Evaluation of the InnoTyper® 21 genotyping kit in multi-ethnic populations

Ristow, Peter Gustav; Barnes, Nicole; Murphy, Gina Pineda; Brown, Hiromi; Cloete, Kevin Wesley; D’Amato, María Eugenia

doi:10.1016/j.fsigen.2017.06.002

Cited by 7 publications

(1 citation statement)

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“…This is why many authors suggests InnoTyper® 21 as a valuable complement to autosomal STRs [ 12 , 35 , 38 ]. Some even propose that the power of discrimination offered by InnoTyper® 21 is higher than the provided by mitochondrial DNA [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the usefulness of insertion-null markers in critical skeletal remains

Haarkötter,

Saiz,

Gálvez

et al. 2024

Int J Legal Med

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Forensic DNA analysis in compromised skeletal remains may pose challenges due to DNA degradation, often resulting in partial or negative autosomal STRs profiles. To address this issue, alternative approaches such as mitochondrial DNA or SNPs typing may be employed; however, they are labour-intensive and costly. Insertion-null alleles (INNULs), short interspersed nuclear elements, have been suggested as a valuable tool for human identification in challenging samples due to their small amplicon size. A commercial kit including 20 INNULs markers along with amelogenin (InnoTyper® 21) has been developed. This study assesses its utility using degraded skeletal remains, comparing the results obtained (the number of detected alleles, RFU values, PHR, and the number of reportable markers) to those obtained using GlobalFiler™. Subsequently, the random match probability of the two profiles for each sample was determined using Familias version 3 to evaluate the power of discrimination of the results obtained from each kit. In every sample, InnoTyper® 21 yielded more alleles, higher RFU values, and a greater number of reportable loci. However, in most cases, both profiles were similarly informative. In conclusion, InnoTyper® 21 serves as a valuable complement to the analysis of challenging samples in cases where a poor or negative profile was obtained.

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