Evaluation of the Levels of Metabolites in Feces of Patients with Inflammatory Bowel Diseases

Zhgun, E. S.; Kislun, Y V; Калачнюк, Т. Н.; Veselovsky, Vladimir A.; Urbán, A; Tikhonova, Polina; Pavlenko, Alexander V.; Ilchenko, G. N.; Ilina, Elena N.

doi:10.1134/s1990750820040113

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…The content of SCFAs in feces is becoming an important clinical indicator to judge the intestinal health status of patients with IBDs, which include UC and Crohn's disease (Zhgun et al, 2020). Low levels of SCFAs may lead to a series of problems such as reduced intestinal peristalsis, barrier dysfunction, and excessive inflammation (Parada Venegas et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Alleviative mechanism and effect of Bifidobacterium animalisA6 on dextran sodium sulfate‐induced ulcerative colitis in mice

Wuri

Fang

et al. 2022

Food Science & Nutrition

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Probiotics have been increasingly investigated for their role in alleviating symptoms of ulcerative colitis (UC), but the specific mechanism involved remains unclear. We investigated the alleviating effect of Bifidobacterium animalis A6 (BAA6) in UC through a mouse dextran sulfate sodium (DSS) model. When treated with a high dose of BAA6 (1 × 10 10 cfu/ml), it was found that colitis symptoms were significantly alleviated, and mucosal damages experienced obvious relief. Moreover, a high dose of BAA6 effectively upregulated free fatty acid receptors 2 and 3 (FFAR2 and FFAR3) expression and butyric acid metabolism specifically. Furthermore, the supplement of BAA6 significantly suppressed pro‐inflammatory cytokines levels (interleukin‐13) and the expression of pore‐forming protein claudin‐2. The upstream regulatory genes of claudin‐2, such as STAT6 , GATA4 , Cdx2 , were also significantly inhibited by BAA6. Collectively, this study concludes that BAA6 attenuated DSS‐induced colitis by increasing the levels of intestinal butyric acid, activating the butyric acid‐FFAR pathway, suppressing excessive proinflammatory response, and protecting the function of the colon epithelial barrier.

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Section: Discussionmentioning

confidence: 99%

Alleviative mechanism and effect of Bifidobacterium animalisA6 on dextran sodium sulfate‐induced ulcerative colitis in mice

Wuri

Fang

et al. 2022

Food Science & Nutrition

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Optimization of Conditions for Human Bacterial Preparation for Biological Correction of Intestinal Microflora

Sorokina¹,

Zhgun²,

Kislun³

et al. 2021

BMCRM

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Fecal microbiota transplantation (FMT) is now considered as an effective tool for the treatment of various GI pathologies. Fecal preparations are delivered both through the lower GIT (enema, colonoscopy) and upper (endoscopy, capsules). A common disadvantage of instrumental methods of administration is their high invasiveness associated with the risk of intestinal perforation and the use of anesthesia. Oral capsules are minimally invasive, comfortable and more aesthetic, so this method of drug delivery is gaining popularity. The main issue with the use of frozen feces (including the lyophilisate used in capsules) is its efficiency compared to the original material. During lyophilization, cells are exposed to stress factors such as low temperatures, water crystallization, osmotic stress, changes in pH, and dehydration. To reduce the likelihood of cell damage during lyophilization, protective media (lyo-protectants) are used. In this work sucrose, gelatin, and their combinations have been used as lyoprotectors. To estimate the number of microorganisms, a bacteriological study was carried out. The number of Bifidobacteria, Lactobacilli, and the total number of E.coli and Enterobacteriaceae was estimated. It was found that the lyophilized stool sample containing 10% sucrose as a protective medium had the highest number of viable cells. Also, the physical properties of the lyophilisate (its flowability) are convenient for preparing capsulated form. The molar ratios of short chain fatty acids (SCFAs) in the original fecal samples and lyophilisates were studied by gas chromatography. The molar ratios of major SCFAs (acetate, propionate and butyrate) were identical in the samples studied. The composition of the protective medium in which the lyophilized biomaterial corresponds to the original feces in terms of the number of "live" microorganisms has been proposed. According to its physical characteristics lyophilisate is convenient for capsules preparation.

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Evaluation of the Levels of Metabolites in Feces of Patients with Inflammatory Bowel Diseases

Cited by 2 publications

References 43 publications

Alleviative mechanism and effect of Bifidobacterium animalisA6 on dextran sodium sulfate‐induced ulcerative colitis in mice

Alleviative mechanism and effect of Bifidobacterium animalisA6 on dextran sodium sulfate‐induced ulcerative colitis in mice

Optimization of Conditions for Human Bacterial Preparation for Biological Correction of Intestinal Microflora

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