Evaluation of the mucoadhesive properties of chitosan nanoparticles prepared using different chitosan to tripolyphosphate (CS:TPP) ratios

Hejjaji, Ezzeddin M.A.; Smith, Alan M.; Morris, Gordon A.

doi:10.1016/j.ijbiomac.2018.09.185

Cited by 96 publications

(40 citation statements)

References 56 publications

(79 reference statements)

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“…Mucin alone has a negative ζ-potential: −18.7 ± 0.6 mV [187], which is very close to the value of −19.4 mV (at pH 7) that was reported from Sogias et al [54]. CS has a positive ζ-potential close to +46.7 ± 0.4 mV, which decreases progressively by increasing the amount of TPP used for NPs preparation (vide supra) [188]. As the number of available -NH 3 + groups-which are able to ionically interact with the sialic acids of mucin-decrease, a reduced mucoadhesion is expected.…”

Section: Nanoparticles From Modified Chitosansupporting

confidence: 83%

Chitosan and its Derivatives for Ocular Delivery Formulations: Recent Advances and Developments

et al. 2020

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Chitosan (CS) is a hemi-synthetic cationic linear polysaccharide produced by the deacetylation of chitin. CS is non-toxic, highly biocompatible, and biodegradable, and it has a low immunogenicity. Additionally, CS has inherent antibacterial properties and a mucoadhesive character and can disrupt epithelial tight junctions, thus acting as a permeability enhancer. As such, CS and its derivatives are well-suited for the challenging field of ocular drug delivery. In the present review article, we will discuss the properties of CS that contribute to its successful application in ocular delivery before reviewing the latest advances in the use of CS for the development of novel ophthalmic delivery systems. Colloidal nanocarriers (nanoparticles, micelles, liposomes) will be presented, followed by CS gels and lenses and ocular inserts. Finally, instances of CS coatings, aiming at conferring mucoadhesiveness to other matrixes, will be presented.

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Section: Nanoparticles From Modified Chitosansupporting

confidence: 83%

Chitosan and its Derivatives for Ocular Delivery Formulations: Recent Advances and Developments

et al. 2020

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“…The optimal vectors not only protect the MB from degradation but also mediate target recognition of nucleic acid sequences in the cells, thereby facilitating the visualization study of live cells. CS has low toxicity and immunogenicity and can be obtained in a large quantity from the natural environment (Hejjaji et al 2019). Due to its polycationic characteristics, chitosan can form complexes with negatively charged nucleic acids through electrostatic interactions, leading to the condensation and protection of nucleic acids.…”

Section: Discussionmentioning

confidence: 99%

Detection of miR-155-5p and imaging lung cancer for early diagnosis: in vitro and in vivo study

Zhu

Fang

Guo³

et al. 2020

J Cancer Res Clin Oncol

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Purpose Currently, the routine screening program has insufficient capacity for the early diagnosis of lung cancer. Therefore, a type of chitosan-molecular beacon (CS-MB) probe was developed to recognize the miR-155-5p and image the lung cancer cells for the early diagnosis. Methods Based on the molecular beacon (MB) technology and nanotechnology, the CS-MB probe was synthesized selfassembly. There are four types of cells-three kinds of animal models and one type of histopathological sections of human lung cancer were utilized as models, including A549, SPC-A1, H446 lung cancer cells, tumor-initiating cells (TICs), subcutaneous and lung xenografts mice, and lox-stop-lox(LSL) K-ras G12D transgenic mice. The transgenic mice dynamically displayed the process from normal lung tissues to atypical hyperplasia, adenoma, carcinoma in situ, and adenocarcinoma. The different miR-155-5p expression levels in these cells and models were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The CS-MB probe was used to recognize the miR-155-5p and image the lung cancer cells by confocal microscopy in vitro and by living imaging system in vivo. Results The CS-MB probe could be used to recognize the miR-155-5p and image the lung cancer cells significantly in these cells and models. The fluorescence intensity trends detected by the CS-MB probe were similar to the expression levels trends of miR-155 tested by qRT-PCR. Moreover, the fluorescence intensity showed an increasing trend with the tumor progression in the transgenic mice model, and the occurrence and development of lung cancer were dynamically monitored by the differen fluorescence intensity. In addition, the miR-155-5p in human lung cancer tissues could be detected by the miR-155-5p MB. Conclusion Both in vivo and in vitro experiments demonstrated that the CS-MB probe could be utilized to recognize the miR-155-5p and image the lung cancer cells. It provided a novel experimental and theoretical basis for the early diagnosis of the disease. Also, the histopathological sections of human lung cancer research laid the foundation for subsequent preclinical studies. In addition, different MBs could be designed to detect other miRNAs for the early diagnosis of other tumors.

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“…It relies on electrostatic interactions between the positive charges on chitosan and the negative charges on a non-toxic cross-linker, such as sodium tripolyphosphate (TPP) [ 26 ]. When using this technique, the determination of the ratio Cs/TPP and pH of the solution is key for optimizing NPs’ physicochemical properties, such as size and zeta potential (ZP) [ 27 ]. Indeed, the size and ZP play a key role in the endocytosis of NPs by brain endothelial cells since it was found that, to be effective in crossing the BBB, NPs should have a size smaller than 200 nm and a positive ZP value [ 28 , 29 ].…”

Section: Chitosan Nanocarriersmentioning

confidence: 99%

Overcoming the Blood-Brain Barrier: Functionalised Chitosan Nanocarriers

et al. 2020

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The major impediment to the delivery of therapeutics to the brain is the presence of the blood-brain barrier (BBB). The BBB allows for the entrance of essential nutrients while excluding harmful substances, including most therapeutic agents; hence, brain disorders, especially tumours, are very difficult to treat. Chitosan is a well-researched polymer that offers advantageous biological and chemical properties, such as mucoadhesion and the ease of functionalisation. Chitosan-based nanocarriers (CsNCs) establish ionic interactions with the endothelial cells, facilitating the crossing of drugs through the BBB by adsorptive mediated transcytosis. This process is further enhanced by modifications of the structure of chitosan, owing to the presence of reactive amino and hydroxyl groups. Finally, by permanently binding ligands or molecules, such as antibodies or lipids, CsNCs have showed a boosted passage through the BBB, in both in vivo and in vitro studies which will be discussed in this review.

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Evaluation of the mucoadhesive properties of chitosan nanoparticles prepared using different chitosan to tripolyphosphate (CS:TPP) ratios

Cited by 96 publications

References 56 publications

Chitosan and its Derivatives for Ocular Delivery Formulations: Recent Advances and Developments

Chitosan and its Derivatives for Ocular Delivery Formulations: Recent Advances and Developments

Detection of miR-155-5p and imaging lung cancer for early diagnosis: in vitro and in vivo study

Overcoming the Blood-Brain Barrier: Functionalised Chitosan Nanocarriers

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