Evaluation of the Protective Effects of CoQ₁₀ on Ovarian I/R Injury: An Experimental Study

Abstract

Background: The aim of this study was to investigate the protective effects of coenzyme Q₁₀ (CoQ₁₀) on ovarian ischemia/reperfusion injury in an experimental rat adnexal torsion model. Methods: 48 female adult Wistar albino rats, weighing 220-250 g, were randomly equally divided into six groups (n = 8): sham, torsion, detorsion, sham+CoQ₁₀, torsion+CoQ₁₀, and detorsion+CoQ₁₀ groups. Bilateral adnexal torsion was performed for 3 h in all groups, except the sham and sham+CoQ₁₀ groups. Bilateral adnexal detorsion was performed on the detorsion and detorsion+CoQ₁₀ groups. CoQ₁₀ was injected intraperitoneally 30 min before the sham operation, torsion, and detorsion. Results: The torsion and detorsion groups had significantly higher histologic evaluation scores, as well as higher MDA levels, TOS values, and oxidative stress index values than the sham group. A strong correlation between total histologic evaluation scores for ischemia/reperfusion injury and the oxidative stress index was found. The mean oxidant marker levels and histopathologic scores for the ovarian tissue significantly decreased after using CoQ₁₀, which is a potent antioxidant. Conclusions: Conservative surgery (detorsion) was found to provide inadequate protection to ovarian tissue. The results of this study suggest that CoQ₁₀ could be useful for the protection of ovarian tissue before conservative surgery.

The Effect of Controlled Reperfusion in the Prevention of Infertility Caused by Ischemia Induced in the Contralateral Ovary in Rats with Unilateral Ovariectomy

“…Similar to our results, CR protected the rat ovaries against oxidative damage caused by I/R in the study by Ingec et al [11]. Experimental studies involving animals show that the I/R procedure causes severe oxidative damage in ovarian tissue [13,22]. Consistent with our study, Celik et al [23] reported an increase in oxidant parameters including MDA levels and myeloperoxidase activities (an indicator of neutrophil infiltration) and a decrease in antioxidant enzymes including GSH peroxidase and superoxide dismutase activities in ovarian tissue subjected to an I/R injury.…”

Is the Measurement of Serum Ischemia-Modified Albumin the Best Test to Diagnose Ovarian Torsion?

“…Ovarian torsion is a serious condition, and a delay in surgical intervention or even the use of some protective agents may result in the loss of the ovary [20,21,22]. Although our study reported promising findings that indicate the ability to find a serum marker to help triage patients with lower quadrant pain related to possible ovarian torsion, the risk of ovarian loss or other worse conditions makes this test of limited clinical utility at this time.…”

Sildenafil Reduces Ischemia-Reperfusion Injury in Rat Ovary: Biochemical and Histopathological Evaluation

“…Tissue damage is affected by the duration of I/R, and detorsioned tissue has local and systemic problems with respect to vitality and functional capacity. Various therapeutic agents have been tested to reduce tissue damage in the case of ovarian I/R injury [2,3,4,5]. However, ovarian function is maintained in the majority of cases (approximately 95%) after detorsion [1].…”