Evaluation of the protective immune response induced in mice by immunization with Schistosoma mansoni schistosomula tegument (Smteg) in association with CpG-ODN

Melo, Tatiane Teixeira de; Araújo, Juliano Michel de; Romano, Isabela Cristina Sena; Alves, Clarice Carvalho; Araújo, Neusa; Fonseca, Cristina Toscano

doi:10.1016/j.micinf.2012.10.007

Cited by 18 publications

(10 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, studies performed by our group have demonstrated that mice immunization with Smteg in different vaccine formulations induced a significant production of specific antibodies . Whether these antibodies were directly involved in parasite elimination or only represent a hallmark of host immune response activation still undetermined.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, our group demonstrated that Schistosoma mansoni schistosomula (Smteg) tegument plays an important role in triggering the immune response against the parasite. This antigen is able to activate upregulation of CD40 and CD86 expressions in dendritic cells and induce a partial protection in mice when formulated with Freund's adjuvant (43–48%) or alum + CpG‐ODN . The protective response was associated with a predominant Th1 type of immune response, with increased production of specific antibodies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization

Melo

Sena

Araújo

et al. 2014

Parasite Immunology

View full text Add to dashboard Cite

The Schistosoma mansoni schistosomula tegument (Smteg) plays an important role in triggering the host immune response and mice immunization with Smteg formulated with Freunds adjuvant or alum + CpG induce partial protection against S. mansoni infection associated with an increased antibody production. In this study, we investigated the role of these antibodies in parasite killing both in vitro and in vivo. We demonstrated that these antibodies were able to bind to the surface of S. mansoni recently transformed schistosomula and that these antibodies significantly increase the percentage of schistosomula killed in vitro by complement activation. Passive transference of immune sera decreased the parasite burden and the number of eggs trapped in the organs of mice that received sera containing anti-Smteg antibodies. These results demonstrate that antibodies specific to surface tegumental antigens are involved in parasite elimination in mice immunized with Smteg.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization

Melo

Sena

Araújo

et al. 2014

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Teg antigen induced a 35.2–39.9% reduction in adult worms when compared to the PBS + adjuvant and this level of protection was similar to that obtained by Roberts et al (1988), who reported 39.8% protection using purified adult tegumental membranes plus Freund's adjuvant, and with Smithers et al (1989), who showed that adult worm surface membranes in combination with saponin resulted in 18–37% (average 26%) fewer adult worms than in control mice. Also, immunization with S. mansoni schistosomula tegument yielded a 43–48% reduction in worm burden compared to the control group when used with Freund's adjuvant (Teixeira de Melo et al , 2010; Araujo et al ., 2012) and 43.1% when combined with alum/synthetic unmethylated CpG oligodeoxynucleotides (Teixeira de Melo et al , 2013).…”

Section: Discussionmentioning

confidence: 99%

Immunization with adultSchistosoma mansonitegument, treated with sub-curative praziquantel, partially protects mice against the infection

et al. 2019

View full text Add to dashboard Cite

The tegument of schistosomes is a source of many potential anti-Schistosoma vaccine molecules. This work aimed to assess the protective effects of the adult Schistosoma mansoni tegument treated (TT) with sub-curative praziquantel (PZQ), whether in vivo (in vivo TT) or in vitro (in vitro TT), in murine schistosomiasis. In vitro TT and in vivo TT showed great similarity, and they differed from untreated tegument antigen (Teg) in terms of quantity and quality of protein bands on SDS–PAGE. Two immunization trials were performed, each with 50 mice, divided randomly into five groups of 10 mice each: (1) uninfected control mice (UC), (2) infected mice given phosphate buffer saline + adjuvant (PBS + adjuvant), (3) infected, Teg vaccinated, (4) infected, in vivo TT vaccinated, and (5) infected, in vitro TT vaccinated. All the immunizations with antigens induced mixed Th1/Th2 immune responses, as indicated by significantly high (P < 0.001) specific IgG2a and IgG1 levels, with Th1 predominating, as shown by a diminished IgG1/IgG2a ratio, as well as a high serum concentration of IFN-γ, an absence of IL-4 and increased IL-10. In vitro TT gave the most pronounced response. With respect to reduction of total worm burden, relative to PBS + adjuvant mice, in vitro TT achieved the highest significant (P < 0.001) results, followed by in vivo TT and Teg (51.8–57.04%, 44.6–50.2% and 35.2–39.3%, respectively). In scanning electron microscopy studies, all the tested antigens caused tegumental changes in adult worms, with the worst occurring with in vitro TT, such as retracted ventral sucker, an effect on the gynaecophoric canal, and changes to tubercles. In conclusion, in vitro TT, which is cheap to prepare, could be a potential vaccine against S. mansoni.

