Evaluation of the interactions between collagen and the surface of a bioactive glass during in vitro test

Abstract

Interactions between biomaterials and the body environment usually determine the lifetime and performance of biomedical devices used as implants. Among a variety of different types of interactions that occur whenever biomaterials are implanted into the body, adsorption of biomacromolcules onto the surface of biomaterials can guide other relevant processes such as cell attachment, cell differentiation, cell proliferation, tissue growth and biomaterials degradation. In this work, fundamental mechanisms associated with the interactions between collagen and the surface of biomaterials were studied by using Atomic Force Microscopy (AFM). The ultimate goal of this work is to isolate and simulate phenomena occurring at the molecular level, when biological entities are brought in contact with biomaterials. These results can then be used to design and test new biomedical devices. This manuscript reports the evaluation of interactions between collagen and the surface of bioactive glasses. These bioactive glasses are known to bond to soft and hard tissues through the formation of a hydroxy-carbonate-apatite layer. Collagen was grafted onto AFM cantilevers. AFM force-displacement curves were obtained using these modified tips against the surface of a bioactive glass at different stages of an in vitro test performed in a simulated body fluid. The results showed that the structure of the bioactive glass surface affects the adsorption of collagen on the biomaterial. The magnitude of the adhesion force measured is highly dependent on the deposition of a hydroxyl-carbonate-apatite at the surface of the glass.

Effectiveness of a calcium sodium phosphosilicate containing prophylaxis paste in reducing dentine hypersensitivity immediately and 4 weeks after a single application: a double‐blind randomized controlled trial

“…The initial reactivity of CSPS particles was found to form a negative charge on the surface of exposed dentine in vitro, enabling establishment of covalent bindings of CSPS to side groups of Type 1 dentinal collagen fibres (Orefice et al. ). Local precipitation of apatite‐like material was attributed to immediate release of sodium ions when CSPS comes in contact with water or saliva.…”

Bioadhesion of various proteins on random, diblock and triblock copolymer surfaces and the effect of pH conditions

“…At the same time, the polymer chains experience stress and the protein molecule may also be stretched. The stretching of the protein during pull-off could be more significant in collagen, which is a fibrous protein [36]. This may be less prominent in the globular proteins, fibronectin and BSA.…”

Soft tissue adaptation to modified titanium surfaces

“…Bioactive glass was discovered by Hench16, 17 in 1969 and found to have the ability to bind to bone18–20 as well as in some cases to soft tissues 16, 21–23. It is the ability of bioglass (BG) to react with physiologic fluids and to form tenacious bonds through cellular activity that results in application for biomedical implants 16.…”