Evaluation of the Merlin, Micronaut System for Automated Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa and Burkholderia Species Isolated from Cystic Fibrosis Patients

Häußler, Susanne; Ziesing, Stefan; G, Rademacher; Hoy, L.; Weißbrodt, H.

doi:10.1007/s10096-003-0974-7

Cited by 17 publications

(8 citation statements)

References 11 publications

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“…In this study, 86 P. aeruginosa isolates were tested for susceptibility to six anti-pseudomonal drugs using Merlin, Micronaut System [5,6]. In this series, the prevalence of multidrug-resistant isolates was 2 %.…”

Section: Resultsmentioning

confidence: 99%

“…For automatic measurement and identification, the SARAMIS™ (spectral archived microbial identification system) database application from bioMérieux SA France was applied. Minimal inhibitory concentrations (MICs) of piperacillin/tazobactam (PIP/TAZ), ceftazidime (CAZ), tobramycin (TOB), amikacin (AMK), ciprofloxacin (CIP) and meropenem (MEM) were determined by the broth microdilution method (Micronaut Merlin) as previously described and interpreted using the criteria recommended by the Clinical and Laboratory Standards Institute (CLSI) [5,6]. Multidrug-resistant isolates were defined as isolates intermediate or resistant to at least three drugs in the following classes: β-lactams, carbapenems, aminoglycosides and fluoroquinolones.…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

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Molecular epidemiology ofPseudomonas aeruginosa in cystic fibrosis patients from Southeast Austria

Masoud-Landgraf

Badura

Eber

et al. 2012

Wien Klin Wochenschr

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Pseudomonas aeruginosa is the major pathogen in the cystic fibrosis (CF) lung, the predominant source of its acquisition, however, is under discussion. In order to study the molecular epidemiology, we evaluated 86 P. aeruginosa isolates from 43 CF patients from southeast Austria. The DiversiLab system was used to identify genetic relationships among the isolates. Antibiotic susceptibilities were tested with a broth microdilution method (Micronaut Merlin). A total of 39 unrelated P. aeruginosa genotypes were found of which 34 were unique to a single patient and one was unique to a sibling pair. We found low rates of resistance for β-lactams with resistance to piperacillin/tazobactam and ceftazidime ranging from 4 to 6%. Resistance rates for meropenem and ciprofloxacin were 11% and 15%, respectively. The prevalence of multidrug-resistant isolates was 2%. We conclude that the majority of P. aeruginosa isolates from CF patients originate from environmental sources and patient-to-patient spread is very uncommon in our centre.

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Section: Resultsmentioning

confidence: 99%

Section: Patients Materials and Methodsmentioning

confidence: 99%

Molecular epidemiology ofPseudomonas aeruginosa in cystic fibrosis patients from Southeast Austria

Masoud-Landgraf

Badura

Eber

et al. 2012

Wien Klin Wochenschr

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“…Therefore, we evaluated the accuracy of the MICRONAUT system for direct AST of positive blood cultures under routine conditions in a clinical microbiology laboratory. The MICRONAUT system is a commercially available, automated, microtiter plate-based broth dilution AST system (2,16). Altogether, 850 positive blood cultures were investigated on a daily basis including weekends during a period of 9 months.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Merlin MICRONAUT System for Rapid Direct Susceptibility Testing of Gram-Positive Cocci and Gram-Negative Bacilli from Positive Blood Cultures

