Evaluation of the Microalgae Paste Viability Produced in a Mollusk Hatchery in Southern Brazil

Nunes, Moira; Pereira, Adriana; Ferreira, Jaime Fernando; Yasumaru, Fanny

doi:10.1111/j.1749-7345.2008.00226.x

Cited by 15 publications

(16 citation statements)

References 32 publications

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“…2). Este comportamiento se debería a que las células sufren menor daño físico al ser centrifugadas, resultado que se respalda en las investigaciones de Beneman et al (1980), Montaini et al (1995), Albentosa et al (1997), D`Souza et al (2000, , Molina-Grima et al (2003) y Nunes et al (2009), quienes coinciden en las ventajas comparativas de este método de cosecha.…”

Section: Discussionunclassified

“…Las pastas algales (obtenidas por centrifugación y floculación) fueron divididas en dos partes cada una y a una de las partes se le agregó 0.1% m/m de vitamina C (ácido ascórbico) con la finalidad de evaluar su utilidad como antioxidante (Nunes et al 2009). Posteriormente, todas las pastas fueron almacenadas durante cuatro semanas a -20ºC en oscuridad.…”

Section: Producción De Pastas Algales De Rhodomonas Salinaunclassified

“…(TISO) y Pavlova lutheri, se deteriora con mayor facilidad (Molina-Grima et al 1994, McCausland et al 1999. Entre los métodos utilizados para preservar la calidad nutricional de las pastas microalgales están: la aplicación de antioxidantes (Molina-Grima et al 1994, Tzovenis et al 2004, control de luz (Montaini et al 1995, Chini Zittelli et al 2003, liofilización (Albentosa et al 1997), temperatura (McCausland et al 1999, aireación (Ponis et al 2008), congelación (Lubzens et al 1995) y refrigeración (Robert et al 2001, Nunes et al 2009.…”

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Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)

Guevara¹,

Bastardo²,

Cortez³

et al. 2010

RBT

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Rhodomonas salina (Cryptophyta) pastes as feed for Brachionus plicatilis (Rotifera). Rotifers are an important live feed for first feeding larvae of many fish species. The use of concentrated algae cells in the mass culture of the rotifer Brachionus plicatilis (Brachionidae) has opened new horizons for research on this organism. Pastes of Rhodomonas salina (Pyrenomonadaceae) obtained either by centrifugation or flocculation with chitosan were preserved, with or without vitamin C, at-20°C for four weeks and were evaluated biochemically (proteins, lipids, pigments and fatty acids contents) and subsequently, were used to feed the rotifer Brachionus plicatilis at a ratio of 25mg/L/day. Four different microalgae pastes were prepared: (1) centrifuged and preserved with vitamin C (CV), (2) centrifuged and preserved without vitamin C (C), (3) flocculated and with vitamin C (FV) and (4) flocculated without vitamin C (F). All treatments showed similar contents of proteins and total lipids with respect to control culture (a fresh culture of R. salina), with mean values of 40.0±2.32% and 12.0±1.45%, respectively. The pheophytin a/chlorophyll a ratio, a general indicator of the chemical status of microalgal concentrates, was similar (0.09-0.11) between centrifuged pastes and control culture, but was found to be higher in flocculated pastes (1.28-1.48). The fatty acid profile varied with respect to the control culture, mainly in the proportion of the essential polyunsaturated fatty acids (PUFAs): eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Total PUFAs, EPA and DHA contents were statistically similar between centrifuged pastes and control culture (PUFAs: 47%, EPA: 4% and DHA: 4.7%), whereas values obtained for flocculated pastes were significantly lower. The rotifers grew equally well when fed with centrifuged pastes or control culture (maximum density: 320rotifers/mL; instantaneous growth rate: 0.23rotifers/ day, fecundity: 1.49eggs/female and productivity: 43x10 3 rotifers/L/day. No significant effect of vitamin C was found when used as a paste preservative. We concluded that centrifugation is an effective harvesting method, and that freezing to-20ºC for four weeks (no vitamin added), may help maintain the nutritional quality of R. salina paste, similar to fresh microalgae and can be offered to Brachionus plicatilis.

