Evaluation of the Ophthalmotoxic Effect of Quantum Dots InP/ZnSe/ZnS 660 and Bioconjugates Based on Them in Terms of the Prospects for the Treatment of Resistant Endophthalmitis. Experimental Research. Part 2 (Stage 1)

Abstract

The problem of chemo/antibiotic resistance in modern medicine remains relevant today. The sensitivity of microorganisms (MO) determines the range of drugs used, which ultimately affects the effectiveness of treatment and the prognosis for the patient. However, taking into account the adaptation process of individual strains of MO, the uncontrolled use of antibiotics will inevitably lead to the maintenance of the so-called crisis of antibiotic resistance throughout the world, as well as the formation of a vicious circle that reduces the functional and anatomical outcomes of the treatment of any inflammatory diseases, including ophthalmological ones. This article presents the process of experimental creation and certification, assessment of the physicochemical properties of quantum dots, as well as biological nanoconjugates as an option for overcoming the antibiotic resistance of certain strains of microorganisms in the treatment of infectious and inflammatory pathology in ophthalmology, in particular endophthalmitis. Also, an animal model has demonstrated the safety of using InP / ZnSe / ZnS 660 quantum dot solutions for intravitreal administration in pure form and in combination with antibiotics.

OculusGraphy: Signal Analysis of the Electroretinogram in a Rabbit Model of Endophthalmitis Using Discrete and Continuous Wavelet Transforms

“…Twelve male New Zealand rabbits weighing between 3.0 to 3.2 kg (mean ± SD) 3.10 ± 0.35 kg and aged 2.0 to 3.5 months were treated for bacterial endophthalmitis of the right eye associated with staphylococcus aureus infection. The right eye of each rabbit was injected intravitreally with a solution of quantum dots 0.01% dose of QD InP/ZnSe/ZnS 660 in combination with vancomycin 10 mg/mL as a 0.1 mL solution [ 60 ]. Article [ 60 ] provides an exploration of the evaluation of ophthalmotoxicity stemming from quantum-dot-based bioconjugates pertaining to their potential in the treatment of resistant endophthalmitis.…”

Evaluation of the Anti-Infectious Activity of the Complex Based on Quantum Dots InP / ZnSe / ZnS 650 and Tobramycin Against <i>Pseudomonas Aeruginosa</i> Infection of the Cornea. Experimental Research

“…Ельцина», г. Екатеринбург. Оборудование и методики проведения измерений оптического поглощения (ОП) и фотолюминесценции (ФЛ) растворов подробно описаны в опубликованных ранее работах [12,13].…”

“…Отметим, что при возбуждении в диапазоне 400-500 нм наблюдается только люминесценция КТ в исследуемом комплексе КТ/Т (кривые 4-6). Полученные данные согласуются с выполненной ранее аттестацией абсорбционных и люминесцентных свойств КТ InP/ZnSe/ZnS 660 [13].…”

“…КТ, являясь универсальными донорами неспаренных электронов под действием источника возбуждения, способны к генерации различных типов внутриклеточных супероксидных радикалов внутри бактериальной клетки. Нанометровый размер кристалла КТ, а также моделируемые оптические и электронные свойства позволяют использовать их отдельно или в составе комплексов с существующими антибактериальными агентами для получения аддитивного и синергетического эффекта [7][8][9][10][11][12][13][14].…”

OculusGraphy: Comparison Between Human and Rabbit Electroretinogram Based on Time-Domain and Time-Frequency Domain Analysis

“…12 New Zealand rabbits (male), weighing from 3.0 to 3.2 kg (average 3.10 ± 0.35 kg), aged 2 to 3.5 months had treatment for bacterial endophthalmitis of the right eye associated with staphylococcus aureus. Each rabbit was injected with a solution of quantum dots 0.01% dose of QD InP/ZnSe/ZnS 660 in combination with Vancomycin 10 mg/ml [50]. In all cases, the volume of substance for intraocular administration was 0.1 ml (0.05 + 0.05 ml.).…”