Evaluation of the potential of anoxic biodegradation of intracellular and dissolved microcystins in lake sediments

Wu, Xingqiang; Wang, Chunbo; Tian, Cuicui; Xiao, Baihua; Song, Lirong

doi:10.1016/j.jhazmat.2015.01.015

Cited by 20 publications

(17 citation statements)

References 36 publications

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“…The sediment sample from EHL contained the 2.40% total organic carbon and 0.27% total nitrogen. Its pH was 7.20 (Wu et al, 2015). Sediment samples were obtained by scraping the sediment surface (0–15 cm) with a 3-liter sampler.…”

Section: Methodsmentioning

confidence: 99%

Conductive Fe3O4 Nanoparticles Accelerate Syntrophic Methane Production from Butyrate Oxidation in Two Different Lake Sediments

Zhang

2016

Front. Microbiol.

151

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Syntrophic methanogenesis is an essential link in the global carbon cycle and a key bioprocess for the disposal of organic waste and production of biogas. Recent studies suggest direct interspecies electron transfer (DIET) is involved in electron exchange in methanogenesis occurring in paddy soils, anaerobic digesters, and specific co-cultures with Geobacter. In this study, we evaluate the possible involvement of DIET in the syntrophic oxidation of butyrate in the enrichments from two lake sediments (an urban lake and a natural lake). The results showed that the production of CH4 was significantly accelerated in the presence of conductive nanoscale Fe3O4 or carbon nanotubes in the sediment enrichments. Observations made with fluorescence in situ hybridization and scanning electron microscope indicated that microbial aggregates were formed in the enrichments. It appeared that the average cell-to-cell distance in aggregates in nanomaterial-amended enrichments was larger than that in aggregates in the non-amended control. These results suggested that DIET-mediated syntrophic methanogenesis could occur in the lake sediments in the presence of conductive materials. Microbial community analysis of the enrichments revealed that the genera of Syntrophomonas, Sulfurospirillum, Methanosarcina, and Methanoregula were responsible for syntrophic oxidation of butyrate in lake sediment samples. The mechanism for the conductive-material-facilitated DIET in butyrate syntrophy deserves further investigation.

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Section: Methodsmentioning

confidence: 99%

Conductive Fe3O4 Nanoparticles Accelerate Syntrophic Methane Production from Butyrate Oxidation in Two Different Lake Sediments

Zhang

2016

Front. Microbiol.

151

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“…Industrialization and urbanization have led to a substantial amount of industrial and domestic wastewater being discharged into natural water resources worldwide, resulting in increasingly serious levels of eutrophication . Cyanobacteria (also known as Cyanophyceae ) are photosynthetic organisms that can proliferate in marine and freshwater environments with high loads of nutrients (e. g., nitrogen and phosphorus compounds), attributable to anthropogenic enrichment . The rapid proliferation of cyanobacteria in marine and freshwater environments is of particular concern for human and ecosystem health because of the potential for bloom‐forming taxa to produce toxins.…”

Section: Introductionmentioning

confidence: 99%

“…MCs are a large group of cyclic heptapeptides, with more than 100 structural variants. MC‐LR contains Leucine amino acid and an arginine amino acid in variable positions, and it is the most common and toxic variant .…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

et al. 2018

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Microcystins are potent hepatotoxins produced by cyanobacteria, which proliferate in wastewaters with high nutrient content. Due to their high toxicity and potential risk to human health, even at low concentrations, the development of a sensitive and rapid method for the monitoring of microcystin‐LR (MC‐LR) in water samples is of great importance. In this context, a new direct electrochemical nano‐immunosensor for MC‐LR detection using the liquid crystal (E)‐1‐decyl‐4‐[(4‐decyloxyphenyl)diazenyl]pyridinium bromide (Br‐Py) as a redox probe and gold nanoparticles stabilized in bovine serum albumin (AuNP‐BSA) is described herein. The microcystin‐LR antibody (anti‐MC‐LR) was covalently immobilized using N‐(3‐dimethylaminopropyl)‐N‐ethylcarbodiimide hydrochloride (EDC) and N‐hydroxysuccinimide (NHS) on an AuNP‐BSA/BrPy film. The proposed sensor response is based on the inhibition of the Br‐Py electrochemical signal after the specific interaction of MC‐LR with immobilized anti‐MC‐LR on the electrode surface. The electrochemical behavior of the immunosensor was studied by square‐wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Under optimized conditions, using SWV and an incubation time of 15 min, the immunosensor exhibits a linear response to MC‐LR concentrations of 0.05 to 500.0 ng mL−1 with a detection limit of 0.05 ng mL−1. The anti‐MC‐LR/AuNP‐BSA/Br‐Py/GCE was successfully applied in the determination of MC‐LR in spiked seawater samples.

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“…There was no lag phase observed in the degradation processes of all groups, which reflected the extensive existence of MC-degrading bacteria in the original microbial community [31]. Wu et al found that dissolved MCs could be degraded by lake sediments after 2-6 days of lag phase under anoxic conditions [13]. The degradation capacity of a specific chemical by the microbial community can be considered as a relevant indicator of exposure to this pollutant in the natural environment [32].…”

Section: Discussionmentioning

confidence: 97%

Effects of Microcystin-LR on Metabolic Functions and Structure Succession of Sediment Bacterial Community under Anaerobic Conditions

Ding

Liu

Song

et al. 2020

Toxins

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Microcystins (MCs), which are produced by harmful cyanobacteria blooms, pose a serious threat to environmental health. However, the effect of MCs on the bacterial community under anaerobic conditions is still unclear. This study examined the dynamic changes of MC-degrading capacity, metabolic activity, and structure of the bacterial community in lake sediment repeatedly treated with 1 mg/L microcystin-LR (MC-LR) under anaerobic conditions. The results showed that the MC-degrading capacity of the bacterial community was increased nearly three-fold with increased treatment frequency. However, the metabolic profile behaved in exactly opposite trend, in which the overall carbon metabolic activity was inhibited by repeated toxin addition. Microbial diversity was suppressed by the first addition of MC-LR and then gradually recovered. The 16S amplicon sequencing showed that the dominant genera were changed from Exiguobacterium and Acinetobacter to Prosthecobacter, Dechloromonas, and Agrobacterium. Furthermore, the increase in the relative abundance of Dechloromonas, Pseudomonas, Hydrogenophaga, and Agrobacterium was positively correlated with the MC-LR treatment times. This indicates that they might be responsible for MC degradation under anaerobic conditions. Our findings reveal the relationship between MC-LR and the sediment bacterial community under anaerobic conditions and indicate that anaerobic biodegradation is an effective and promising method to remediate MCs pollution.

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Evaluation of the potential of anoxic biodegradation of intracellular and dissolved microcystins in lake sediments

Cited by 20 publications

References 36 publications

Conductive Fe3O4 Nanoparticles Accelerate Syntrophic Methane Production from Butyrate Oxidation in Two Different Lake Sediments

Conductive Fe3O4 Nanoparticles Accelerate Syntrophic Methane Production from Butyrate Oxidation in Two Different Lake Sediments

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

Effects of Microcystin-LR on Metabolic Functions and Structure Succession of Sediment Bacterial Community under Anaerobic Conditions

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