Evaluation of the induction of specific cytotoxic T lymphocytes following immunization of F1 hybrid mice with rabies antigens

Modulation of antiviral immune responses by exogenous cytokines: effects of tumour necrosis factor- , interleukin-1 , interleukin-2 and interferon- on the immunogenicity of an inactivated rabies vaccine

“…challenge (Wunderli et al, 19911). Although antigens from inactivated virus preparations are probably processed by the endocytic pathway and presented by class II molecules to CD4 + helper T cells, we could detect CD8 + CTL activity in vaccinated mice, as have other authors (Wiktor et al, 1977;Morgeaux et al, 1989;Sugamata et at., 1990;MacFarlan et al, 1986). Immunoprotection against rabies encephalomyelitis has also been suggested to result from antibody-mediated disruption of viral cell-tocell spread and inhibition of viral RNA transcription (Dietzschold et al, 1992).…”

“…However, Dietzschold and coworkers (1992) have suggested that it is not solely correlated with neutralizing activity of antibodies but also with their ability to prevent virus spread between cells and to inhibit transcription of viral RNA. Other investigators have demonstrated the induction of rabies virus-specific cytotoxic T lymphocytes (CTLs) by this type of vaccine (Wiktor et al, 1977;Morgeaux et al, 1989;Sugamata et al, 1990), which are not usually primed by non-replicating vaccines. Here we demonstrate that each of the cytokines tested enhance the antiviral immunity induced by an inactivated rabies vaccine to various degrees.…”

Development of Alternative Approaches for In-Process Quality Control of Rabies Vaccine

“…It has been indicated that in addition to inactivation of virus, BPL also reduces/eliminates the carcinogenic property of BHK-21 cells by strand break and nick of cellular DNA. The damage to the DNA structure by BPL modifies the biological properties of the purified cellular supernatant DNA appraised by its ability to serve as the template in vitro for different polymerases (Morgeaux et al, 1993) and at the same time this does not interfere with the antigenic property of virus for induction of protective immune response. During the present investigation the experimental virus was inactivated using 1:4000 dilution of BPL.…”

Stable expression of rabies virus glycoprotein in Chinese hamster ovary cells

“…In addition, site-directed mutagenesis of G protein can be used to delete and create N-linked oligosaccharide acceptor sites, permitting further study of its structure-function relationships. Since antigenic epitopes on the G protein are preserved in transfected ceils, this suggests that comparison of wild-type and mutant G proteins in transfected mammalian cell lines will be useful for studying both humoral and cellular host immune responses to rabies virus (Bunschoten et al, 1989;Celis et al, 1988a;Eager et al, 1989;Morgeaux et al, 1989). In summary, a model system for studying the rabies virus G protein is described which should be useful in a wide range of studies.…”