Evaluation of the preventive effect of phage cocktails on turbot ascites and its influence on main physiological indicators

Cui, Huijing; Xu, Yongping; Cong, Cong; Li, Caixia; Li, Xiaoyü; Li, Shuying; Ji-bin, LI; Wang, Lili

doi:10.1016/j.aquaculture.2021.737539

Cited by 7 publications

(4 citation statements)

References 46 publications

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“…61,147 There are a few studies reported that specific phages of E. tarda, E. İctaluri, and E. piscicida stop especially growth of bacteria and reduce edwardsiellosis in vitro in D. rerio, P. hypophthalmus, A. japonica and S. maximus. 94,[106][107][108][109][110][111][112][113] Various Vibrio species, such as V. harveyi, V. vulnificus, V. anguillarum, V. parahaemolyticus, V. alginolyticus, V. coralliilyticus, V. splendidus, and P. damselae (formerly V. damselae) are the cause of vibriosis have also been controlled by vibriophages which is biocontrol agents in fish (S. maximus L., S. salar, D. labrax, D. rerio, G. morhua L.) and P. monodon, L. vannamei, P. ornatus, H. laevigata, A. japonicus. 187 Studies have reported that approximately 60 bacteriophages were morphologically identified and genome sequenced and applied against Vibrio strains with no side effects shown in Table 2.…”

Section: Fpv4 and Fpsv-d22 Omykissmentioning

confidence: 99%

Bacteriophage applications in aquaculture

Çağatay

2023

Israeli Journal of Aquaculture - Bamidgeh

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Aquaculture has grown tremendously due to the big demand for its products. However, diseases affecting aquaculture and economic losses are worldwide problems and it needs low cost, sustainable, highly efficient, specific and eco-friendly therapeutants. Due to the rising up antibiotic resistant-microorganism, bacteriophage therapy has reinvigorated to replace antibiotics in agriculture, medicine, food safety and the environment. Likewise, it also holds great promise to avoid, control and treat bacteria in aquaculture to decrease the mortality level of different aquatic animal diseases. The isolation and characterization of new phages and phage application therapy to eliminate bacterial fish and shellfish pathogens such as Vibrio, Aeromonas, Pseudomonas, Lactococcus, Yersinia, Flavobacterium, and Streptococcus was gradually reported in aquaculture literature. The present review summarizes large-scale reports in vitro or in vivo use of aquaphage studies and applications in fish diseases from the 1980s to 2022 and future directions.

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Section: Fpv4 and Fpsv-d22 Omykissmentioning

confidence: 99%

Bacteriophage applications in aquaculture

Çağatay

2023

Israeli Journal of Aquaculture - Bamidgeh

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“…Available evidence suggests that administration of E. tarda phage vB_EtaM_ET-ABTNL-9 by feeding, injection, or immersion significantly reduced mortality as well as nonspecific immune-related enzyme activities of turbot Scophthalmus maximus 18 . Furthermore, a phage cocktail composed of E. tarda phage PETp9 and V. harveyi phage PVHp5 significantly reduced host levels of pathogens and maintained the normal gut microbiota profile for ascites prevention in turbot 19 . However, a comprehensive evaluation of the therapeutic efficacy and safety of phages for the biocontrol of edwardsiellosis caused by E. piscicida infections is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage EPP-1, a potential antibiotic alternative for controlling edwardsiellosis caused by Edwardsiella piscicida while mitigating drug-resistant gene dissemination

Han,

Huang,

Liu

et al. 2024

Sci Rep

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Edwardsiella piscicida causes significant economic losses to the aquaculture industry worldwide. Phage-based biocontrol methods are experiencing a renaissance because of the spread of drug-resistant genes and bacteria resulting from the heavy use of antibiotics. Here, we showed that the novel Edwardsiella phage EPP-1 could achieve comparable efficacy to florfenicol using a zebrafish model of Edwardsiella piscicida infection and could reduce the content of the floR resistance gene in zebrafish excreta. Specifically, phage EPP-1 inhibited bacterial growth in vitro and significantly improved the zebrafish survival rate in vivo (P = 0.0035), achieving an efficacy comparable to that of florfenicol (P = 0.2304). Notably, integrating the results of 16S rRNA sequencing, metagenomic sequencing, and qPCR, although the effects of phage EPP-1 converged with those of florfenicol in terms of the community composition and potential function of the zebrafish gut microbiota, it reduced the floR gene content in zebrafish excreta and aquaculture water. Overall, our study highlights the feasibility and safety of phage therapy for edwardsiellosis control, which has profound implications for the development of antibiotic alternatives to address the antibiotic crisis.

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“…In another study, it was demonstrated that feeding shieldfish with a phage cocktail led to a significant reduction in the numbers of Vibrio and Edwardsiella species in their intestines. Furthermore, phage cocktail feeding was reported to effectively prevent bacterial dysbiosis in shieldfish [23].…”

Section: Introductionmentioning

confidence: 99%

Partial Characterization of Three Bacteriophages Isolated from Aquaculture Hatchery Water and Their Potential in the Biocontrol of Vibrio spp.

Yaşa,

Evran,

Eren Eroğlu

et al. 2024

Microorganisms

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Bacteriophages are currently considered one of the most promising alternatives to antibiotics under the ‘One Health’ approach due to their ability to effectively combat bacterial infections. This study aimed to characterize Vibrio species in hatchery water samples collected from an aquaculture farm and investigate the biocontrol potential of their bacteriophages. Vibrio spp. (n = 32) isolates confirmed by LNA probe-based qPCR were used as hosts. Three Vibrio phages were isolated. IKEM_vK exhibited a broad host range, infecting V. harveyi (n = 8), V. alginolyticus (n = 2), V. azureus (n = 1), and V. ordalii (n = 1). IKEM_v5 showed lytic activity against V. anguillarum (n = 4) and V. ordalii (n = 1), while IKEM_v14 was specific to V. scophtalmi (n = 4). The morphological appearance of phages and their lytic effects on the host were visualized using scanning electron microscopy (SEM). All three phages remained relatively stable within the pH range of 6–11 and up to 60 °C. The lytic activities and biofilm inhibition capabilities of these phages against planktonic Vibrio cells support their potential applications in controlling vibriosis in aquaculture systems.

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Evaluation of the preventive effect of phage cocktails on turbot ascites and its influence on main physiological indicators

Cited by 7 publications

References 46 publications

Bacteriophage applications in aquaculture

Bacteriophage applications in aquaculture

Bacteriophage EPP-1, a potential antibiotic alternative for controlling edwardsiellosis caused by Edwardsiella piscicida while mitigating drug-resistant gene dissemination

Partial Characterization of Three Bacteriophages Isolated from Aquaculture Hatchery Water and Their Potential in the Biocontrol of Vibrio spp.

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