Evaluation of the p-nitro-alpha-acetylamino-beta-hydroxypropiophenone differential test for identification of Mycobacterium tuberculosis complex

Morgan, Margie; Doerr, Kelly A.; Hempel, H O; Goodman, Nelson; Roberts, Glenn D.

doi:10.1128/jcm.21.4.634-635.1985

Cited by 29 publications

(8 citation statements)

References 6 publications

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“…2002). The compound p ‐nitro‐alpha‐acetylamino‐beta‐hydroxypropiophenone is used in an in vitro growth test (termed the NAP test) to discriminate M. tuberculosis complex from other mycobacteria (Morgan et al. 1985; Rastogi et al.…”

Section: Introductionmentioning

confidence: 99%

Acetophenones with selective antimycobacterial activity

Rajabi

Courreges

Montoya

et al. 2005

Lett Appl Microbiol

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Aims: Mycobacteria are a serious cause of infections in humans, with limited treatment options, as no new antibiotics have been developed against mycobacteria since the 1960s. In this study, the antimycobacterial activity of a small library of acetophenone (AP) compounds was analysed. Methods and Results: Twenty-three AP derivatives were examined for activity against mycobacteria using a microbroth assay. The compounds were bacteriostatic, with the most effective (cyclohexylacetophenone and piperidinoacetophenone) having minimal inhibitory concentrations of 246 lM M. Active compounds tended to be more hydrophobic, and may work by alkylation of as yet undetermined intracellular target protein(s). Cytotoxicity against eukaryotic cells was also determined and appears to be unrelated to the bacteriostatic activity. Significance and Impact of the Study: AP may serve as a novel group of useful therapeutics against the mycobacteria.

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Section: Introductionmentioning

confidence: 99%

Acetophenones with selective antimycobacterial activity

Rajabi

Courreges

Montoya

et al. 2005

Lett Appl Microbiol

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“…Subsequently, over 6,000 strains of mycobacteria in liquid culture have been tested for their sensitivity to NAP, and correct assignment of isolates as either M. tuberculosis or MOTT based on NAP susceptibility has been over 99% (26). The validity of this assay has been documented (20,30,36) and supported by a decade of clinical application. More recently, NAP has been incorporated into the BACTEC system for differentiation between M. tuberculosis and MOTT among clinical isolates (21,35).…”

Section: Discussionmentioning

confidence: 99%

“…phAE40, however, has the ability to infect several species of mycobacteria (18,23); as a result, a "glowing" strain is not always M. tuberculosis. Since we have established that effective antimycobacterial agents can attenuate phAE40-induced photon production by the tubercle bacillus (23), we reasoned that the compound p-nitro-␣-acetylamino-␤-hydroxy propiophenone (NAP), which has selective inhibitory activity against members of the M. tuberculosis complex (15,16,20,26,27,30,36), can be used in conjunction with the luciferase reporter mycobacteriophage (LRP) assay to differentiate photon production by the tubercle bacillus from that by mycobacteria other than tuberculosis (MOTT).…”

mentioning

confidence: 99%

Specific identification of Mycobacterium tuberculosis with the luciferase reporter mycobacteriophage: use of p-nitro-alpha-acetylamino-beta-hydroxy propiophenone

et al. 1997

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We have previously described a luciferase reporter mycobacteriophage (LRP) assay that can detect Mycobacterium tuberculosis and characterize mycobacterial drug susceptibility patterns within 24 to 48 h in positive cultures. One drawback of this LRP protocol is the ability of the recombinant mycobacteriophage phAE40 to infect a variety of Mycobacterium species, thus limiting its specificity for the detection of M. tuberculosis. In this study, we have (i) explored the host range of phAE40, (ii) developed a modified LRP assay that exploits the selective inhibitory effect of the compound p-nitro-␣-acetylamino-␤-hydroxy propiophenone (NAP) against members of the M. tuberculosis complex to differentiate between the tubercle bacillus and other mycobacterial species, and (iii) tested over 300 samples, including primary clinical isolates and drug-resistant strains of M. tuberculosis, demonstrating the ability of the NAP-modified LRP assay to identify M. tuberculosis complex organisms with high degrees of sensitivity and specificity. MATERIALS AND METHODS Reagents. All chemicals were obtained from Sigma Chemical (St. Louis, Mo.) unless otherwise indicated. NAP (kindly provided by Salman Siddiqi, Becton

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“…For identification of MTB or NTM, NAP ( p-nitro-alfa-acetylamino-beta-hydroxypropiophenone ) test was performed [ 25 , 26 ]. This test is highly specific for the identification of Mycobacterium tuberculosis complex (99%) [ 27 ]. In cases which NAP test could not be done, the identification of species were made by the visualization of the aspect of the colony – presence or absence of cord formation in liquid media – due to the high sensitivity and specificity of this method [ 28 , 29 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative radiological features of disseminated disease due to Mycobacterium tuberculosis vs non-tuberculosismycobacteria among AIDS patients in Brazil

et al. 2008

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BackgroundDisseminated mycobacterial disease is an important cause of morbidity and mortality in patients with HIV-infection. Nonspecific clinical presentation makes the diagnosis difficult and sometimes neglected.MethodsWe conducted a retrospective cohort study to compare the presentation of disseminated Mycobacterial tuberculosis (MTB) and non-tuberculous Mycobacterial (NTM) disease in HIV-positive patients from 1996 to 2006 in Brazil.ResultsTuberculosis (TB) was diagnosed in 65 patients (67.7%) and NTM in 31 (32.3%) patients. Patients with NTM had lower CD4 T cells counts (median 13.0 cells/mm3 versus 42.0 cells/mm3, P = 0.002). Patients with tuberculosis had significantly more positive acid-fast smears (48.0% vs 13.6%, P = 0.01). On chest X-ray, miliary infiltrate was only seen in patients with MTB (28.1% vs. 0.0%, P = 0.01). Pleural effusion was more common in patients with MTB (45.6% vs. 13.0%, P = 0.01). Abdominal adenopathy (73.1% vs. 33.3%, P = 0.003) and splenic hypoechoic nodules (38.5% vs. 0.0%, P = 0.002) were more common in patients with TB.ConclusionMiliary pulmonary pattern on X-ray, pleural effusion, abdominal adenopathy, and splenic hypoechoic nodules were imaging findings associated with the diagnosis of tuberculosis in HIV-infected patients. Recognition of these imaging features will help to distinguish TB from NTM in AIDS patients with fever of unknown origin due to disseminated mycobacterial disease.

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Evaluation of the p-nitro-alpha-acetylamino-beta-hydroxypropiophenone differential test for identification of Mycobacterium tuberculosis complex

Abstract: Thtep-nitro-a-acetylamino-pi-hydroxypropiophenone (NAP) differential test for the identification ofMycobacterium tuberculosis recovered from clinical specimens was evaluated by two laboratories and found to be a rapid and accurate procedure with a specificity exceeding 99%.

Cited by 29 publications

References 6 publications

Acetophenones with selective antimycobacterial activity

Acetophenones with selective antimycobacterial activity

Specific identification of Mycobacterium tuberculosis with the luciferase reporter mycobacteriophage: use of p-nitro-alpha-acetylamino-beta-hydroxy propiophenone

Comparative radiological features of disseminated disease due to Mycobacterium tuberculosis vs non-tuberculosismycobacteria among AIDS patients in Brazil

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