Evaluation of the Phadebact Gonococcus Test in the identification of Neisseria gonorrhoeae in a routine diagnostic laboratory.

Abstract

SUmmARY The Phadebact Gonococcus Test, a coagglutination procedure for the confirmation of identity of presumptive N gonorrhoeae isolates, was evaluated under normal working conditions in a routine diagnostic laboratory and compared with an immunofluorescence technique. Of 166 isolates of N gonorrhoeae from urogenital, rectal, and pharyngeal sites, 164 gave a positive coagglutination reaction, and one of the two negative isolates gave a positive reaction on retesting after subculture. There were no cross-reactions with other organisms tested. This was in contrast with the immunofluorescence technique; with this method, three of 46 isolates of N meningitidis fluoresced brightly, and a further 17 isolates of N meningitidis gave reactions that were difficult to interpret.The coagglutination test is rapid, simple and a more specific alternative to immunofluorescence.

The characterization and pathological significance of gastric campylobacter-like organisms in the ferret: a model for chronic gastritis?

“…The tissues were homogenized in the transport broth in Griffiths tubes and resulting suspensions inoculated on heated blood agar and a selective medium (VCAT) used for the isolation of campylobacters from faeces and of Neisseria gonorrhoeae (VCAT medium: GC agar base (Oxoid) with 10% saponin-lysed horse blood, dextrose (1 g/l), L-glutamine (100 mg/l), L-cysteine (11 mg/l), cocarboxylase (1 mg/1), ferric nitrate (0-2 mg/l), vancomycin (3 mg/I), colistin (100000 units/l), amphotericin (1 mg/l), trimethoprim (5 mg/l)), (Tompkins et al 1981). Plates were incubated microaerobically (3 1 jar with Oxoid Gas Kit BR56) at 37°C for 5 days and examined for bacterial growth.…”