Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma

Chang, Hsueh‐Wei; Yen, Ching‐Yu; Chen, Chung‑Ho; Tsai, Jun‐Hsu; Tang, Jen‐Yang; Kao, Yu Hsun; Yy, Wang; Yuan, Shyng‐Shiou F.; Lee, Sheng‐Yang

doi:10.3892/ol.2018.9168

Cited by 27 publications

(26 citation statements)

References 50 publications

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“…Total RNA was extracted by Trizol reagent (Invitrogen, Carlsbad, California) and reverse‐transcribed by OmniScript RT kit (Qiagen, Valencia, California) as described 27 . cDNA was reacted with iQ SYBR Green Supermix (Bio‐Rad Laboratories, Hercules, California) for preforming Real‐Time RT‐PCR using a MyiQ real‐time machine (Bio‐Rad).…”

Section: Methodsmentioning

confidence: 99%

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Peng

Hsiao

Lan

et al. 2020

Environmental Toxicology

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Discovering drug candidates for the modulation of metastasis is of great importance in inhibiting oral cancer malignancy. Although most pomegranate extract applications aim at the antiproliferation of cancer cells, its antimetastatic effects remain unclear, especially for oral cancer cells. The aim of this study is to evaluate the change of two main metastasis characters, migration and invasion of oral cancer cells. Further, we want to explore the molecular mechanisms of action of pomegranate extract (POMx) at low cytotoxic concentration. We found that POMx ranged from 0 to 50 μg/mL showing low cytotoxicity to oral cancer cells. In the case of oral cancer HSC-3 and Ca9-22 cells, POMx inhibits wound healing migration, transwell migration, and matrix gel invasion.Mechanistically, POMx downregulates matrix metalloproteinase (MMP)-2 and MMP-9 Sheng-Yao Peng and Chien-Chou Hsiao contributed equally to this work.

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Section: Methodsmentioning

confidence: 99%

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Peng

Hsiao

Lan

et al. 2020

Environmental Toxicology

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“…Total RNA, prepared by Trizol reagent (Invitrogen, Carlsbad, CA, USA), was reverse-transcribed to cDNA using the OmniScript RT kit (Qiagen, Valencia, CA, USA) as described previously [25]. qRT-PCR was performed by iQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) using a MyiQ real-time machine (Bio-Rad).…”

Section: Quantitative Rt-pcr (Qrt-pcr) For Antioxidant-associated Genesmentioning

confidence: 99%

Low Concentration of Withaferin a Inhibits Oxidative Stress-Mediated Migration and Invasion in Oral Cancer Cells

Tang

Ou‐Yang

et al. 2020

Biomolecules

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Withaferin A (WFA) has been reported to inhibit cancer cell proliferation based on high cytotoxic concentrations. However, the low cytotoxic effect of WFA in regulating cancer cell migration is rarely investigated. The purpose of this study is to investigate the changes in migration and mechanisms of oral cancer Ca9-22 cells after low concentrations of WFA treatment. WFA under 0.5 μM at 24 h treatment shows no cytotoxicity to oral cancer Ca9-22 cells (~95% viability). Under this condition, WFA triggers reactive oxygen species (ROS) production and inhibits 2D (wound healing) and 3D cell migration (transwell) and Matrigel invasion. Mechanically, WFA inhibits matrix metalloproteinase (MMP)-2 and MMP-9 activities but induces mRNA expression for a group of antioxidant genes, such as nuclear factor, erythroid 2-like 2 (NFE2L2), heme oxygenase 1 (HMOX1), glutathione-disulfide reductase (GSR), and NAD(P)H quinone dehydrogenase 1 (NQO1)) in Ca9-22 cells. Moreover, WFA induces mild phosphorylation of the mitogen-activated protein kinase (MAPK) family, including extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38 expression. All WFA-induced changes were suppressed by the presence of ROS scavenger N-acetylcysteine (NAC). Therefore, these results suggest that low concentration of WFA retains potent ROS-mediated anti-migration and -invasion abilities for oral cancer cells.

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“…ITGA5, a member of the integrin alpha chain family, is known to participate in cell-surface mediated signaling. ITGA5 overexpression has been reported to be in a series of tumors (Chang et al, 2018;Yoo et al, 2016;Zhao et al, 2015). Increased expression of ITGA5 is also an independent prognostic marker for patients with GC (Ren et al, 2014).…”

Section: Survival Analysis Of Nine Hub Genesmentioning

confidence: 96%

Peer Review #3 of "In silico analyses for potential key genes associated with gastric cancer (v0.1)"

2018

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Background: Understanding hub genes involved in gastric cancer (GC) metastasis could lead to effective approaches to diagnose and treat cancer. In this study, we aim to identify the hub genes and investigate the underlying molecular mechanisms of GC. Methods: To explore potential therapeutic targets for GC, three expression profiles (GSE54129, GSE 33651, GSE81948) of the genes were extracted from the Gene Expression Omnibus (GEO) database. The GEO2R online tool was applied to screen out differentially expressed genes (DEGs) between GC and normal gastric samples. Database for Annotation, Visualization and Integrated Discovery was applied to perform Gene Ontology (GO) and KEGG pathway enrichment analysis. The protein-protein interaction (PPI) network of these DEGs was constructed using a STRING online software. The hub genes were identified by the CytoHubba plugin of Cytoscape software. Then, the prognostic value of these identified genes was verified by gastric cancer database derived from Kaplan-Meier plotter platform. Results: A total of 85 overlapped upregulated genes and 44 downregulated genes were identified. The majority of the DEGs were enriched in extracellular matrix organization, endodermal cell differentiation, and endoderm formation. Moreover, five KEGG pathways were significantly enriched, including ECMreceptor interaction, amoebiasis, AGE-RAGE signaling pathway in diabetic complications, focal adhesion, protein digestion and absorption. By combining the results of PPI network and CytoHubba, a total of nine hub genes including COL1A1, THBS1, MMP2, CXCL8, FN1, TIMP1, SPARC, COL4A1, and ITGA5 were selected. The Kaplan-Meier plotter database confirmed that overexpression levels of these genes were associated with reduced overall survival, except for THBS1 and CXCL8. Conclusions: Our study suggests that COL1A1, MMP2, FN1, TIMP1, SPARC, COL4A1, and ITGA5 may be potential biomarkers and therapeutic targets for GC. Further study is needed to assess the effect of THBS1 and CXCL8 on GC.

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Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma

Cited by 27 publications

References 50 publications

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Low Concentration of Withaferin a Inhibits Oxidative Stress-Mediated Migration and Invasion in Oral Cancer Cells

Peer Review #3 of "In silico analyses for potential key genes associated with gastric cancer (v0.1)"

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