Evaluation of the Mytilus galloprovincialis Lam. digestive gland metallothionein as a biomarker in a long-term field study: Seasonal and spatial variability

Ivanković, Dušica; Pavičić, Jasenka; Erk, Marijana; Filipović-Marijić, V.; Raspor, Biserka

doi:10.1016/j.marpolbul.2005.04.039

Cited by 105 publications

(39 citation statements)

References 36 publications

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“…Discharge of untreated domestic wastewater presents an additional food source at Vranjic. In consistence with well documented relationship between nutrient load and feeding physiology in bivalves (Scholten and Smaal 1999), mussels from Vranjic were reported to have approximately 35% higher maximal condition indices in autumn seasons in the period from 1999 to 2001 compared to mussels from Inavinil (Ivanković et al 2005). Similar results were also obtained in our study in the last two sampling campaigns, with condition indices on average 30% higher at Vranjic (average CI: 19.1%) than Inavinil (average CI: 14.9%).…”

Section: Total Cytosolic Proteinssupporting

confidence: 53%

“…Analysis of site-specific differences performed on whole data set indicated overall highest MTwm (0.470 mg g -1 ), TP (16.33 mg g -1 ) and Zn level (5.42 g g -1 ) at Vranjic (Table 4). According to Ivanković et al (2005), as well as the preliminary report of the Croatian national monitoring programme Adriatic for the period from 1999 to 2001, Vranjic can be characterized as moderately eutrophycated coastal location with Dragun, Zrinka; Erk, Marijana; Ivanković, Dušica; Žaja, Roko; Filipović Marijić, Vlatka; Raspor, Biserka. Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue. // Environmental Science and Pollution Research.…”

Section: Total Cytosolic Proteinsmentioning

confidence: 99%

“…Other factors can contribute to MT variability, such as organism handling, starvation, anoxia, and the presence of antibiotics, vitamins or herbicides (Amiard et al 2006). The influence of food abundance, reproductive cycle, and seasonal and spatial factors on the MT levels have been reported for marine bivalves (Serafim et al, 2002;Raspor et al 2004;Ivanković et al, 2005). Due to a number of so far identified confounding factors (Amiard et al 2006), different MT levels in organisms from distinct locations may erroneously be interpreted as a consequence of anthropogenic activities.…”

Section: Introductionmentioning

confidence: 99%

“…The sampling sites within Kaštela Bay, Vranjic (VR) and Inavinil (IV), were classified as the sites occasionally influenced by freshwater (Ivanković et al 2005): Vranjic from small river Jadro and Inavinil from freshwater source situated next to the ex-chlor-alkali plant (active in the period from 1950 to 1990). However, despite the existing sources of contamination in the Kaštela Bay, the study conducted in 1997/1998 with transplanted mussels indicated that metal concentrations in mussel tissues, and especially in the gills, still did not reflect the anthropogenic influence; total and cytosolic metal levels in mussel tissues were uniform throughout the Bay, in contrast to pronounced variations of metal levels in the sediment (Odžak et al 2001;Dragun et al 2004).…”

mentioning

confidence: 99%

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Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Dragun

