Evaluation of the physico-chemical properties of hydrocarbons-exposed bacterial biomass

Smułek, Wojciech; Zdarta, Agata; Grzywaczyk, Adam; Siwińska-Ciesielczyk, Katarzyna; Ciesielczyk, Filip; Strzemięcka, Beata; Jesionowski, Teofil; Voelkel, Adam; Kaczorek, Ewa

doi:10.1016/j.colsurfb.2020.111310

Cited by 4 publications

(5 citation statements)

References 49 publications

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“…The lowest cell adhesivity value was obtained for the OS.4 strain with NFT—51.44%. It is worth noting that in the case of this series of tests, the cell adhesivity value does not drop below 50%, which proves that the dye is incorporated into the structure of bacterial cells membrane (Smułek et al 2020 ). The addition of the extract caused a significant drop in the Congo Red adsorption, even below 2%.…”

Section: Resultsmentioning

confidence: 53%

“…To investigate deeper, the saponins-antibiotics co-action, the enzymatic activity of Pseudomonas spp. was assessed by MTT assay (Smułek et al 2020 ) and the results are presented in Fig. 3 .…”

Section: Resultsmentioning

confidence: 99%

“…The analysis of cell surface properties included evaluation of Congo red binding to microbial cells, according to Ambalam et al ( 2012 ). Moreover, the cell membrane permeability test using crystal violet was performed, as well as an MTT enzymatic activity test, as described by Smulek et al ( 2020 ). The zeta potential was calculated from the Smoluchowski equation after measurements of electrophoresis mobility using a Zetasizer Nano ZS instrument (Malvern Instruments Ltd. UK).…”

Section: Methodsmentioning

confidence: 99%

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Co-interaction of nitrofuran antibiotics and the saponin-rich extract on gram-negative bacteria and colon epithelial cells

Grzywaczyk

Smułek

Olejnik

et al. 2023

World J Microbiol Biotechnol

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Large-scale use of nitrofurans is associated with a number of risks related to a growing resistance to these compounds and the toxic effects following from their increasing presence in wastewater and the environment. The aim of the study was to investigate an impact of natural surfactant, saponins from Sapindus mukorossi, on antimicrobial properties of nitrofuran antibiotics. Measurements of bacterial metabolic activity indicated a synergistic bactericidal effect in samples with nitrofurantoin or furazolidone, to which saponins were added. Their addition led to more than 50% greater reduction in viable cells than in the samples without saponins. On the other hand, no toxic effect against human colon epithelial cell was observed. It was found that exposure to antibiotics and surfactants caused the cell membranes to be dominated by branched fatty acids. Moreover, the presence of saponins reduced the hydrophobicity of the cell surface making them almost completely hydrophilic. The results have confirmed a high affinity of saponins to the cells of Pseudomonas strains. Their beneficial synergistic effect on the action of antibiotics from the nitrofuran group was also demonstrated. This result opens promising prospects for the use of saponins from S. mukorossi as an adjuvant to reduce the emission of antibiotics into the environment.

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Co-interaction of nitrofuran antibiotics and the saponin-rich extract on gram-negative bacteria and colon epithelial cells

Grzywaczyk

Smułek

Olejnik

et al. 2023

World J Microbiol Biotechnol

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“…Hydrocarbons released in soil affect the biotic and abiotic components of soil. Therefore, it is essential to have vigorous effective measures for dealing with hydrocarbon contamination problems [4]. Bioremediation has emerged as the most auspicious treatment option for decontaminating polluted soil and water since its fruitful application to clean up the Exxon Valdez in 1989.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of Hydrocarbonoclastic Representative Bacteria

Oba,

John,

Garba

et al. 2022

JEP

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This study was designed and carried out to characterize hydrocarbonoclastic microbial communities in soil polluted with artisanal refined hydrocarbon at Trans Amadi, Phalga Local Government Area of Rivers State, Nigeria. Heterotrophic bacteria count ranged from 8.0 × 10 5 cfu/gm for sample TSAS1, and 2.1 × 10 6 cfu/gm for sample TSAS2 while TSAS3 was too numerous to count (TNTC). Hydrocarbon utilizing bacteria count ranged from 1.1 × 10 5 cfu/gm for TSAS1, and 5.9 × 10 4 cfu/gm for TSAS2, while TSAS3 was 5.4 × 10 4 cfu/gm. Physiochemical parameters of the soil were determined. The ranges obtained were pH 6.6, conductivity 125 μs/cm, temperature 27.3˚C, moisture 7.72, total nitrogen 0.056%, phosphate 1.554 ppm, potassium 145.87 ppm, lead 7.02 ppm, cadmium 0.41 ppm, nickel 1.96 ppm, copper 1.14 ppm, total petroleum hydrocarbon 1487.24181 ppm, polycyclic aromatic hydrocarbon 12.85287 ppm. Isolates of hydrocarbon utilizing bacteria characterized belonged to the genera Escherichia coli, Klebsiella sp., Lactobacillus sp., Enterobacter sp., Serratia sp., and Proteus sp. The findings in this study have revealed the abilities of these groups of bacteria to be employed in bioremediation/biodegradation clean-up practices. Thus the polluted soil may harbour important genera of bacterial species that may have beneficial applications in environmental microbiology for future remediation processes.

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“…To maintain optimal microorganism cultivation, growth media parameters such as pH, temperature, oxygen level, and nutrition agents must be ensured, depending on the species of microbe (Thomas, 2015 [ 1 ]). This causes the use of many different specific and non-specific growth media, such as general-purpose nutrient agar (Smułek et al, 2020 [ 2 ]) and enrichment broth (Rahman et al, 2020 [ 3 ]), specific for certain fungi Sabouraud’s agar (Cultrera et al, 2021 [ 4 ]), and Middlebrook and Löwenstein-Jensen media used for Mycobacterium spp. culture (Schön et al, 2020 [ 5 ]; Xie et al, 2021 [ 6 ]) (Mikolajczyk et al, 2016 [ 7 ]).…”

Section: Introductionmentioning

confidence: 99%

New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization

et al. 2021

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Biotechnological use of probiotic microorganisms involves providing them with appropriate conditions for growth, but also protection against environmental changes caused by an exchange of the medium, isolation of metabolites, etc. Therefore, the research on effective immobilization of probiotic microorganisms should be focused in this direction. The present study aimed to evaluate the effectiveness of an innovative hybrid immobilization system based on electrospun nanofibers and alginate hydrogel. The analyses carried out included the study of properties of the initial components, the evaluation of the degree and durability of cell immobilization in the final material, and their survival under stress conditions. Effective binding of microorganisms to the hydrogel and nanofibers was confirmed, and the collected results proved that the proposed biocomposite is an efficient method of cell protection. In addition, it was shown that immobilization on electrospun nanofibers leads to the preservation of the highest cell activity and the least cell growth restriction as compared to free or lyophilized cells only. The completed research opens new perspectives for the effective immobilization of microorganisms of significant economic importance.

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Evaluation of the physico-chemical properties of hydrocarbons-exposed bacterial biomass

Cited by 4 publications

References 49 publications

Co-interaction of nitrofuran antibiotics and the saponin-rich extract on gram-negative bacteria and colon epithelial cells

Co-interaction of nitrofuran antibiotics and the saponin-rich extract on gram-negative bacteria and colon epithelial cells

Bioprospecting of Hydrocarbonoclastic Representative Bacteria

New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization

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