Evaluation of the Selected 12-locus MIRU for Genotyping Beijing Family Mycobacterium tuberculosis in Korea

Kang, Heeyoon; Ryoo, Sungweon; Park, Young-Kil; Lew, Woo Jin

doi:10.4046/trd.2009.67.6.499

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…The proportion of Beijing genotypes in our study was not as high as in other Asian countries: 58% in Thailand, 69% in Hong Kong, 77% in Korea, 80% in Japan, and 82% in China [11] , [12] , [13] , [14] , [15] . In a previous study based on samples from a nation-wide survey on drug resistant TB in Vietnam in 2005, the overall proportion of Beijing isolates was 35% (30% in the North, 28% in the Center and 39% in the South) [16] .…”

Section: Discussioncontrasting

confidence: 81%

High Prevalence of Beijing and EAI4-VNM Genotypes among M. tuberculosis Isolates in Northern Vietnam: Sampling Effect, Rural and Urban Disparities

et al. 2012

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A total of 221 isolates of M. tuberculosis were sampled from hospitals and the general population in the northern plain of Vietnam, one of the most populated region of the country. Genotypic composition and diversity were characterized, and we investigated how they are affected by sampling (hospital vs. general population), correcting for potential confounding effects (location, age and gender of the patients). Spoligotyping and 12 MIRU-VNTR typing were used as first line. Then 15 MIRU-VNTR standard set was used, making 21 MIRU-VNTR typing for the clustered isolates. Result showed that 8 lineages and 13 sub-lineages were circulating in the region. The most predominant lineages were Beijing (38.5%) and EAI (38.5%). Others appeared with small proportions H (1.4%), LAM (1.8%), T (8.1%), X (0.9%), MANU (2.3%), and Zero (0.4%). Higher clustering rate was found in the hospital samples (17.9% in urban and 19.2% in rural areas) compared to the population ones (0%). The typical Vietnamese EAI4-VNM sub-lineage of EAI lineage accounted for 67% of EAI strains and was associated with older ages. Beijing genotypes were associated with younger, urban population and were characterized by high clustering rates. These characteristics strongly suggest that Beijing strains are invading the population, replacing the local EAI-VNM4, thus predicting a more serious tuberculosis situation in the future in the absence of more effective control strategies.

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Section: Discussioncontrasting

confidence: 81%

High Prevalence of Beijing and EAI4-VNM Genotypes among M. tuberculosis Isolates in Northern Vietnam: Sampling Effect, Rural and Urban Disparities

et al. 2012

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“…The MIRU-VNTR 12 loci method is quick and easy, and it has been evaluated in several studies in different locations (Kang et al , 2009). Because it has a discriminatory power equivalent to RFLP (Restriction Fragment Length Polymorphism), this method is an alternative to IS6110-RFLP for epidemiological studies (Mazars et al , 2001; Supply et al , 2000).…”

Section: Resultsmentioning

confidence: 99%

“…This technique has limitations, and it could be combined with an additional genotyping method for greater accuracy (Cowan et al , 2005; Van Deutekom et al , 2005; Kang et al , 2009). Different combinations of MIRU and other loci have been evaluated.…”

Section: Resultsmentioning

confidence: 99%

Mycobacterium tuberculosis belonging to family LAM and sublineage RD Rio: common strains in Southern Brazil for over 10 years

Soares

Macedo

Groll

et al. 2013

Braz. J. Microbiol.

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A sublineage of Mycobacterium tuberculosis called RDRio was described in 2007. Although only recently described, this strain may have been present previously in the population, and its identification in clinical isolates will elucidate bacterial transmission dynamics and host-pathogen interactions. This study evaluated the clonal diversity of the RDRio sublineage in clinical isolates from Rio Grande-RS obtained between 1998 and 2001. Among the 45 samples analyzed by the MIRU-VNTR method, there were six clusters with two samples each and 33 orphan strains with unique pattern. The strains were distributed across several different lineages including LAM (34.04%), × (14.89%), Haarlem (12.77%), UgandaI (10.64%), S (4.26%), NEW-1 (2.13%) and Cameroon (2.13%); 14.89% of the strains matched to multiple lineages. RDRio strains were present in 28.9% of the samples and 81.25% of the identified strains belonged to the LAM family. The high clonal diversity observed in this study is a constant feature in this region. The RDRio sublineage has been in Rio Grande-RS since 1998. The continued monitoring of RDRio in clinical isolates will enhance the understanding of its epidemiological significance.

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“…Interestingly, the Beijing family strains tested in this study harbored relatively small numbers of IS 6110 bands (8 to 21), indicating that the characteristics of the Beijing family isolated in Korea are different from those isolated in other countries. Mycobacterial interspersed repetitive units (MIRU)-variable number of tandem repeats (VNTR), another molecular epidemiologic tool for M. tuberculosis , was introduced to genotype M. tuberculosis isolates from Korea ( 4 , 27 ). The MIRU-VNTR is highly discriminatory and easy to use, and can differentiate strains belonged to the Beijing family ( 4 ).…”

Section: Discussionmentioning

confidence: 99%

IS6110-Restriction Fragment Length Polymorphism and Spoligotyping Analysis ofMycobacterium tuberculosisClinical Isolates for Investigating Epidemiologic Distribution in Korea

et al. 2010

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The Beijing family of Mycobacterium tuberculosis has been emerging in the world. However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates of M. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M. tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview of the distribution of genotypes of M. tuberculosis in Korea. These findings indicate that we have to pay more attention to control of M. tuberculosis strains associated with the Beijing family.

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Evaluation of the Selected 12-locus MIRU for Genotyping Beijing Family Mycobacterium tuberculosis in Korea

Cited by 4 publications

References 22 publications

High Prevalence of Beijing and EAI4-VNM Genotypes among M. tuberculosis Isolates in Northern Vietnam: Sampling Effect, Rural and Urban Disparities

High Prevalence of Beijing and EAI4-VNM Genotypes among M. tuberculosis Isolates in Northern Vietnam: Sampling Effect, Rural and Urban Disparities

Mycobacterium tuberculosis belonging to family LAM and sublineage RD Rio: common strains in Southern Brazil for over 10 years

IS6110-Restriction Fragment Length Polymorphism and Spoligotyping Analysis ofMycobacterium tuberculosisClinical Isolates for Investigating Epidemiologic Distribution in Korea

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