Evaluation of the Microzone System for Simultaneous Electrophoresis of 16 Sera

Abstract

We investigated the feasibility of using the "Microzone" electrophoresis system to produce 16 instead of eight electrophorograms on a single cellulose—acetate membrane by placing eight specimens at the left of center, rotating the cellulose—acetate membrane and supporting bridge 180° horizontally, and again applying eight samples to the left of center. The membrane, now with eight samples each on anodic and cathodic sides, is electrophoresed according to the usual recommended procedure. There was a statistically demonstrated association between sample application position and results (for 96 samples). This technique is nevertheless useful for determining albumin/globulin ratios and for screening large numbers of samples. Sera with borderline or abnormal results should be rerun by the usual technique.