Evaluation of the interferon-gamma (IFN-γ) assay to diagnose Mycobacterium bovis infection in pigs

Pesciaroli, Michele; Russo, Marco; Mazzone, Piera; Aronica, V.; Fiasconaro, M.; Boniotti, Maria Beatrice; Corneli, Sara; Cagiola, Monica; Pacciarini, M. L.; Marco, V. Di; Pasquali, Paolo

doi:10.1016/j.vetimm.2012.06.020

Cited by 18 publications

(14 citation statements)

References 20 publications

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“…Thus, the IFN-␥ assay yielded a sensitivity of 100%. Despite that, this sensitivity was higher than the one reported for freerange domestic pigs in Italy, which was 79 to 85% (23); however, the conditions of the two studies are different, i.e., controlled laboratory versus field conditions. It is also important to note that all pigs tested negative to IFN-␥ at T2 (i.e., prior to challenge).…”

Section: Discussioncontrasting

confidence: 63%

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Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis

Beltrán‐Beck¹,

Romero²,

Boadella³

et al. 2014

Clin. Vaccine Immunol.

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f Mycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the responses of pigs with and without a history of tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain, to compare pig and wild boar responses using the same vaccination model as previously used in the Eurasian wild boar (Sus scrofa), to evaluate the use of several enzymelinked immunosorbent assays (ELISAs) and lateral flow tests for in vivo TB diagnosis in pigs, and to verify if these tests are influenced by oral vaccination with inactivated M. bovis. At necropsy, the lesion and culture scores were 20% to 43% higher in the controls than those in the vaccinated pigs. Massive M. bovis growth from thoracic tissue samples was observed in 4 out of 9 controls but in none of the 10 vaccinated pigs. No effect of the presence or absence of tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. The serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivities of the ELISAs and dual path platform (DPP) assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between the pigs with and without tonsils. In the vaccinated group, the mRNA levels for chemokine (C-C motif) receptor 7 (CCR7), interferon beta (IFN-␤), and methylmalonyl coenzyme A mutase (MUT) were higher in pigs with tonsils. Complement component 3 mRNA levels in peripheral blood mononuclear cells (PBMC) increased with vaccination and decreased after M. bovis challenge. This information is relevant for pig production in regions that are endemic for M. bovis and for TB vaccine research.

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Section: Discussioncontrasting

confidence: 63%

“…In pigs, tests based on cell-mediated immunity are commonly used in some countries (e.g., Italy [23]). However, antibody detection by ELISA and/or immunochromatographic tests is not frequent in pigs.…”

mentioning

confidence: 99%

Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis

Beltrán‐Beck¹,

Romero²,

Boadella³

et al. 2014

Clin. Vaccine Immunol.

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“…The prevalence of M. bovis infection in pigs has declined sharply in countries with effective bovine tuberculosis control in cattle, supporting the assumption that they are dead-end hosts (Cousins 2001;Pesciaroli et al 2012). Exceptions to this trend are reported from developing countries.…”

Section: Mycobacterium Bovis Infections At the Animal/ Human Interfacementioning

confidence: 84%

Zoonotic Aspects of Tuberculosis: Disease of the past or re-emerging zoonosis?

Michel

2014

Zoonoses - Infections Affecting Humans and Animals

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Tuberculosis is a serious chronic disease of humans and animals caused by members of the Mycobacterium tuberculosis complex. While M. tuberculosis affects primarily humans, Mycobacterium bovis has a wide range of host species including wild and domestic animals and humans. This chapter investigates the role of different host species in the transmission of tuberculosis at various animal/human interfaces. While drivers and modes of zoonotic TB transmission are reviewed, a broad spectrum of complexities hampering the eradication of this disease are highlighted. The most important constraints, next to factors related to pathogen-host interactions such as HIV co-infection and multidrug resistance of MTBC strains, are socio-economic shortcomings in the control of bovine tuberculosis in the cattle population as well as traditional customs and habits related to the consumption of milk and other animal products.

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“…The study, however, highlighted the difficulties related to the restraint of the animals that make this test scarcely applicable to the routine bTB testing in the field. Conversely, in a previous study (10), our group already tested IGRA performances on pigs. The study showed that IGRA is an easy-to-apply test, requiring just a blood sampling, and suggested the possible large-scale use of IGRA in pigs, due to the high diagnostic power and the reliable accuracy (10).…”

Section: Introductionmentioning

confidence: 99%

Intra-vitam Diagnosis of Tuberculosis in Pigs: Concordance Between Interferon-Gamma Release Assay and Comparative Tuberculin Skin Test

et al. 2020

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The role of pigs in the maintenance of bovine tuberculosis caused by Mycobacterium bovis has been demonstrated in many settings; however, the current control programs usually do not state any intra-vitam diagnostic procedure in this species, as for the cattle. Carcass inspection has shown to be insufficient to detect infection in swine; thus, the assessment of intradermal tuberculin test and interferon-gamma release assay (IGRA) in this species is mandatory. The current study compares the performances of the single intradermal comparative cervical tuberculin (SICCT) test and IGRA. A total of 628 Nebrodi Black pigs raised in free-roaming farms were subjected to the two tests simultaneously. Besides, 124 animals underwent postmortem examination for the detection of tuberculous lesions and isolation of mycobacteria from target organs. The two tests showed a concordance of 94.42% with a Cohen's k coefficient of 0.786 and McNemar chi-square of 4.83 (P = 0.03). Slightly lower levels of concordance (90.32%) between SICCT and IGRA were obtained in the group of 124 animals, with a Cohen's k = 0.797 and McNemar chi-squared value of 0.69 with a non-significant P = 0.41. Moreover, the results showed how IGRA tends to result positive in higher rates, mostly when non-tuberculous mycobacteria (NTM) were isolated, suggesting a possible impairment of specificity in the event of coinfections in the swine. In conclusion, the results obtained support the possibility of the strategic use of IGRA or SICCT in combination or alternatively one to the other, particularly IGRA which showed lower specificity but has evident advantages over SICCT.

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Evaluation of the interferon-gamma (IFN-γ) assay to diagnose Mycobacterium bovis infection in pigs

Cited by 18 publications

References 20 publications

Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis

Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis

Zoonotic Aspects of Tuberculosis: Disease of the past or re-emerging zoonosis?

Intra-vitam Diagnosis of Tuberculosis in Pigs: Concordance Between Interferon-Gamma Release Assay and Comparative Tuberculin Skin Test

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