Evaluation of the microbiological quality of reclaimed water produced from a lagooning system

Fernández-Cassi, Xavier; Silvera, Carolina; Cervero-Aragó, Sílvia; Rusiñol, Marta; Latif-Eugeni, F.; Bruguera-Casamada, Carmina; Civit, Sergi; Araujo, Rosa; Figueras, María José; Gironés, Rosina; Bofill-Mas, Sı́lvia

doi:10.1007/s11356-016-6812-0

Cited by 30 publications

(19 citation statements)

References 89 publications

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“…The amount of arcobacters showed a decrease of at least 2 logarithms from the influent to the effluent of the WWTP and both enumeration methods showed similar results (Figure 2). The densities of Arcobacter found in the effluent water in our study are similar to the results shown in another study performed from the same WWTP [21, 24]. No seasonal variation was observed among results (Figure 2).…”

Section: Resultssupporting

confidence: 90%

The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity ofArcobacterspp. in a Wastewater Treatment Plant

Levicán

Collado

Figueras

2016

BioMed Research International

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The genus Arcobacter includes species considered emerging food and waterborne pathogens. Despite Arcobacter has been linked to the presence of faecal pollution, few studies have investigated its prevalence in wastewater, and the only isolated species were Arcobacter butzleri and Arcobacter cryaerophilus. This study aimed to establish the prevalence of Arcobacter spp. at a WWTP using in parallel two culturing methods (direct plating and culturing after enrichment) and a direct detection by m-PCR. In addition, the genetic diversity of the isolates was established using the ERIC-PCR genotyping method. Most of the wastewater samples (96.7%) were positive for Arcobacter and a high genetic diversity was observed among the 651 investigated isolates that belonged to 424 different ERIC genotypes. However, only few strains persisted at different dates or sampling points. The use of direct plating in parallel with culturing after enrichment allowed recovering the species A. butzleri, A. cryaerophilus, Arcobacter thereius, Arcobacter defluvii, Arcobacter skirrowii, Arcobacter ellisii, Arcobacter cloacae, and Arcobacter nitrofigilis, most of them isolated for the first time from wastewater. The predominant species was A. butzleri, however, by direct plating predominated A. cryaerophilus. Therefore, the overall predominance of A. butzleri was a bias associated with the use of enrichment.

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Section: Resultssupporting

confidence: 90%

The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity ofArcobacterspp. in a Wastewater Treatment Plant

Levicán

Collado

Figueras

2016

BioMed Research International

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“…These data demonstrated that the virus was able to stay stable in waters and resistant to disinfectants. These results are consistent with the previously published studies of La ; Hamza et al, (2009);Fernandez-Cassi et al, (2016) which reported there is no significant reduction in viral concentration before and after treatment processes.…”

Section: Discussionsupporting

confidence: 94%

Prevalence of human polyomavirus and papillomavirus in wastewater and in stool of Egyptian patients

Ahmed¹,

Elmahdy²,

Allayh³

et al. 2019

Egypt. J. of Aquatic Biolo. and Fish.

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Recently oncogenic viruses that are greatly associated with human cancers were detected in urban sewage and other water environments worldwide. The direct contact with contaminated water and sewage can result in serious infections associated with a wide range of diseases. Human Papillomaviruses (HPVs) and Human Polyomaviruses (HPyVs) were the most common virusesthat have been detected in urban sewage worldwide. In the present study, Ninety sewage samples were collected from Zenin wastewater treatment plant in Giza Governorate. Sixty stool samples were obtained from cancer patients. The findings showed that the prevalence of HPVs in sewage and stool samples was 24.4 % (22/90) and 28.3 % (17/60), respectively. HPV16 genotype was the most predominant genotype using sequencing. On the other hand, the prevalence of HPyV in sewage and stool samples was 78.9% (71/90) and 58.3% (35/60) respectively. JC HPyV and BK HPyV were the most common genotypes of human polyomaviruses in sewage 57.8% (41/71), 42.3% (30/71), respectively and in stool 54.3% (19/35), 45.7% (16/35), respectively. By using quantitative Real-time PCR, the number of HPyV DNA copies ranged from 5.3x10 4 to 6.02x10 4 GC\L in raw wastewater and 6.2x10 3 to 6.85x10 3 GC\L in treated effluent of wastewater treatment plant. Regarding to stool samples, their numbers range were 2.5x10 5 and 1.24x10 7 GC\L. These results are indicator of risk for the prevalence of HPVs and HPyVs in Egyptian environment. This is the first report in Egypt to study the human oncogenic viruses in the same study of environmental and clinical samples.

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“…Conventional treatments to reclaim water at wastewater treatment plants (WWTPs), such as a primary and secondary or biological treatment, do not greatly reduce the concentration of Aeromonas [6]. However, if additional tertiary treatments are carried out such as chemical (ozone, chlorination) and physical (ultraviolet radiation) treatments, Aeromonas can be completely eliminated [82,[112][113][114]. Another natural tertiary treatment is lagooning.…”

Section: Aeromonas In Aquatic Environmentsmentioning

confidence: 99%

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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The genus Aeromonas belongs to the Aeromonadaceae family and comprises a group of Gram-negative bacteria widely distributed in aquatic environments, with some species able to cause disease in humans, fish, and other aquatic animals. However, bacteria of this genus are isolated from many other habitats, environments, and food products. The taxonomy of this genus is complex when phenotypic identification methods are used because such methods might not correctly identify all the species. On the other hand, molecular methods have proven very reliable, such as using the sequences of concatenated housekeeping genes like gyrB and rpoD or comparing the genomes with the type strains using a genomic index, such as the average nucleotide identity (ANI) or in silico DNA–DNA hybridization (isDDH). So far, 36 species have been described in the genus Aeromonas of which at least 19 are considered emerging pathogens to humans, causing a broad spectrum of infections. Having said that, when classifying 1852 strains that have been reported in various recent clinical cases, 95.4% were identified as only four species: Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). Since aeromonads were first associated with human disease, gastroenteritis, bacteremia, and wound infections have dominated. The literature shows that the pathogenic potential of Aeromonas is considered multifactorial and the presence of several virulence factors allows these bacteria to adhere, invade, and destroy the host cells, overcoming the immune host response. Based on current information about the ecology, epidemiology, and pathogenicity of the genus Aeromonas, we should assume that the infections these bacteria produce will remain a great health problem in the future. The ubiquitous distribution of these bacteria and the increasing elderly population, to whom these bacteria are an opportunistic pathogen, will facilitate this problem. In addition, using data from outbreak studies, it has been recognized that in cases of diarrhea, the infective dose of Aeromonas is relatively low. These poorly known bacteria should therefore be considered similarly as enteropathogens like Salmonella and Campylobacter.

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Evaluation of the microbiological quality of reclaimed water produced from a lagooning system

Cited by 30 publications

References 89 publications

The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity ofArcobacterspp. in a Wastewater Treatment Plant

The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity ofArcobacterspp. in a Wastewater Treatment Plant

Prevalence of human polyomavirus and papillomavirus in wastewater and in stool of Egyptian patients

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

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