Evaluation of the morphokinetic parameters and development of pre‐implantation embryos obtained by testicular, epididymal and ejaculate spermatozoa using time‐lapse imaging system

Buran, A; Tulay, Pınar; Dayıoğlu, Nurten; Bakırcıoğlu, Emre; Bahçeci, Mustafa; İrez, Tülay

doi:10.1111/and.13217

Cited by 7 publications

(8 citation statements)

References 50 publications

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“…Our observation on faster pronuclear appearance is in line with earlier observations [ 36 , 37 ]. Sperm DNA damage needs to be repaired in the oocyte after fertilization [ 22 , 23 ].…”

Section: Discussionsupporting

confidence: 94%

Time-lapse imaging of human embryos fertilized with testicular sperm reveals an impact on the first embryonic cell cycle

Marion

Speksnijder

Hoek

et al. 2021

Biology of Reproduction

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Testicular sperm is increasingly used during in vitro fertilization treatment. Testicular sperm has the ability to fertilize the oocyte after intracytoplasmic sperm injection (ICSI), but they have not undergone maturation during epididymal transport. Testicular sperm differs from ejaculated sperm in terms of chromatin maturity, incidence of DNA damage and RNA content. It is not fully understood what the biological impact is of using testicular sperm, on fertilization, preimplantation embryo development and post-implantation development. Our goal was to investigate differences in human preimplantation embryo development after ICSI using testicular sperm (TESE-ICSI) and ejaculated sperm. We used time-lapse embryo culture to study these possible differences. Embryos (n = 639) originating from 208 couples undergoing TESE-ICSI treatment were studied and compared to embryos (n = 866) originating from 243 couples undergoing ICSI treatment with ejaculated sperm. Using statistical analysis with linear mixed models, we observed that pronuclei appeared 0.55 hours earlier in TESE-ICSI embryos, after which the pronuclear stage lasted 0.55 hours longer. Also, significantly more TESE-ICSI embryos showed direct unequal cleavage from the 1-cell stage to the 3-cell stage. TESE-ICSI embryos proceeded faster through the cleavage divisions to the 5- and the 6-cell stage, but this effect disappeared when we adjusted our model for maternal factors. In conclusion, sperm origin affects embryo development during the first embryonic cell cycle, but not developmental kinetics to the 8-cell stage. Our results provide insight into the biological differences between testicular and ejaculated sperm and their impact during human fertilization.

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“…Our observation on faster pronuclear appearance is in line with earlier observations [ 36 , 37 ]. Sperm DNA damage needs to be repaired in the oocyte after fertilization [ 22 , 23 ].…”

Section: Discussionsupporting

confidence: 94%

Time-lapse imaging of human embryos fertilized with testicular sperm reveals an impact on the first embryonic cell cycle

Marion

Speksnijder

Hoek

et al. 2021

Biology of Reproduction

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show abstract

“…5% from D0 to D3, and then 2% from D3 to D6) in the subsequent IVF cycle. Exclusion criteria were: use of (1) testis or epididymal sperm [20][21][22] (2) preimplantation genetic diagnosis, (3) donor oocytes, and (4) absence of growing embryos at D3. In total, 120 couples were included for evaluating the impact of O 2 concentration on usable blastocyst rate (primary objective, Fig.…”

