Evaluation of the Qualitative and Automated Quantitative Microhemagglutination Assay for Antibodies to Treponema pallidum

Coffey, E. M.; Bradford, L. L.; Naritomi, L. S.; Wood, R. M.

doi:10.1128/aem.24.1.26-30.1972

Cited by 12 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chronic BFP test results have been attributed to many different conditions (e.g., diabetes, rheumatoid arthritis, lupus, pregnancy, apheresis donation) 1–9 . Acute or transient BFP serologic tests for syphilis (STS) results have been associated with strong immunologic responses (i.e., acute bacterial or viral infections, some vaccinations) 1–9 1–4 and treponemal tests 4–9 …”

mentioning

confidence: 99%

See 1 more Smart Citation

Absence of risk factors for false‐positive test results inblood donors with a reactive test result inan automated treponemal test (PK‐TP) for syphilis

2001

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

“…Acute or transient BFP serologic tests for syphilis (STS) results have been associated with strong immunologic responses (i.e., acute bacterial or viral infections, some vaccinations) 1–9 1–4 and treponemal tests 4–9 …”

mentioning

confidence: 99%

Absence of risk factors for false‐positive test results inblood donors with a reactive test result inan automated treponemal test (PK‐TP) for syphilis

2001

View full text Add to dashboard Cite

show abstract

“…Although nontreponemal tests are less specific, they are more simple to perform and are of greatest value when used as screening procedures and for following therapy. Many studies have shown that there is good agreement between treponemal and nontreponemal tests (2,6). Because nontreponemal tests are simple, rapid, reproducible, and inexpensive, they can be performed in a variety of clinical laboratories and have been widely used.…”

mentioning

confidence: 99%

Dot-immunogold filtration assay as a screening test for syphilis

et al. 1996

View full text Add to dashboard Cite

A dot-immunogold filtration assay (DIGFA) for the rapid detection of reaginic antibody in the serum of syphilitic patients was developed. The assay was simple, rapid, and reproducible. The test completion time was 2 min, and the assay required no equipment. The positive dot was very obvious, and the results could easily be determined with the naked eye. A total of 350 serum samples were examined by DIGFA, the rapid plasma reagin test, and the fluorescent treponemal antibody-absorption test. The levels of agreement between DIGFA and the rapid reagin test and between DIGFA and the fluorescent treponemal antibody-absorption test were 100 and 98%, respectively. The results of clinical application indicated that DIGFA could be used as a routine screening test for syphilis.

show abstract

Sensitivity and Specificity of Treponemal-specific Tests for the Diagnosis of Syphilis

Park

Tran

Pereira

et al. 2020

Clinical Infectious Diseases

View full text Add to dashboard Cite

We conducted a systematic review of relevant syphilis diagnostic literature to address the question, “What is the sensitivity and specificity of the treponemal tests currently approved by the Food and Drug Administration (FDA) for the diagnosis of syphilis (by stage)?” There were 16 treponemal assays evaluated: 13 immunoassays and 3 manual assays (fluorescent treponemal antibody absorbed test [FTA-ABS], microhemagglutination assay for Treponema pallidum antibodies [MHA-TP], Treponema pallidum particle agglutination assay [TP-PA]). MHA-TP and FTA-ABS were less sensitive in primary and secondary syphilis than TP-PA; TP-PA is the most specific manual treponemal assay. There is insufficient evidence to recommend one particular treponemal immunoassay (eg, enzyme immunoassays, chemiluminescence immunoassays, microbead immunoassays) over another based on published performance data. For diagnosis of neurosyphilis, cerebrospinal fluid (CSF) TP-PA has similar performance to CSF FTA-ABS in studies with patients with definitive or presumptive neurosyphilis. However, CSF treponemal testing has limitations in its sensitivity and specificity and should be interpreted within the context of the clinical scenario, additional CSF test results and syphilis prevalence.

show abstract

Evaluation of the Qualitative and Automated Quantitative Microhemagglutination Assay for Antibodies to Treponema pallidum

Cited by 12 publications

References 7 publications

Absence of risk factors for false‐positive test results inblood donors with a reactive test result inan automated treponemal test (PK‐TP) for syphilis

Absence of risk factors for false‐positive test results inblood donors with a reactive test result inan automated treponemal test (PK‐TP) for syphilis

Dot-immunogold filtration assay as a screening test for syphilis

Sensitivity and Specificity of Treponemal-specific Tests for the Diagnosis of Syphilis

Contact Info

Product

Resources

About