Evaluation of the prebiotic potential of arabinoxylans extracted from wheat distillers’ dried grains with solubles (DDGS) and in-process samples

Abstract

Distillers' dried grains with solubles (DDGS) is a low-value agro-industrial by-product, rich in arabinoxylans (AX), which is produced by commercial distillery and bioethanol plants. In a first approach, we investigated the prebiotic potential of four fractions comprising arabinoxylan oligosaccharides (AXOS) and xylooligosaccharides (XOS) obtained by enzymatic hydrolysis of AX fractions derived from DDGS and wet solids (in-process sample of DDGS production process). Anaerobic batch cultures in controlled pH conditions were used to test the prebiotic activity of the samples. Results did not show significant differences between the enzymatic treatments used, and all AXOS/XOS were extensively fermented after 24 h. In addition, significant increases (P < 0.05) in Bifidobacterium and total short-chain fatty acids (SCFAs) were observed after 24 h of fermentation. Finally, DDGS-derived hydrolysates were separated on an anionic semi-preparative column to prepare AXOS/XOS fractions with degree of polymerisation (DP) greater than 3. Bifidogenic activity and an increase of SCFAs were again observed after 24 h of fermentation, although this time, the selectivity was higher and the fermentation slower, suggesting that the fermentation of this substrate could take place (at least partially) in the distal part of the colon with highly desirable beneficial effects.

Low‐carbohydrate high‐fat weight reduction diet induces changes in human gut microbiota

“…Thus, the reduction of Bifidobacterium levels after WLD intervention can be attributed to the low intake of cereal grains and starchy vegetables. There is some evidence to support that bifidobacteria are supported by arabinoxylan oligosaccharides, although only a few studies show that long‐chain arabinoxylan is bifidogenic (Hopkins et al, 2003; Monteagudo‐Mera et al, 2018; Truchado et al, 2017). The growth of bifidobacteria is supported by dietary fructans (Dewulf et al, 2013), which are enriched in the wheat endosperm.…”

Shaping the Future of Probiotics and Prebiotics

“…The 1.3 billion tons of food waste generated annually in the food chain ii represents a rich and sustainable source of natural bioactive ingredients. Many side streams from fruit, vegetable, and grain processing contain potential prebiotics, such as pectin from orange peel [41] and arabinoxylans from distillery and brewing waste [42]. Future prebiotic compounds may also be chemically or structurally modified by the application of sonication, high pressure, acid, enzyme and oxidation treatments, in order to modify functionality.…”

Staphylococcus aureus FadB is a dehydrogenase that mediates cholate resistance and survival under human colonic conditions

“…An in vitro anaerobic batch culture system was used to simulate the main physiological and microbiological processes in the distal colon, including residence time, substrate availability and pH [45,46]. The experiment was carried out in triplicate using faecal samples from three healthy volunteers.…”