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Section: Discussionunclassified

Section: Producción De Pastas Algales De Rhodomonas Salinaunclassified

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Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)

Guevara¹,

Bastardo²,

Cortez³

et al. 2010

RBT

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“…Once the flocculated concentrate is obtained, suitable preservation is required to maintain nutritional value for a reasonable time. Previously, several methods have been used to preserve microalgal concentrates from on-site production, such as adding additives or preservatives (Tzovenis et al 2004), freezing (Heasman et al 2000) and refrigeration (Nunes et al 2009). In this study, the simple technique of storage in a refrigerator at 4°C was selected as this method is easy and cost-effective for use in a hatchery.…”

Section: Flocculated Concentrates Preservationmentioning

confidence: 99%

Evaluation of flocculated concentrates from intensive shrimp pond water as a substitute for microalgal concentrates in the nursery culture of juvenile oyster (Crassostrea belcheri)

2016

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Intensive shrimp pond water typically contains high densities of microalgae and may have a great potential as a substitute for microalgae in the nursery culture of juvenile oysters as this stage needs large amount of microalgae. Two experiments with different phytoplankton composition in flocculated concentrates from shrimp pond water were investigated to compare the effect on preservation and use of flocculated concentrates from intensive shrimp pond as a partial substitute for microalgae concentrates for the nursery culture of juvenile oysters (Crassostrea belcheri) was evaluated. The results show that total crude protein content in flocculated concentrates mostly contain unidentified nanocyanobacteria preserved in refrigerator at 4°C gradually decreased from the first week in experiment 1, while a slight decrease in crude protein content appeared after the third week in flocculated concentrates dominated by the Bacillariophyceae and unidentified nanocyanobacteria groups in experiment 2. The pheophytin a:chlorophyll a ratio suddenly increased during the third week of storage and most of the fatty acid composition was absent after four weeks of storage in both experiments. Substituting 75 % of microalgal concentrates in feed with flocculated concentrates from shrimp pond water showed no significant differences in growth performance in experiment 1, but higher growth performance in experiment 2 in comparison with control treatment (100 % microalgal concentrates). Our findings indicate that a partial substitution of flocculated concentrates of up to 75 % from shrimp pond water for microalgal concentrates would lead to better growth in juvenile oysters.

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“…(Bhattarai et al 2007), and evaluating diatom paste stored at 4 C without additives for the diatoms Chaetoceros muelleri, Skeletonema sp., and Chaetoceros calcitrans for use in a mollusk hatchery. In the latter study, the storage of microalgae pastes yielded a viability rate of greater than 98% after 6-7 wk compared with 2 wk of viability in pastes that were stored at 4 C in 0.1% ascorbic acid (Nunes et al 2009). Chen (2001) used a storage method to reduce the time and economic costs that are associated with immobilized cells of Scenedesmus quadricaudata.…”

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confidence: 91%

Cold Storage of Six Marine Benthic Diatoms Native to the Mexico Pacific Coast

Núñez-Zarco¹,

Sánchez‐Saavedra²

2011

J World Aquaculture Soc

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Cultures of six benthic diatoms were maintained in the dark to measure their viability and biochemical composition after 8 wk of storage at low temperature (4 C) in darkness by refrigeration. Cell density, growth rate, and viability for each benthic diatom changed significantly after storage. Significant differences were observed with regard to cell size (length and width) of Nitzschia laevis, Navicula sp., and Amphora tenerrima as a result of storage. In general, the proximal composition of the benthic diatom cultures changed after week 1 of storage and decreased after week 4 of storage for all the diatoms. These results demonstrate that under 1-4 wk of storage these diatoms maintain their viability and had changes in their proximal composition in species-specific responses. Storage of preserved live microalgae cells is an alternative technique that can be used to reduce the need for continuous maintenance of live cultures and can provide live feed stock for aquaculture applications.

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Evaluation of the Microalgae Paste Viability Produced in a Mollusk Hatchery in Southern Brazil

Cited by 15 publications

References 32 publications

Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)

Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)

Evaluation of flocculated concentrates from intensive shrimp pond water as a substitute for microalgal concentrates in the nursery culture of juvenile oyster (Crassostrea belcheri)

Cold Storage of Six Marine Benthic Diatoms Native to the Mexico Pacific Coast

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