Evaluation of the Neurogenic Potential in the Rat Inferior Colliculus from Early Postnatal Days Until Adulthood

Engert, Jonas; Rak, Kristen; Bieniussa, L; Scholl, Miriam; Hagen, Rudolf; Voelker, Johannes

doi:10.1007/s12035-020-02151-6

Cited by 6 publications

(14 citation statements)

References 65 publications

(90 reference statements)

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“…In adult rodents, NSCs have been widely documented in the SVZ and the SGZ [ 21 , 22 ], but the hypothalamus, the neocortex and the inferior colliculus have recently been identified as new potential neurogenic zones [ 23 , 24 , 25 , 26 ]. In these neurogenic regions, quiescent and activated NSCs are present, generating new neurons throughout life [ 25 , 26 , 27 ]. To highlight the potential neurogenic niche status of the vestibular nuclei in physiological conditions, we used a combination of cell markers to identify NSCs.…”

Section: Discussionmentioning

confidence: 99%

Vestibular Nuclei: A New Neural Stem Cell Niche?

Rastoldo

Watabe

Lapotre

et al. 2022

Cells

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We previously reported adult reactive neurogliogenesis in the deafferented vestibular nuclei following unilateral vestibular neurectomy (UVN) in the feline and the rodent model. Recently, we demonstrated that UVN induced a significant increase in a population of cells colocalizing the transcription factor sex determining region Y-box 2 (SOX2) and the glial fibrillary acidic protein (GFAP) three days after the lesion in the deafferented medial vestibular nucleus. These two markers expressed on the same cell population could indicate the presence of lesion-reactive multipotent neural stem cells in the vestibular nuclei. The aim of our study was to provide insight into the potential neurogenic niche status of the vestibular nuclei in physiological conditions by using specific markers of stem cells (Nestin, SOX2, GFAP), cell proliferation (BrdU) and neuronal differentiation (NeuN). The present study confirmed the presence of quiescent and activated adult neural stem cells generating some new neurons in the vestibular nuclei of control rats. These unique features provide evidence that the vestibular nuclei represent a novel NSC site for the generation of neurons and/or glia in the adult rodent under physiological conditions.

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Section: Discussionmentioning

confidence: 99%

Vestibular Nuclei: A New Neural Stem Cell Niche?

Rastoldo

Watabe

Lapotre

et al. 2022

Cells

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“…Interestingly, significantly lower expression of Sox-2 was present in the single-cell studies of the IC. In contrast, very similar expressions of the two markers were detected in neurospheres of the IC of PND 6 rats [ 41 ]. PND 8 animals were not evaluated in this study, but there was already a significant decrease in ATOH1 compared with PND 12 animals.…”

Section: Discussionmentioning

confidence: 99%

“…The IC showed 5.06 ± 1.12% astrocytic cells. Nestin-positive cells had a very similar expression profile of 13.24 ± 6.07% [ 41 ]. Interestingly, the MGB assumes a critical role in tinnitus pathology, and GFAP expression has been shown to remain stable in the rat tinnitus model compared to the auditory cortex [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Neurogenic Stem Cell Niche in the Auditory Thalamus: In Vitro Evidence of Neural Stem Cells in the Rat Medial Geniculate Body

Engert

Spahn

Bieniussa

et al. 2023

Life

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The medial geniculate body (MGB) is a nucleus of the diencephalon representing a relevant segment of the auditory pathway and is part of the metathalamus. It receives afferent information via the inferior brachium of the inferior colliculus and transmits efferent fibers via acoustic radiations to the auditory cortex. Neural stem cells (NSCs) have been detected in certain areas along the auditory pathway. They are of great importance as the induction of an adult stem cell niche might open a regenerative approach to a causal treatment of hearing disorders. Up to now, the existence of NSCs in the MGB has not been determined. Therefore, this study investigated whether the MGB has a neural stem cell potential. For this purpose, cells were extracted from the MGB of PND 8 Sprague-Dawley rats and cultured in a free-floating cell culture assay, which showed mitotic activity and positive staining for stem cell and progenitor markers. In differentiation assays, the markers β-III-tubulin, GFAP, and MBP demonstrated the capacity of single cells to differentiate into neuronal and glial cells. In conclusion, cells from the MGB exhibited the cardinal features of NSCs: self-renewal, the formation of progenitor cells, and differentiation into all neuronal lineage cells. These findings may contribute to a better understanding of the development of the auditory pathway.

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“…Immuno uorescence staining showed that the differential expression of Pnky did not affect the stem fate of C17.2 and NE4C, with the percentage of Nestin-positive cells up to 99% in each stable cell lines (Fig. 1b and f), which indicating the undifferentiated state of NSCs (Engert et al 2021). Further investigations of Pnky on cell proliferation were also carried out using cell counting and EdU assays.…”

Section: Lncrna Pnky Negatively Regulates the Proliferation Of Nscsmentioning

confidence: 92%

LncRNA Pnky Positively Regulates Neural Stem Cell Migration via Modulating mRNA Splicing and Export of Target Genes

et al. 2021

Preprint

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Directed migration of neural stem cells (NSCs) is critical for embryonic neurogenesis and the healing of neurological injuries. LncRNA Pnky was reported to regulate neuronal differentiation of NSCs. However, its regulatory effect on NSC migration has never been explored. Herein, we identified that Pnky was also a key regulator of NSC migration, as underscored by the fact that Pnky silencing restrained the migration of both NSC lines C17.2 and NE4C, whereas Pnky over-expression promoted their migration. Meanwhile, Pnky regulated the expression of a core set of critical regulators that directing NSC migration, such as MMP2, MMP9, AKT and p38MAPK, etc. Through preliminary bioinformatics, we surprisingly noticed that splicing factors U2AF1 and U2AF1L4, as well as mRNA export adaptors SARNP, Aly/Ref and THOC7, were predicted to strongly interact with Pnky. Mechanically, Pnky could co-localize and directly bind to U2AF1, SARNP, Aly/Ref and THOC7, indicating the involvement of Pnky in modulating mRNA splicing and export processes. Collectively, our data delineated that, through interacting with U2AF1, SARNP, Aly/Ref and THOC7, Pnky coupled and modulated the mRNA splicing and export of downstream factors, which consequently affected NSC migration. These findings provide a possible theoretical basis of NSC migration for brain development and damage repair.

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Evaluation of the Neurogenic Potential in the Rat Inferior Colliculus from Early Postnatal Days Until Adulthood

Cited by 6 publications

References 65 publications

Vestibular Nuclei: A New Neural Stem Cell Niche?

Vestibular Nuclei: A New Neural Stem Cell Niche?

Neurogenic Stem Cell Niche in the Auditory Thalamus: In Vitro Evidence of Neural Stem Cells in the Rat Medial Geniculate Body

LncRNA Pnky Positively Regulates Neural Stem Cell Migration via Modulating mRNA Splicing and Export of Target Genes

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