Evaluation of the resistance of transgenic potato plants expressing various levels of Cry3A against the Colorado potato beetle (Leptinotarsa decemlineataSay) in the laboratory and field

Abstract

BACKGROUND: The Colorado potato beetle (CPB), Leptinotarsa decemlineata Say, is a destructive pest. The CPB is a quarantine pest in China, but has now invaded the Xinjiang Uygur Autonomous Region and is continuing to spread eastwards. To control the damage and overspreading, transgenic potato plants expressing Cry3A toxin were developed, and their resistance to CPB was evaluated by bioassays in the laboratory and field in 2009, 2010 and 2011.RESULTS: The insect resistance of the high‐dose (HD) transgenic lines was significantly greater than the middle‐dose (MD) and low‐dose (LD) transgenic lines regarding leaf consumption, biomass accumulation and mortality. The HD and MD transgenic lines showed 100% mortality when inoculated with first‐ and second‐instar larvae; however, the LD transgenic lines showed about 50% mortality. The HD transgenic lines exhibited a significantly higher yield than the MD and LD transgenic lines owing to their high CPB resistance.CONCLUSION: Commercially available transgenic potato plants with above 0.1% Cry3A of total soluble protein and NT control refugia could control damage, delay adaptation and halt dispersion eastwards. The two HD transgenic lines developed in this study, PAH1 and PAH2, are ideal for use as cultivars or germplasm to breed new cultivars. Copyright © 2012 Society of Chemical Industry

Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle

“…The insect resistance bioassays were performed according to methods described by Cooper et al [26], with minor modifications [10,41]. The potato plants in vitro, grown for about 25 days, were detached from the top of the transgenic and control plants with about 75 mm high.…”

Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice

“…Bacillus thuringiensis 균주로부터 분리한 cryIII 유전자는 딱정벌레목 해충에 살충성을 가지고 있어 효율적으로 딱 정벌레목 해충을 방제 할 수 있다고 알려져 있으며 (Hoefte and Whiteley 1989;Krieg et al 1989), cryIII 유전자를 이용 한 형질전환으로 토마토 (Rhim et al 1995), 감귤 (Rhim et al 2004), 감자 (Jouzani et al 2008), 고구마 (Gonza´lez et al 2008), 옥수수 (Hibbard et al 2010) 등의 연구가 보고되었 고, 또한 cry3A 유전자를 도입한 감자와 알팔파의 기내 및 포장실험 실험에서 딱정벌레목에 대한 살충성이 확인 되었다 (Zhou et al 2012;Tohidfar et al 2013). 최근 국립농업과학원에서는 cry3A 유전자 형질전환 벼 를 육성하였으며, GMO 포장 내 생물검정 연구를 통해 벼 물바구미에 대한 내충성을 확인하였다 (Lee et al 2013 의 벼물바구미 내충성 벼를 실험재료로 사용하였고, 대 조체로는 형질전환 모본인 낙동벼(Oryza sativa cv.…”

Development of selectable marker-free transgenic potato plants expressingcry3Aagainst the Colorado potato beetle (Leptinotarsa decemlineataSay)

“…In the 1990s, CPB began to invade China from Kazakhstan. Since then, it has spread eastwards and is currently east of the Xinjiang Uygur Autonomous Region . Damage from CPB can reduce annual potato yield by 30–50%, and no yield may be reported in severely damaged fields.…”

“…In the last 20 years, transgenic plants have been widely planted to control the damage caused by a range of pests, e.g. transgenic cotton expressing cry1Ac conferred resistance to Helicoverpa armigera (Hübner), transgenic maize with Cry1Ab killed Ostrinia nubilalis (Hübner) and transgenic potato with Cry3A conferred resistance to CPB . Although transgenic potatoes with the Cry3A toxin showed high resistance to CPB and were commercially available in the United States from 1996, these potatoes were taken off the market in 2001 owing to consumer concerns over the safety of transgenic plants, particularly the safety of SMGs .…”

“…In our laboratory, we previously developed transgenic cry3A potato plants with high resistance to CPB; however, the transgenic potato contains the SMG npt II , which is now prohibited from entering commercial markets in China. Thus, in this study, we developed a selectable marker‐free transgenic potato expressing the Cry3A protein to improve safety with the ultimate goal of commercial use.…”