Evaluation of the mutagenic effects of SV40 in mouse, hamster, and mouse-human hybrid cells

Abstract

We have examined the ability of SV40 to induce changes in drug or temperature resistance in mouse, hamster, and mouse-human hybrid cells. SV40 induced a substantial increase of cells resistant to 5-bromodeoxyuridine + trifluorothymidine in Balb/c 3T3 cells and induced an increase of hybrid cells resistant to 6-thioguanine. SV40 was found to be nonmutagenic or weakly mutagenic in other test systems. The 3T3 cells were T-antigen positive, exhibited a marked reduction in TK activity, were heterogeneous for [3H]BrdU incorporation by autoradiography, and exhibited instability of the drug-resistance phenotype, suggesting that SV40 may be inducing resistance by an epigenetic process. SV40-induced 6-thioguanine resistance in the hybrids appears to occur predominantly by chromosome loss.

The Terminator mouse is a diphtheria toxin–receptor knock-in mouse strain for rapid and efficient enrichment of desired cell lineages

“…or selection (MDCK cells, e.g. ); and 2) continuous cell culture typically leads to significant epigenetic and phenotypic changes and the dysregulated expression of many genes when compared to the in vivo expression profile of the parent cell type 1, 2, 3 .…”

The terminator mouse: salvation for primary cell culture

“…However, immortalization influenced the phenotype and differentiation of the respective cells in culture to some extent. 7 More recently, a rapidly increasing number of genetically modified mice have become available to target specific cell populations within the kidney. Specific promoter/enhancer elements have been identified to drive expression within well-defined cell-subpopulations within the glomerulus and especially also within the different individual segments of the tubule.…”