show abstract

“…It is important to stress that, in this study, we measured IFNγ and IL-4 levels in splenic CD4 + T cells isolated from vaccinated mice at 6 weeks post-challenge when S. japonicum worms are fully mature and have already laid considerable numbers of eggs. In many previous studies, analysis of the immune response was performed immediately after the completion of the vaccination regimen and prior to cercarial challenge, and the results obtained indicated the protective immune response induced by vaccination was associated with a predominant Th1 response [ 20 , 21 ]. At 6-week post-challenge, we found significantly enhanced IFNγ levels with increased ratio of Th1/Th2 ( Figure 4 ) were produced in splenic CD4 + T cells of both rSjLD1-ISA ( Figure 2 b) and rSjLD1-QuilA ( Figure 3 a) vaccinated mice when stimulated with rSjLD1, reflecting the increased Th1 response after being inhibited by a strong Th2 response elicited by newly-laid eggs.…”

Section: Discussionmentioning

confidence: 99%

Protective Immune Responses Generated in a Murine Model Following Immunization with Recombinant Schistosoma japonicum Insulin Receptor

You

Harvie

et al. 2018

IJMS

View full text Add to dashboard Cite

There is a pressing need to develop vaccines for schistosomiasis given the current heavy dependency on praziquantel as the only available drug for treatment. We previously showed the ligand domain of the Schistosoma japonicum insulin receptor 1 and 2 (rSjLD1 and 2) fusion proteins conferred solid protection in mice against challenge infection with S. japonicum. To improve vaccine efficacy, we compared the immunogenicity and protective efficacy of rSjLD1 on its own and in combination with S. japonicum triose-phosphate isomerase (SjTPI), formulated with either of two adjuvants (QuilA and montanide ISA 720VG) in murine vaccine trials against S. japonicum challenge. The level of protection was higher in mice vaccinated only with rSjLD1 formulated with either adjuvant; rSjTPI or the rSjTPI-rSjLD1 combination resulted in a lower level of protection. Mirroring our previous results, there were significant reductions in the number of female worms (30–44%), faecal eggs (61–68%), liver eggs (44–56%), intestinal eggs (46–48%) and mature intestinal eggs (58–63%) in the rSjLD1-vaccinated mice compared with the adjuvant only groups. At 6-weeks post-cercarial challenge, a significantly increased production of interferon gamma (IFNγ) in rSjLD1-stimulated splenic CD4+ T cells was observed in the rSjLD1-vaccinated mice suggesting a Th1-type response is associated with the generated level of protective efficacy.

show abstract

Evaluation of the protective immune response induced in mice by immunization with Schistosoma mansoni schistosomula tegument (Smteg) in association with CpG-ODN

Cited by 18 publications

References 48 publications

Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization

Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization

Immunization with adultSchistosoma mansonitegument, treated with sub-curative praziquantel, partially protects mice against the infection

Protective Immune Responses Generated in a Murine Model Following Immunization with Recombinant Schistosoma japonicum Insulin Receptor

Contact Info

Product

Resources

About