et al. 2007

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Bloodstream infections are life-threatening conditions which require the timely initiation of antimicrobial therapy. Inappropriate initial antimicrobial therapy of septic patients is associated with adverse outcomes (13,15,20). Automated blood culture systems that monitor blood culture bottles continuously for bacterial growth minimize the time necessary to detect positive blood cultures. Once bacterial growth is detected in blood cultures, rapid identification and susceptibility testing of the isolate are important tasks for the clinical microbiology laboratory. Reducing the turnaround time of microbiological analysis by using automated systems can lead to significant reductions in patient morbidity, mortality, and costs (3, 9, 27).While standard antimicrobial susceptibility testing (AST) of bacteria commonly involves pure overnight subcultures, preparation of the inoculum for automated susceptibility testing directly from the positive blood culture appears extremely attractive with respect to the time to results. Thus, direct antimicrobial susceptibility testing of isolates from positive blood cultures with many automated testing systems, like the Phoenix (BD, Heidelberg, Germany), the VITEK and VITEK 2 (BioMérieux, Nürtingen, Germany), the Sensititre (Trek Diagnostics, West Lake, OH), and the MicroScan (Dade Behring, Eschborn, Germany) systems, has been evaluated previously (4-6, 12, 14, 18, 21, 24, 26, 28). In general, good agreement between direct and standard susceptibility testing results was observed when gram-negative bacilli were tested, including both members of the Enterobacteriaceae and Pseudomonas species (4-6, 12, 14, 18, 21, 24, 26, 28). For direct testing of gram-positive cocci from blood cultures, only limited data from small studies are available for the VITEK, the VITEK 2, the Sensititre, and the MicroScan systems (5,6,8,18,26,29). A significantly higher rate of disagreement between direct and standard testing results for gram-positive cocci than for gramnegative bacilli was found. Reporting of false susceptibility of staphylococci to oxacillin and of enterococci to various antibiotics (18, 26) is a major problem with enormous clinical relevance. Since gram-positive cocci cause the majority of bloodstream infections (23, 29), rapid and reliable automated susceptibility testing of gram-positive bacteria is highly desirable.We evaluated the automated MICRONAUT system (Merlin, Bornheim-Hesel, Germany) for rapid direct microtiter broth susceptibility testing of gram-positive cocci and gram-negative bacilli from BACTEC bottles with positive blood cultures. The study was conducted under routine working conditions in the clinical microbiological laboratory of the University Hospital of Ulm, Ulm, Germany, during a 9-month period and included 850 positive blood cultures.

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“…However, up-to-date studies evaluating newer generations of automated systems in the field of CF microbiology are not available. The Merlin Micronaut system was shown to be reliable for P. aeruginosa CF isolates [39][40]. In 2005 revised CLSI guidelines have been released that for the first time include an approval of the disc diffusion for the testing of B.…”

Section: Columbia Colistin-nalidixic Acid Agar Lipase-salt-mannitol mentioning

confidence: 99%

Screening of photochemically grafted polymer films for compatibility with osteogenic precursor cells

Adden

Hoffmann

Groß

et al. 2007

Journal of Biomaterials Science, Polymer Edition

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Background: The goal of this pilot study was to design an external quality assessment (EQA) scheme for German cystic fibrosis (CF) clinical microbiology laboratories. Therefore, a multicentre-study of 18 German CF laboratories was performed to evaluate their proficiency in analysing CF respiratory secretions. Methods:Simulated clinical specimens containing a set of four frequent CF pathogens, namely two Pseudomonas aeruginosa strains differing in morphotype (mucoid versus non-mucoid) and resistotype, one Staphyloccocus aureus strain and one Burkholderia multivorans strain, were distributed to each laboratory. Isolation, identification and antimicrobial susceptibility testing (AST) of any bacterial pathogen present and completion of a questionnaire about applied microbiological protocols were requested.Results: Three of four strains were isolated and identified correctly by almost all laboratories. B. multivorans was once misidentified as B. cenocepacia. Fourteen laboratories failed to detect the second multidrug resistant P. aeruginosa isolate. AST errors occured most often for P. aeruginosa 2 followed by B. cepacia complex, P. aeruginosa 1 and S. aureus.Evaluation of the questionnaires revealed major differences in cultivation and identification techniques applied by the participating laboratories. Conclusions:A periodical EQA programme for German CF laboratories and standardized microbiological procedures seem to be necessary to advance diagnostic microbiology employed on CF respiratory tract specimens and may help to improve antiinfective treatment and infection control practices for CF patients.3

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Evaluation of the Merlin, Micronaut System for Automated Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa and Burkholderia Species Isolated from Cystic Fibrosis Patients

Cited by 17 publications

References 11 publications

Molecular epidemiology ofPseudomonas aeruginosa in cystic fibrosis patients from Southeast Austria

Molecular epidemiology ofPseudomonas aeruginosa in cystic fibrosis patients from Southeast Austria

Evaluation of the Merlin MICRONAUT System for Rapid Direct Susceptibility Testing of Gram-Positive Cocci and Gram-Negative Bacilli from Positive Blood Cultures

Screening of photochemically grafted polymer films for compatibility with osteogenic precursor cells

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