Evaluation of the reproducibility of a serological test for antibodies to Chlamydia trachomatis pgp3: A potential surveillance tool for trachoma programs

Kaur, Hemjot; Dize, Laura; Muñoz, Beatriz; Gaydos, Charlotte A.; West, Sheila K.

doi:10.1016/j.mimet.2018.02.017

Cited by 16 publications

(11 citation statements)

References 4 publications

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“…Seroepidemiology based on highly specific and sensitive assays can determine past exposure and measure the cumulative risk of C. trachomatis infection over time ( 4 – 7 ) and thus can help to minimize and control the burden of C. trachomatis infections in populations ( 71 ). The high performance of multipeptide ELISAs provides a powerful argument for their use in trachoma control ( 24 , 26 , 27 ) and in screening for sexually transmitted infections ( 21 , 25 , 28 ). Such multipeptide ELISAs will increase the effectiveness of trachoma surveillance programs, and they will enable earlier identification and treatment of young women with chlamydial infection in sexually transmitted disease (STD) screening, reducing a woman’s likelihood of experiencing highly consequential reproductive health complications.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Molecular Serology of HumanChlamydia trachomatisInfections by Peptide Enzyme-Linked Immunosorbent Assays

Rahman

Darville

Russell

et al. 2018

mSphere

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For detection of anti-Chlamydia trachomatis antibodies by serological assays, use of classical whole-organism chlamydial antigens results in high cross-reactivity. These antigens bind mainly antibodies against the major outer membrane protein (OmpA) and bind antibodies against other immunodominant non-OmpA proteins to a lesser extent, resulting in poor assay sensitivity. The specificity of C. trachomatis serology is also compromised by the high prevalence of cross-reactive anti-C. pneumoniae antibodies in human populations. We previously identified 48 highly specific C. trachomatis B cell epitope peptide antigens of 21 immunodominant proteins. This study validated peptide antigen-based novel ELISAs that provide highly specific and sensitive detection of anti-C. trachomatis antibodies. Compared to four commercial ELISAs that achieved only poor sensitivities (51.5% to 64.8%), the combined signals of 5 to 11 peptides provided high sensitivity (86.5% to 91.8%) at the same 98% specificity. Thus, by using multiple peptide antigens of immunodominant proteins, we created simple ELISAs with specificity and sensitivity superior to standard C. trachomatis serodiagnosis.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Molecular Serology of HumanChlamydia trachomatisInfections by Peptide Enzyme-Linked Immunosorbent Assays

Rahman

Darville

Russell

et al. 2018

mSphere

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“…Such tools can expedite ongoing efforts to minimize and control the burden of C. trachomatis infections in populations ( 46 ). The high performance of this multipeptide ELISA and the convenience of this method provide a strong argument for their use in trachoma control ( 22 , 23 , 47 – 49 ) and in screening for sexually transmitted infections ( 10 , 50 , 51 ). In conclusion, this highly specific and sensitive peptide ELISA will provide simple and yet improved C. trachomatis serology for epidemiological studies and will enhance accurate diagnosis of C. trachomatis infections, thereby mitigating sequelae from C. trachomatis infections ( 52 – 59 ).…”

Section: Discussionmentioning

confidence: 99%

Mixed Chlamydia trachomatis Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti-C. trachomatis Antibodies

Rahman

Darville

Wiesenfeld

et al. 2018

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For detection of anti-C. trachomatis antibodies by serological assays, use of classical chlamydial antigens results in high cross-reactivity and poor sensitivity. Previously, we discovered 48 strongly reactive peptide antigens of C. trachomatis-specific B-cell epitopes from 21 immunodominant proteins, and individual testing and combined scoring of 5 to 11 peptide antigens provided highly sensitive and specific detection of anti-C. trachomatis antibodies in chemiluminescent ELISAs. To simplify this method, this study established a single-well labor-saving colorimetric ELISA using a mixture of 12 strongly reactive C. trachomatis peptide antigens (Ctr Mix1) for detection of anti-C. trachomatis antibodies. This Ctr Mix1 ELISA (94% sensitivity and 98% specificity) outperformed 4 commercial ELISAs (49% to 79% sensitivity and 98% specificity). This ELISA can be easily implemented and commercialized, with convenient setup for use in nonspecialized laboratories. Thus, this mixed peptide assay with superior specificity and sensitivity will improve serodiagnosis of C. trachomatis infections.

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“…The test using the multiplex bead array platform is reliable, even when the same samples are tested months apart. 28 However, there is evidence of seroreversion. In a longitudinal cohort of 2000 Tanzanian children in 50 communities followed up over a year, most children either retained their baseline antibody status or seroconverted.…”

Section: When: Determining When the Elimination Goal Has Been Achievedmentioning

confidence: 99%

Toward the Elimination of Disease: the 2019 Weisenfeld Award Lecture

West

2019

Invest. Ophthalmol. Vis. Sci.

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Evaluation of the reproducibility of a serological test for antibodies to Chlamydia trachomatis pgp3: A potential surveillance tool for trachoma programs

Cited by 16 publications

References 4 publications

Comprehensive Molecular Serology of HumanChlamydia trachomatisInfections by Peptide Enzyme-Linked Immunosorbent Assays

Comprehensive Molecular Serology of HumanChlamydia trachomatisInfections by Peptide Enzyme-Linked Immunosorbent Assays

Mixed Chlamydia trachomatis Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti-C. trachomatis Antibodies

Toward the Elimination of Disease: the 2019 Weisenfeld Award Lecture

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