Evaluation of the Response Characteristics of On-Chip Gel Actuators for Various Single Cell Manipulations

Wada, Hiroki; Koike, Yuha; Yokoyama, Yoshihiko; Hayakawa, Takeshi

doi:10.1109/lra.2020.3006792

“…However, contact methods incur the risk of cell damage when the microstructures contact the target cells. To overcome these problems, on-chip gel actuators that have a similar softness to cells are attracting considerable attention [13][14][15][16][17][18][19]. Although they use a contact method, on-chip gel actuators cause less physical damage to cells because they are soft.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, by controlling the temperature of the PNIPAAm, we can use this volume change as an actuator. Previously, we proposed a method for driving an on-chip gel actuator made of PNIPAAm using light irradiation [18,19]. In this method, gel actuators are fabricated on a glass substrate with a light-absorbing pattern.…”

Section: Introductionmentioning

confidence: 99%

“…1a. In our previous work [18,19], we succeeded in integrating a large number of gel actuators into a microfluidic chip and driving multiple actuators by irradiating the integrated gel actuators with patterned light. Additionally, we used the integrated gel actuators to demonstrate various cell manipulations, including cell transporting, trapping and sorting.…”

Section: Introductionmentioning

confidence: 99%

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Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators

Koike

¹

,

Kodera

²

,

Yokoyama

³

et al. 2022

Robomech J

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1

0

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A light-driven gel actuator is a potential candidate for a single-cell manipulation tool because it allows cells to be manipulated while ensuring less damage. Moreover, a large number of actuators can be integrated into a microfluidic chip because no wiring is required. Previously, we proposed a method for cell manipulation using light-driven gel actuators. However, the system used in the previous work did not allow the targeted cells to be manipulated in real time because the system used in the previous work could only irradiate preprogrammed patterned light. Moreover, when a large number of gel actuators are integrated into a chip, the Gaussian distribution of the laser light source results in the response characteristics of the gel actuators varying with the location of the actuator. In this work, we constructed a system that homogenized the intensity of the patterned light used for irradiation, allowing multiple gel actuators to be driven in parallel in real time. The intensity-homogenized patterned light improved the variations in the response characteristics of the gel actuators, and as a result, we succeeded in actuating gel actuators with various light patterns in real time.

show abstract

“…1 (a). In our previous work [18,19], we succeeded in integrating a large number of gel actuators into a microfluidic chip and driving multiple actuators by irradiating the integrated gel actuators with patterned light. Additionally, we used the integrated gel actuators to demonstrate various cell manipulations, including cell transporting, trapping and sorting.…”

Section: Introductionmentioning

confidence: 99%

Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators

Koike

¹

,

Kodera

²

,

Yokoyama

³

et al. 2021

Preprint

Self Cite

0

View full text Add to dashboard Cite

A light-driven gel actuator is a potential candidate for a single-cell manipulation tool because it allows cells to be manipulated while ensuring less damage. Moreover, a large number of actuators can be integrated into a microfluidic chip because no wiring is required. Previously, we proposed a method for cell manipulation using light-driven gel actuators. However, the system used in the previous work did not allow the targeted cells to be manipulated in real time because the system used in the previous work could only irradiate preprogrammed patterned light. Moreover, when a large number of gel actuators are integrated into a chip, the Gaussian distribution of the laser light source results in the response characteristics of the gel actuators varying with the location of the actuator. In this work, we constructed a system that homogenized the intensity of the patterned light used for irradiation, allowing multiple gel actuators to be driven in parallel in real time. The intensity-homogenized patterned light improved the variations in the response characteristics of the gel actuators, and as a result, we succeeded in actuating gel actuators with various light patterns in real time.

show abstract

Studying and simulation of ellipsoidal contact models for application in AFM nano manipulation

Korayem

¹

,

Panahi

²

,

Khaksar

³

2021

Micron

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Evaluation of the Response Characteristics of On-Chip Gel Actuators for Various Single Cell Manipulations

Cited by 6 publications

References 33 publications

Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators

Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators

Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators

Studying and simulation of ellipsoidal contact models for application in AFM nano manipulation

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