Evaluation of the role of respiratory syncytial virus surface glycoproteins F and G on viral stability and replication: implications for future vaccine design

DeFord, Darby; Nosek, J; Castiglia, Katelyn R.; Hasik, Emily F.; Franke, Megan E.; Nick, Benjamin; Abdelnour, Alyssa M.; Haas, Caitlin E.; Junod, Nathan A.; Latsko, Karina N.; Moore, Martin L.; Berthrong, Sean T.; Rostad, Christina A.; Stobart, Christopher C.

doi:10.1099/jgv.0.001287

Cited by 7 publications

(12 citation statements)

References 43 publications

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“…Viral decay is known to be affected by not only temperature, but other environmental factors such as pH (DeFord et al. 2019 ; Randazzo et al. 2017 ), relative humidity (Yeargin et al.…”

Section: Discussionmentioning

confidence: 99%

“…( 2010 ) Influenza 1 DeFord et al. ( 2019 ) RSV 4 Hambling ( 1964 ) RSV 2, 3, 4, 5 Rechsteiner ( 1968 ) RSV 1, 2 Casanova et al. ( 2010 ) TGEV & MHV 1, 2, 3 Chan et al.…”

Section: Methodsunclassified

“…Finally, our ability to effectively compare the inactivation energies for different viruses is limited by the inconsistency in experimental conditions. Viral decay is known to be affected by not only temperature, but other environmental factors such as pH (DeFord et al 2019;Randazzo et al 2017), relative humidity (Yeargin et al 2016), and pressure (Daher et al 2017).…”

Section: ) Gostingmentioning

confidence: 99%

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Energy Requirements for Loss of Viral Infectivity

Rowell

Dobrovolny

2020

Food Environ Virol

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Outside the host, viruses will eventually lose their ability to infect cells due to conformational changes that occur to proteins on the viral capsid. In order to undergo a conformational change, these proteins require energy to activate the chemical reaction that leads to the conformational change. In this study, data from the literature is used to calculate the energy required for viral inactivation for a variety of different viruses by means of the Arrhenius equation. We find that some viruses (rhinovirus, poliovirus, human immunodeficiency virus, Alkhumra hemorrhagic fever virus, and hepatitis A virus) have high inactivation energies, indicative of breaking of a chemical double bond. We also find that several viruses (respiratory syncytial virus, poliovirus, and norovirus) have nonlinear Arrhenius plots, suggesting that there is more than a single pathway for inactivation of these viruses.

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“…Viral decay is known to be affected by not only temperature, but other environmental factors such as pH (DeFord et al. 2019 ; Randazzo et al. 2017 ), relative humidity (Yeargin et al.…”

Section: Discussionmentioning

confidence: 99%

“…( 2010 ) Influenza 1 DeFord et al. ( 2019 ) RSV 4 Hambling ( 1964 ) RSV 2, 3, 4, 5 Rechsteiner ( 1968 ) RSV 1, 2 Casanova et al. ( 2010 ) TGEV & MHV 1, 2, 3 Chan et al.…”

Section: Methodsunclassified

Section: ) Gostingmentioning

confidence: 99%

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Energy Requirements for Loss of Viral Infectivity

Rowell

Dobrovolny

2020

Food Environ Virol

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“…Unsurprisingly, fluctuations in air pressure, humidity, solar radiation, temperature, and wind speed have been associated with exacerbations of a number of respiratory diseases, including chronic obstructive pulmonary disease (COPD) and asthma [ 19 , 20 , 21 , 22 ]. In addition, meteorological conditions such as radiation (e.g., solar UV light) may impact viral stability and possibly the patient’s viral load [ 23 , 24 ], thereby influencing the risk of severe RSV bronchiolitis and PICU admission [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

The Association between Weather Conditions and Admissions to the Paediatric Intensive Care Unit for Respiratory Syncytial Virus Bronchiolitis

et al. 2021

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Respiratory syncytial virus (RSV) bronchiolitis is a leading cause of global child morbidity and mortality. Every year, seasonal RSV outbreaks put high pressure on paediatric intensive care units (PICUs) worldwide, including in the Netherlands, and this burden appears to be increasing. Weather conditions have a strong influence on RSV activity, and climate change has been proposed as a potential important determinant of future RSV-related health care utilisation. In this national study spanning a total of 13 years with 2161 PICU admissions for RSV bronchiolitis, we aimed (1) to identify meteorological variables that were associated with the number of PICU admissions for RSV bronchiolitis in the Netherlands and (2) to determine if longitudinal changes in these variables occurred over time as a possible explanation for the observed increase in PICU burden. Poisson regression modelling was used to identify weather variables (aggregated in months and weeks) that predicted PICU admissions, and linear regression analysis was used to assess changes in the weather over time. Maximum temperature and global radiation best predicted PICU admissions, with global radiation showing the most stable strength of effect in both month and week data. However, we did not observe a significant change in these weather variables over the 13-year time period. Based on our study, we could not identify changing weather conditions as a potential contributing factor to the increased RSV-related PICU burden in the Netherlands.

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“…Major differences have been previously observed in the properties of RSV strains during propagation in cell culture, including syncytium size and phenotype, virus titers, and thermal stability (26)(27)(28). In general, RSV-A strains have been shown to attain higher titers in cell culture with increased plaque size in comparison with RSV-B strains (29).…”

mentioning

confidence: 99%

Reverse genetics systems for contemporary isolates of respiratory syncytial virus enable rapid evaluation of antibody escape mutants

Schadenhofer

Habierski

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Human respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory infection in children under 5 y of age. In the absence of a safe and effective vaccine and with limited options for therapeutic interventions, uncontrolled epidemics of RSV occur annually worldwide. Existing RSV reverse genetics systems have been predominantly based on older laboratory-adapted strains such as A2 or Long. These strains are not representative of currently circulating genotypes and have a convoluted passage history, complicating their use in studies on molecular determinants of viral pathogenesis and intervention strategies. In this study, we have generated reverse genetics systems for clinical isolates of RSV-A (ON1, 0594 strain) and RSV-B (BA9, 9671 strain) in which the full-length complementary DNA (cDNA) copy of the viral antigenome is cloned into a bacterial artificial chromosome (BAC). Additional recombinant (r) RSVs were rescued expressing enhanced green fluorescent protein (EGFP), mScarlet, or NanoLuc luciferase from an additional transcription unit inserted between the P and M genes. Mutations in antigenic site II of the F protein conferring escape from palivizumab neutralization (K272E, K272Q, S275L) were investigated using quantitative cell-fusion assays and rRSVs via the use of BAC recombineering protocols. These mutations enabled RSV-A and -B to escape palivizumab neutralization but had differential impacts on cell-to-cell fusion, as the S275L mutation resulted in an almost-complete ablation of syncytium formation. These reverse genetics systems will facilitate future cross-validation efficacy studies of novel RSV therapeutic intervention strategies and investigations into viral and host factors necessary for virus entry and cell-to-cell spread.

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Evaluation of the role of respiratory syncytial virus surface glycoproteins F and G on viral stability and replication: implications for future vaccine design

Cited by 7 publications

References 43 publications

Energy Requirements for Loss of Viral Infectivity

Energy Requirements for Loss of Viral Infectivity

The Association between Weather Conditions and Admissions to the Paediatric Intensive Care Unit for Respiratory Syncytial Virus Bronchiolitis

Reverse genetics systems for contemporary isolates of respiratory syncytial virus enable rapid evaluation of antibody escape mutants

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