Evaluation of the osteogenesis and osseointegration of titanium alloys coated with graphene: an in vivo study

Li, Kewen; Wang, Chunhui; Yan, Jining; Zhang, Qi; Dang, Baoping; Wang, Zhuo; Yao, Yun; Lin, Kaifeng; Guo, Zhong-Shang; Bi, Long; Han, Yisheng

doi:10.1038/s41598-018-19742-y

Cited by 57 publications

(47 citation statements)

References 51 publications

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“…21,22 On the basis of GO, it is possible for carboxyl, 23 amino, 24 hydroxyl 25 and other functional groups to be introduced not only to improve the fluidity and dispersion of graphene, but also to further endow it with new functions, among which is that various active substances can be loaded to improve bioactivities. 26,27 At present, the functionalized GDs have been compounded with various substrates such as metals, 28 inorganic, 29 natural polymer 30 and synthetic polymer 31 to prepare scaffolds, 32,33 coatings, 34 membranes 35 and injectable hydrogels. 36 Lots of studies in vitro and in vivo have demonstrated that the GDscontaining composites could regulate the extracellular microenvironment to effectively promote bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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Section: Introductionmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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“…Osteolysis evaluated by X-ray was not observed in both the smooth and DED groups, but micro-CT analysis is necessary to make an accurate evaluation [36]. However, due to the metal interference effect of CoCr alloy and titanium, an accurate image of the bone-implant contact surface could not be obtained.…”

Section: Discussionmentioning

confidence: 96%

Osteo-Compatibility of 3D Titanium Porous Coating Applied by Direct Energy Deposition (DED) for a Cementless Total Knee Arthroplasty Implant: In Vitro and In Vivo Study

Ryu

Ban

Jung

et al. 2020

JCM

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Direct energy deposition (DED) technology has gained increasing attention as a new implant surface technology that replicates the porous structure of natural bones facilitating osteoblast colonization and bone ingrowth. However, concerns have arisen over osteolysis or chronic inflammation that could be caused by Cobalt-chrome (CoCr) alloy and Titanium (Ti) nanoparticles produced during the fabrication process. Here, we evaluated whether a DED Ti-coated on CoCr alloy could improve osteoblast colonization and osseointegration in vitro and in vivo without causing any significant side effects. Three types of implant CoCr surfaces (smooth, sand-blasted and DED Ti-coated) were tested and compared. Three cell proliferation markers and six inflammatory cytokine markers were measured using SaOS2 osteoblast cells. Subsequently, X-ray and bone histomorphometric analyses were performed after implantation into rabbit femur. There were no differences between the DED group and positive control in cytokine assays. However, in the 5-bromo-2′-deoxyuridine (BrdU) assay the DED group exhibited even higher values than the positive control. For bone histomorphometry, DED was significantly superior within the 1000 µm bone area. The results suggest that DED Ti-coated metal printing does not affect the osteoblast viability or impair osseointegration in vitro and in vivo. Thus, this technology is biocompatible for coating the surfaces of cementless total knee arthroplasty (TKA) implants.

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“…This may result from better adhesion strength between graphene-coatings and Ti substrates, which helped to maintain the integrity of graphene-coatings even under sustained mechanical interferences from in vivo environment. Clearly, this conclusion is limited to the animal models employed in our study, and graphene-coated titanium bone implants and in situ osteointegration 62 will be a practical in vivo model to illustrate the future application of graphene-coated Ti implants. Basing on the methods to enhance and evaluate the adhesion strength of graphene-coatings on Ti as proposed in this study, we will uncover the exact effect of bone integration of graphene-coated titanium bone implants in vivo by using the in situ osteointegration model in our future experiments.…”

Section: Discussionmentioning

confidence: 99%

Effects of thermal treatment on the adhesion strength and osteoinductive activity of single-layer graphene sheets on titanium substrates

et al. 2018

Sci Rep

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In recent years, dental implants have become the preferred approach for the restoration of missing teeth. At present, most dental implants are made of pure titanium, and are affected by peri-implantitis and bone resorption, which usually start from the implant neck, due to the complex environment in this region. To address these issues, in this study we modified the surface of titanium (Ti) implants to exploit the antibacterial and osteoinductive effects of single-layer graphene sheets. Chemical vapor deposition (CVD)-grown single-layer graphene sheets were transferred to titanium discs, and a method for improving the adhesion strength of graphene on Ti was developed due to compromised adhesion strength between graphene and titanium surface. A thermal treatment of 2 h at 160 °C was found to enhance the adhesion strength of graphene on Ti to facilitate clinical transformation. Graphene coatings of Ti enhanced cell adhesion and osteogenic differentiation, and imparted antibacterial activity to Ti substrate; these favorable effects were not affected by the thermal treatment. In summary, the present study elucidated the effects of a thermal treatment on the adhesion strength and osteoinductive activity of single-layer graphene sheets on titanium substrates.

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Evaluation of the osteogenesis and osseointegration of titanium alloys coated with graphene: an in vivo study

Cited by 57 publications

References 51 publications

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Osteo-Compatibility of 3D Titanium Porous Coating Applied by Direct Energy Deposition (DED) for a Cementless Total Knee Arthroplasty Implant: In Vitro and In Vivo Study

Effects of thermal treatment on the adhesion strength and osteoinductive activity of single-layer graphene sheets on titanium substrates

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