Evaluation of the Protective Effects of Chinese Herbs against Biomolecule Damage Induced by Peroxynitrite

Luo, Yunjing; Pan, Jinglin; Pan, Yanshu; Han, Zhiguo; Zhong, Rugang

doi:10.1271/bbb.90914

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…The mean inhibition rates of batches S1, S2 and S14 were 66.78%, 84.09% and 72.87% respectively. The inhibition rates of all the three batches were >60%, compared with 32 traditional Chinese herbs were reported previously, 29 which indicated the ability for lotus leaf to inhibit protein tyrosine nitration.…”

Section: Resultssupporting

confidence: 65%

A novel analytical method based on HPLC-PDA coupled post-column derivatization to evaluate the ability to inhibit tyrosine nitration in lotus leaf extracts

Yin

Chen

et al. 2018

RSC Adv.

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Section: Resultssupporting

confidence: 65%

A novel analytical method based on HPLC-PDA coupled post-column derivatization to evaluate the ability to inhibit tyrosine nitration in lotus leaf extracts

Yin

Chen

et al. 2018

RSC Adv.

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“…Peroxynitrite, formed in a rapid reaction between superoxide anion (O À 2 ) and nitric oxide (NO), is able to modify other molecules (proteins, lipids, and nucleic acids) by diverse mechanisms, including both direct reactions and damage induced by secondary radicals (Pacher et al, 2007). Because of the lack of specific enzymes responsible for deactivation of ONOO À in human tissues, scavenging of this molecule and reduction of its harmful effects depends mainly on endogenous and exogenous non-enzymatic antioxidants (Luo et al, 2010). It has been estimated that under in vitro conditions, the bolus addition of 0.25 mM ONOO À is equivalent to a steady-state level of 1 mM maintained for 7 min (Beckman et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Biological activity of clovers – Free radical scavenging ability and antioxidant action of sixTrifoliumspecies

Kołodziejczyk-Czepas

Nowak

Kowalska

et al. 2014

Pharmaceutical Biology

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Context: Clovers were chosen on the basis of traditional medicine recommendations, agricultural value, or available information on their promising chemical profiles.Objective: This study evaluates and compares free radical scavenging and antioxidant properties of six clover species: Trifolium alexandrinum L. (Leguminosae), Trifolium fragiferum L., Trifolium hybridum L., Trifolium incarnatum L., Trifolium resupinatum var. majus Boiss., and Trifolium resupinatum var. resupinatum L. Materials and methods: Free radical scavenging activity of the extracts (1.5-50 mg/ml) was estimated by reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH ) and 2,2 0 -azino-bis(3-ethylbenzthiazoline-6-sulphonic) acid (ABTS ) radicals. The Trifolium extract effects on total antioxidant capacity of blood plasma were determined by the reduction of ABTS + and DPPH radicals, as well as with the use of the ferric reducing ability of plasma (FRAP) assay. Results: The UPLC analysis of chemical profiles of the examined extracts showed the presence of three or four groups of phenolic substances, including phenolic acids, clovamides, isoflavones, and other flavonoids. The measurements of free radical scavenging and ferric reducing ability of the examined clover extracts revealed the strongest effect for T. alexandrinum. Furthermore, antioxidant activity assays in human plasma have shown protective effects of all extracts against peroxynitrite-induced reduction of total antioxidant capacity. Conclusions: Trifolium plants may be a rich source of bioactive substances with antioxidant properties. The examined extracts displayed free radical scavenging action and partly protected blood plasma against peroxynitrite-induced oxidative stress; however, the beneficial effects of T. alexandrinum and T. incarnatum seem to be slightly higher.

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“…Modern pharmacological research has revealed some important compounds such as paeoniflorin (PF) and gallic acid (GA) in radix paeoniae alba; vanillic acid (VA) and ferulic acid (FA) in ligusticum chuanxiong and angelica sinensis; liquiritin (LI) in radix liquiritiae; chlorogenic acid (CG) and caffeic acid (CA) in folium artemisiae argyi; and verbascoside (VS) in radix rehmanniae, which were found to be responsible for the biological activities in the six single herbs and proved to be the active components . Their chemical structures are shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation and comparison of eight components in Jiao‐ai decoction and Si‐wu decoction by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry

Yao

Liu

et al. 2016

J of Separation Science

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An ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry method has been established to evaluate the variations of multiple components of Chinese herbal preparations, Jiao-ai decoction and Si-wu decoction, through the simultaneous determination of eight major active compounds with a huge difference in the level of content. Chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a mobile phase consisting of acetonitrile (0.1% formic acid) and water (0.1% formic acid) under gradient elution. A triple quadrupole tandem mass spectrometer was operated in positive and negative ionization modes, respectively, with multiple reaction monitoring for the detection of the eight compounds. All calibration curves showed excellent linear regressions (r > 0.99) within the test range. The precision, repeatability, and stability of the eight compounds were below 5.0% in terms of relative standard deviation. The recoveries were 97.0-102.4% with a relative standard deviation of 1.21-3.65% for all samples. In conclusion, a rapid, sensitive, precise, accurate, and reliable method has been developed for the simultaneous detection of eight active compounds in the pharmaceutical samples of Jiao-ai decoction and Si-wu decoction, which can be applied for the multicomponent comparison and further quality control.

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Evaluation of the Protective Effects of Chinese Herbs against Biomolecule Damage Induced by Peroxynitrite

Cited by 5 publications

References 25 publications

A novel analytical method based on HPLC-PDA coupled post-column derivatization to evaluate the ability to inhibit tyrosine nitration in lotus leaf extracts

A novel analytical method based on HPLC-PDA coupled post-column derivatization to evaluate the ability to inhibit tyrosine nitration in lotus leaf extracts

Biological activity of clovers – Free radical scavenging ability and antioxidant action of sixTrifoliumspecies

Simultaneous quantitation and comparison of eight components in Jiao‐ai decoction and Si‐wu decoction by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry

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