Evaluation of the local inflammatory events induced by BpirMP, a metalloproteinase from Bothrops pirajai venom

Multifunctional Toxins in Snake Venoms and Therapeutic Implications: From Pain to Hemorrhage and Necrosis

“…Acute pain through ASIC1 activation (Bohlen et al, 2011) Inflammatory pain, thermal hypersalgesia and mechanical allodynia (Zhang et al, 2017) Excitation of sensory neurons (Bohlen et al, 2011;Zhang et al, 2017) Neurogenic inflammation (Camara et al, 2003;Camargo et al, 2008) Non-neurogenic inflammation Endematogenic and pro-inflammatory (Camara et al, 2003;Casais-E-Silva et al, 2016;Costa et al, 2017) Inhibits platelet aggregation (Teixeira et al, 2011) Phospholipase activity (Gutierrez and Ownby, 2003) Myotoxic (Harris and Maltin, 1982) Pre-synaptic toxin, block of neuromuscular transmission leading to muscle paralysis (Sribar et al, 2014;Gutierrez et al, 2017) SVMP Inflammatory hyperalgesia (Fernandes et al, 2007;Bernardes et al, 2015;De Toni et al, 2015;Ferraz et al, 2015) Endematogenic activity independent of pro-inflammatory mediators (Laing et al, 2003) Cleavage of basement membrane of capillary vessels and endothelial cells adhesion proteins (Gutierrez et al, 2005;Escalante et al, 2011) Procoagulant through activation of prothrombin and Factor X (Takeda et al, 2012;Ainsworth et al, 2018) Inhibition of platelet aggregation (Kamiguti, 2005) Dermonecrotic activity dependent on TNF signaling (Laing et al, 2003) Potential paralysis through inhibition of α-7 neuronal AChR by the cysteine-rich and disintregin-like domains complex (Brust et al, 2013) SVSP Mild mechanical hyperalgesia (Menaldo et al, 2013) Leucocyte migration (Menaldo et al, 2013) Mild edema (Zychar et al, 2010) Procoagulant through activation of prothrombin and factors VII and X (Kini, 2005) Anti-coagulant through activation of Prot...…”

“…Some SVMPs also induce inflammation, including edema, and pain by triggering hyperalgesia (Dale et al, 2004;da Silva et al, 2012;Bernardes et al, 2015). The inflammation induced by jararhagin, a class P-IIIb metalloproteinase with potent hemorrhagic and dermonecrotic activity isolated from Bothrops jararaca, produced TNF-α and IL-1β in vivo (Laing et al, 2003;Clissa et al, 2006), while BJ-PI2, isolated from the same snake species, lacked hemorrhagic and necrotic activities but still induced vascular permeability and inflammatory cell migration (da .…”

“…The mechanisms on how neurogenic inflammation is triggered by the snake venom components and how it participates in the hemorrhagic process are still not understood. Pain induced by SVMPs is characterized by hyperalgesia and inflammatory pain, which is dependent on the production of cytokines, nitric oxide, prostaglandins, histamine, leukotrienes, and migration of leukocytes, mast cell degranulation and NFkB activation (Fernandes et al, 2007;Bernardes et al, 2015;De Toni et al, 2015;Ferraz et al, 2015). However, the mechanisms underlying SVMP-induced pain are still poorly understood, with neurogenic inflammation and neuronal excitatory properties still underexplored.…”

Rastreamento em células in vitro de uma fosfolipase A2 da peçonha de Bothrops alternatus por meio de pontos quânticos de tamanhos mágicos

“…A ação das svPLA 2 sobre os tecidos do organismo gera distúrbios fisiopatológicos após o envenenamento ofídico, como efeitos miotóxicos, cardiotóxicos, neurotóxicos e alterações inflamatórias e hemostáticas (MAMEDE et al 2016;BERNARDES et al, 2015;MOREIRA et al, 2014;RODRIGUES et al 2014;TONELLO et al, 2012;RIGONI et al, 2008;MONTECUCCO et al, 2008;.…”

Propriedades inflamatórias do veneno da serpente >i<Bitis arietans>/i<: contribuição dos mediadores lipídicos no envenenamento >i<in vivo>/i< e ação de toxinas isoladas do veneno em macrófagos humanos

“…Contudo, sabendo que a jararhagina é uma metaloprotease da classe P-III, portanto, mais complexa que a SMVP de 23,5 kDa isolada neste trabalho, a capacidade desta em clivar e promover a degradação das citocinas deve ser investigada. Em adição, estudos prévios também encontraram IL-10 após a administração de SVMPs isoladas de venenos botrópicos 355,356. Menaldo e cols.…”