Erk

Ivanković

et al. 2009

Environ Sci Pollut Res

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Dragun, Zrinka; Erk, Marijana; Ivanković, Dušica; Žaja, Roko; Filipović Marijić, Vlatka; Raspor, Biserka. Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue. // Environmental Science and Pollution Research. 17 (2010) , 4; 977-986 Dragun, Zrinka; Erk, Marijana; Ivanković, Dušica; Žaja, Roko; Filipović Marijić, Vlatka; Raspor, Biserka. Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue. // Environmental Science and Pollution Research. 17 (2010) , 4; 977-986. 2 Abstract Purpose: The aim of this study was to compare the level of metal contamination in two bays in the middle part of the Eastern Adriatic coastal zone in Croatia using the gills of mussels Mytilus galloprovincialis as indicator tissue. Despite the existing sources of contamination, previous studies with caged mussels only indicated moderate metal contamination of the Kaštela Bay, contrary to the Trogir Bay in which marina and shipyard present a probable source of Cu-and Zn-contamination.Methods: The measurements of metallothioneins (MTs) and metals that induce MT synthesis (Cu, Zn and Cd) were performed in the heat-treated gill cytosol, and total proteins (TPs) in the untreated gill cytosol. MTs were determined by differential pulse voltammetry, Cu and Zn by flame atomic absorption spectrometry (AAS), Cd by graphite furnace AAS and TPs by Bradford spectrophotometric procedure.Results: The results collected in four sampling campaigns (autumn periods from [2001][2002][2003][2004] indicated that MT levels in mussel gills (expressed on dry mass basis 2.30.3 mg g -1 ) were comparable with basal levels reported in the literature (2.50.8 mg g -1 ). Observed interindividual, temporal and spatial MT variability could be associated with different confounding factors, such as the time of sampling, total protein concentration and mussel size rather than cytosolic levels of Cu and Zn. Metal levels, expressed on wet mass basis, in the heat-treated gill cytosol ranged from 1.33-11.31 g g -1 for Zn, from 0.72-2.96 g g -1 for Cu and from 0.036-0.100 g g -1 for Cd.The highest Zn level was measured at Vranjic (Kaštela Bay) -the site influenced by untreated domestic wastewater, while somewhat increased Zn and the highest Cu levels were found at marina and shipyard locations (Trogir Bay). The highest Cd level was measured at Inavinil (Kaštela Bay).Conclusions: The observed association of gill MT levels with several biotic and abiotic factors, limits its use as the biomarker of low-level metal exposure. Therefore, the use of the metal concentrations in the heat-treated gill cytosol of Mediterranean mussels should be considered for the assessment of the low-level metal contamination of coastal marine areas.

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Section: Total Cytosolic Proteinssupporting

confidence: 53%

Section: Total Cytosolic Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Dragun

Erk

Ivanković

et al. 2009

Environ Sci Pollut Res

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“…Various abiotic and biotic factors also can also affect the metal content in shellfish tissue (Phillips and Rainbow 1994;Ivanković et al 2005). In shellfish, some metals, such as, for example Ag and Cd, show an increase in concentration with age (Cain and Luoma 1990; Raspor et al 2004).…”

Section: Accumulation Of Heavy Metals In Soft Tissue Of Noah's Ark Shmentioning

confidence: 99%

Trace Metals in Noah’s Ark Shells (Arca noae Linnaeus, 1758): Impact of Tourist Season and Human Health Risk

Ivanković

Erk

Župan

et al. 2016

Arch Environ Contam Toxicol

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Commercially important bivalve Noah's Ark shell (Arca noae Linnaeus, 1758) represents a high-quality seafood product, but the data on levels of metal contaminants that could pose a human health risk and also on some essential elements that are important for health protection are lacking. This study examineds the concentrations of Cd, Pb, Cr, Ni, Cu, Co, and Zn in the mg/kg wet weight. These levels were lower than the permissible limits for safe consummation of seafood, and only for Cd, some precautions should be taken into account if older shellfish were consumed. Increase of Cd, Cr, and Cu in shell tissue was observed during the tourist season at the site closest to the marine traffic routes, indicating that metal levels in shellfish tissue should be monitored especially carefully monitored during the peak tourist season, to prevent eventual toxic effects due to increased intake of metals, specifically of Cd.

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Mussels and Marine Environmental Contaminants

2021

Marine Mussels

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Evaluation of the Mytilus galloprovincialis Lam. digestive gland metallothionein as a biomarker in a long-term field study: Seasonal and spatial variability

Cited by 105 publications

References 36 publications

Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Trace Metals in Noah’s Ark Shells (Arca noae Linnaeus, 1758): Impact of Tourist Season and Human Health Risk

Mussels and Marine Environmental Contaminants

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