Evaluation of the MicroScan Rapid Yeast Identification panel

Abstract

The MicroScan Rapid Yeast Identification (RYI) panel is a 4-h microdilution system for identification of clinical yeastlike isolates. Its accuracy was evaluated by using 357 isolates encompassing 11 genera and 30 species. The RYI panel identifications were compared with those obtained by the API 20C system assisted with morphological characterization on cornmeal-Tween 80 agar. The panels were read both visually and with the AutoScan-4, a computer-controlled microplate reader. Both the RYI panel and the API 20C system correctly identified 78% of the strains within 4 and 72 h, respectively, with no additional tests. Supplementary tests recommended by the manufacturers made it possible to identify up to 96.6% (AutoScan-4) and 98.9% (API 20C) of the strains. The accuracy of the RYI panel was 99.5% with common strains and 92.1% with less common strains. The RYI panel misidentified 10 or 12 strains and failed to identify 2 or 3 strains, depending on whether it was read with the AutoScan-4 or visually. Errors occurred with one strain of Torulopsis glabrata and the less common yeasts T. candida, Candida lusitaniae, C. lambica, C. rugosa, C. stellatoidea, Cryptococcus albidus, C. laurentii, and C. uniguttulatus. Overall, the RYI panel appears to be a reliable system for identification of the more common clinical yeast isolates.

Comparison of RapID Yeast Plus System with API 20C System for Identification of Common, New, and Emerging Yeast Pathogens

“…This emphasizes the importance of using the incubation conditions and media specified by the manufacturer. In conclusion, although rapid and easy methods are needed for clinical laboratories that have not been able to switch to automated procedures for rapid yeast identification, such as the Vitek and MicroScan systems (6,9), the RapID Yeast Plus System should be used with caution when identifying the less common yeasts and yeast-like pathogens. However, some of the incorrectly identified isolates belong to the species less frequently isolated from clinical specimens.…”

Evaluation of the MicroScan enzyme‐based system for the identification of foodborne yeasts

“…The panel gave 85% correlation with the API 20C kit for all yeasts tested in one study (Land et al 1991b) and increased up to 92% for common clinical isolates. When the MicroScan system was supplemented with a few morphological and conventional tests, its accuracy reached 96.6% (St-Germain and Beauchesne 1991). Hence, it was of interest to evaluate the system for identifying foodborne yeasts.…”

“…The API and MicroScan systems have been devised to assist in identifying clinical yeast isolates. The API Yeast Ident system has been reported to provide unreliable results with clinical isolates (Salkin et al 1987;Pfaller et al 1988;Sekhon et al 1988), whereas the Microscan system appears more promising (Land et al 1991b;St-Germain and Beauchesne 1991). Evaluation of the API Yeast Ident system for identifying foodborne yeasts has revealed its limited value (Deak and Beuchat 1988;Torok and King 1991).…”

Identification of Candida species in the clinical laboratory: a review of conventional, commercial, and molecular techniques

“…Abbot Quantum II. The Quantum II system consists of a multipunch cartridge perforator, a dual-wavelength photometer that measures colorimetric changes in the individual cartridge chambers, and a single-use disposable plastic cartridge with 20 chambers containing lyophilized biochemical media (Kiehn et al, 1985;Pfaller et al, 1988) for the identification of aerobic gram-negative bacilli or yeasts (St Germain and Beauchesne, 1991). The identification of yeasts is carried out by turbidimetric or colorimetric readings of biochemical chambers after 24 h of the cartridges' inoculation (Pfaller et al, 1988).…”