Evaluation of the PCR-Luminex method for four-digital level genotyping of the HLA-A, HLA-B and HLA-DRB1 genes in the Japanese population.

枝里, 吉川; 詞子, 宮原; 妙子, 成瀬; 和典, 島田; 史啓, 東; 啓高, 原; 英俊, 猪子

doi:10.12667/mhc.10.21

Cited by 2 publications

(2 citation statements)

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“…Indirect staining was completed by incubation of cells with goat anti-mouse IgG-fluorescein isothiocyanate (FITC) (Immunotech, Miami, FL). HLA allele types were also confirmed by the Luminex method using DNA isolated from the cryopreserved PBMCs as described (G & G Science, Fukushima, Japan) [Kikkawa et al, 2003].…”

Section: Hla Typing Of Pbmcsmentioning

confidence: 99%

Anti‐HTLV‐1 tax antibody and tax‐specific cytotoxic T lymphocyte are associated with a reduction in HTLV‐1 proviral load in asymptomatic carriers

Akimoto

Kozako

Sawada

et al. 2007

Journal of Medical Virology

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Previous studies have suggested that higher anti-human T-lymphotropic virus 1 (HTLV-1) antibody titer and lower anti-HTLV-1 Tax antibody reactivity are risk factors for adult T-cell leukemia/lymphoma. In the present study, we analyzed the relationships between these factors and clarified their significance. Forty-five carriers were examined for anti-HTLV-1 and anti-Tax antibody by ELISA. In addition, 43 of the 45 carriers with HLA-A*0201 and/or A*2402 were examined for frequency of Tax-specific cytotoxic T lymphocytes (CTLs) using HTLV-1/HLA tetramers, and 44 were examined for proviral load by real-time PCR. The relationships between these factors were analyzed statistically. The frequencies of Tax11-19 and Tax301-309-specific CTLs were significantly higher in the anti-Tax antibody-positive group as compared with the antibody-negative group (P = 0.002 and 0.033, respectively). Anti-HTLV-1 antibody titer had a positive correlation with proviral load (P = 0.019), whereas anti-Tax antibody did not show a significant correlation. Higher frequencies of both Tax11-19 and Tax301-309-specific CTLs are related to a reduction in proviral load (P = 0.017 and 0.015, respectively). Synergistic interactions of humoral and cellular immunity against Tax protein were demonstrated in HTLV-1 carriers. Tax-specific CTL may reduce HTLV-1 proviral load to prevent asymptomatic carriers from developing adult T-cell leukemia/lymphoma.

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Section: Hla Typing Of Pbmcsmentioning

confidence: 99%

Anti‐HTLV‐1 tax antibody and tax‐specific cytotoxic T lymphocyte are associated with a reduction in HTLV‐1 proviral load in asymptomatic carriers

Akimoto

Kozako

Sawada

et al. 2007

Journal of Medical Virology

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show abstract

“…The Luminex 100 laboratory testing system is a multiplexed particle-based flow-cytometric system that facilitates the development of multiplexed assays to measure many different reactions simultaneously in a small sample volume. The combined advantages of unsurpassed multiplexing capabilities, incorporation of multiple assay formats, rapid assay development, and reasonable cost have made this technique applicable both to research and to medical diagnostics [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Distribution of HLA alleles and haplotypes in Jinuo and Wa populations in Southwest China

Shi

Ogata

et al. 2008

Human Immunology

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Evaluation of the PCR-Luminex method for four-digital level genotyping of the HLA-A, HLA-B and HLA-DRB1 genes in the Japanese population.

Cited by 2 publications

References 1 publication

Anti‐HTLV‐1 tax antibody and tax‐specific cytotoxic T lymphocyte are associated with a reduction in HTLV‐1 proviral load in asymptomatic carriers

Anti‐HTLV‐1 tax antibody and tax‐specific cytotoxic T lymphocyte are associated with a reduction in HTLV‐1 proviral load in asymptomatic carriers

Distribution of HLA alleles and haplotypes in Jinuo and Wa populations in Southwest China

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