Evaluation of the Mutagenic Properties of Two Lignans from Acanthopanax koreanum Nakai

Abstract

Acanthopanax koreanum Nakai, a well known traditional herb grown in Jeju Island, South of Korea, has been used as a tonic and sedative agent, as well as in the treatment of diabetes and immune diseases. Mutagenicity of two lignans, syringaresinol and tortoside A isolated from A. koreanum, was assessed using Salmonella/microsome (Ames) test. Tester strains used were Salmonella typhimurium TA98, TA100, TA1535, and Escherichia coli WP2uvrA. The mutagenic activity was determined both in the absence or presence of S9 mixture. As a result, tortoside A did not cause any increase in the number of his+ revertants in S. typhimurium and E. coli WP2uvrA strains in the presence or absence of S9 mix, compared to the controls. Similarly, low concentrations of syringaresinol (750 and 1,500 μg/plate) did not show any mutagenic properties in all bacterial strains, in the presence or absence of S9 mixture. However, in the high concentration of syringaresinol (3,000 μg/plate), the number of revertants were increased in TA1535 strains, in the absence of S9 metabolic activation. Therefore, in vivo experiments such as comet assay are needed to further determine the genotoxic/carciogenic potential of syringaresinol isolated from A. koreanum.

Safety and efficacy of a feed additive consisting of a tincture derived from the roots of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (taiga root tincture) for use in dogs, cats and horses (FEFANA asbl)

“…These findings are in line with the negative results obtained in Ames and comet assays with syringaresinol or its metabolites (Hong and Lyu, 2013;Kirsch et al, 2020).…”

Safety evaluation of mutagenicity, genotoxicity, and cytotoxicity of Lactobacillus spp. isolates as probiotic candidates

“…coli testers with and without S9 metabolic activation. Ames test could detect indirect mutants in the presence of S9 metabolic activation; however, in the absence of S9 metabolic activation, it could only detect direct mutants 26 . Studies on these mutations have shown that in cases where the mutagen is directly involved, it interacts directly with DNA and their reactive metabolites 27 …”