Section: Methodsmentioning

confidence: 99%

Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization

Brouillet

Baron

Barry

et al. 2021

Sci Rep

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Oxygen (O2) concentration is approximately 5% in the fallopian tube and 2% in the uterus in humans. A “back to nature” approach could increase in vitro fertilization (IVF) outcomes. This hypothesis was tested in this monocentric observational retrospective study that included 120 couples who underwent two IVF cycles between 2014 and 2019. Embryos were cultured at 5% from day 0 (D0) to D5/6 (monophasic O2 concentration strategy) in the first IVF cycle, and at 5% O2 from D0 to D3 and 2% O2 from D3 to D5/6 (biphasic O2 concentration strategy) in the second IVF cycle. The total and usable blastocyst rates (44.4% vs. 54.8%, p = 0.049 and 21.8% vs. 32.8%, p = 0.002, respectively) and the cumulative live birth rate (17.9% vs. 44.1%, p = 0.027) were significantly higher with the biphasic (5%-2%) O2 concentration strategy. Whole transcriptome analysis of blastocysts donated for research identified 707 RNAs that were differentially expressed in function of the O2 strategy (fold-change > 2, p value < 0.05). These genes are mainly involved in embryo development, DNA repair, embryonic stem cell pluripotency, and implantation potential. The biphasic (5–2%) O2 concentration strategy for preimplantation embryo culture could increase the “take home baby rate”, thus improving IVF cost-effectiveness and infertility management.

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“…Some studies reported that embryo quality of OA epididymal sperm degraded because of obstruction. Ali Buran’s and Shih’s studies showed that the epididymal sperm had poor embryo quality compared to testicular sperm in OA patients [ 9 , 35 ]. And our study also showed the developmental potential of the OEE group decreased.…”

Section: Discussionmentioning

confidence: 99%

The developmental miR-17–92 cluster and the Sfmbt2 miRNA cluster cannot rescue the abnormal embryonic development generated using obstructive epididymal environment-producing sperm in C57BL/6 J mice

Liu

et al. 2022

Reprod Biol Endocrinol

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Background Sperm, during epididymal transit, acquires microRNAs(miRNAs), which are crucial for embryonic development. However, whether sperm miRNAs influenced by an obstructive epididymal environment affect embryonic development remains unknown. Method The sham operation and vasectomy were performed in C57BL/6 J mice to create the control group (CON) and the obstructive epididymal environment group(OEE) group, respectively. The morphology of the testis and epididymis was observed using hematoxylin and eosin staining (HE staining) to establish the OEE mice model. The sperm quality test, intracytoplasmic sperm injection (ICSI), and epididymosomes fusion were employed to observe the effect of the obstructive epididymal environment on sperm and resultant embryonic development. The alteration of the sperm small RNA (sRNA) profile was analyzed by sRNA sequencing. RT-qPCR and DNA methylation were applied to observe the effect of obstructive epididymis on the expression of sperm miRNAs. The miRNAs microinjection was used to explore the impacts of sperm miRNAs on embryonic development. Results We confirmed postoperative 8-week mice as the OEE mice model by examining the morphology of the testis and epididymis. In the OEE group, we observed that sperm quality degraded and the development potential of embryos was reduced, which can be saved by the normal epididymal environment. The sperm sRNA sequencing revealed that the expression of the developmental miR-17–92 cluster and the Sfmbt2 miRNA cluster was downregulated in the OEE group. The expression of these two miRNA clusters in epididymis was also downregulated and regulated by DNA methylation. However, the downregulation of either the miR-17–92 cluster or the Sfmbt2 miRNA cluster in normal zygotes did not impair embryonic development. Conclusion The obstructive epididymal environment influences sperm quality and resultant embryonic development, as well as the abundance of the developmental miR-17–92 cluster and the Sfmbt2 miRNA cluster in sperm, but these miRNA clusters are not the cause of abnormal embryonic development. It implies that epididymis is important in early embryonic development and may play a potential role in sperm epigenome.

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Evaluation of the morphokinetic parameters and development of pre‐implantation embryos obtained by testicular, epididymal and ejaculate spermatozoa using time‐lapse imaging system

Cited by 7 publications

References 50 publications

Time-lapse imaging of human embryos fertilized with testicular sperm reveals an impact on the first embryonic cell cycle

Time-lapse imaging of human embryos fertilized with testicular sperm reveals an impact on the first embryonic cell cycle

Biphasic (5–2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization

The developmental miR-17–92 cluster and the Sfmbt2 miRNA cluster cannot rescue the abnormal embryonic development generated using obstructive epididymal environment-producing sperm in C57BL/6 J mice

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