in the shape of a perfect spiral coil. It is one of the many dietary supplements commercially available. It contains an abundant amount of essential amino acids, fatty acids, protein, vitamins, minerals, and pigments. Spirulina has many health benefits in preventing or managing hypercholesterolemia, hyperglycemia, cardiovascular diseases, diabetes, and other metabolic disease. Aim: The principal objective of this study was to estimate the possible side effects of two doses (500 and 1000 mg/kg b. wt) of Spirulina supplementation on as the liver, kidney and testis of rats. Materials and Methods: Adult male albino rats (Rattus norvegicus) weighing 120-140g were divided into three groups, G1: control; G2: treated with Spirulina at 500 mg/kg b. wt; and G3: treated with Spirulina at 1000 mg/kg b. wt. The administration was undertaken by gastric tube for 21 days. Biochemical analysis was done for measuring the blood levels of ALT, AST, urea, and creatinine. The liver, kidney and testis were excised, sectioned, and stained with different histological and histochemical stains for histopathological studies. Results: Spirulina induced many histological changes in the hepatic, renal, and testicular tissues, as well as biochemical changes in a dose-dependent manner. Conclusion: Uptake of uncontrolled supplementary doses of Spirulina may induce biochemical dysfunction and histopathological changes in some vital organs. Therefore, caution must be taken at using Spirulina as a food supplement. In fact, further biochemical and histopathological studies on its effect on other vital organs are needed for optimal dose-finding that should be considered for both efficacy and toxicity of Spirulina on the human body.

Effect of Spirulina on Cochlea Histopathological Changes in Wistar Rats Induced by Kanamicin

“…and also block free radicals (5). The reported side effects in humans are insomnia and gastric problems, the cause of which is still uncertain (14) in people who consume 1g of Spirulina per day side effects include facial flushing, headache, stomach pain, sweating, and muscle aches (17), whereas in mice there were no negative side effects other than weight gain (18) and several studies are still assessing the effects of spirulina on the liver, kidneys and testes of rats (17). Antioxidants are compounds that donate electrons or electron donors that are able to inactivate the development of an oxidation reaction, by preventing the formation of ROS (5).…”