Evaluation of the Indian TrueNAT micro RT-PCR device with GeneXpert for case detection of pulmonary tuberculosis

Nikam, Chaitali; Kazi, Mubin; Nair, Chandrasekhar Bhaskaran; Jaggannath, Majula; Manoj, Manesh; Vinaya, R. V.; Shetty, Anjali; Rodrigues, Camilla

doi:10.1016/j.ijmyco.2014.04.003

Cited by 61 publications

(42 citation statements)

References 13 publications

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“…In this study, less than half who advised bacteriological diagnosis asked for both rapid and culture tests; some used culture methods alone which, although accurate, are time consuming and delay diagnosis. Use of rapid molecular techniques like Xpert MTB/RIF is useful to prevent delay but it only detects resistance to rifampicin [ 35 , 36 ]. Presence of resistance to other antibiotics goes undetected and it does not help in formulating individualised regimen.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis and Treatment of Childhood Pulmonary Tuberculosis: A Cross-Sectional Study of Practices among Paediatricians in Private Sector, Mumbai

Tauro

Gawde

2015

Interdisciplinary Perspectives on Infectious Diseases

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Majority of children with tuberculosis are treated in private sector in India with no available data on management practices. The study assessed diagnostic and treatment practices related to childhood pulmonary tuberculosis among paediatricians in Mumbai's private sector in comparison with International Standards for Tuberculosis Care (ISTC) 2009. In this cross-sectional study, 64 paediatricians from private sector filled self-administered questionnaires. Cough was reported as a symptom of childhood TB by 77.8% of respondents. 38.1% request sputum smear or culture for diagnosis and fewer (32.8%) use it for patients positive on chest radiographs and 32.8% induce sputum for those unable to produce it. Sputum negative TB suspect is always tested with X-ray or tuberculin skin test. 61.4% prescribe regimen as recommended in ISTC and all monitor progress to treatment clinically. Drug-resistance at beginning of treatment is suspected for child in contact with a drug-resistant patient (67.7%) and with prior history of antitubercular treatment (12.9%). About half of them (48%) request drug-resistance test for rifampicin in case of nonresponse after two to three months of therapy and regimen prescribed by 41.7% for multidrug-resistant TB was as per ISTC. The study highlights inappropriate diagnostic and treatment practices for managing childhood pulmonary TB among paediatricians in private sector.

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Section: Discussionmentioning

confidence: 99%

Diagnosis and Treatment of Childhood Pulmonary Tuberculosis: A Cross-Sectional Study of Practices among Paediatricians in Private Sector, Mumbai

Tauro

Gawde

2015

Interdisciplinary Perspectives on Infectious Diseases

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“…Studies on the Truenat MTB test (Molbio Diagnostics, India) [19] undertaken in India found sensitivity and specificity to be similar to that of the Xpert MTB/RIF assay [20,21], whereas a study using EasyNAT in Tanzania reported high specificity (100%) and a sensitivity compared with culture of 66.7%, with 10% of smear-negative cases found to be positive [22]. A study from China on the EasyNAT Diagnostic Kit (Ustar Biotechnologies, China) [23] using processed sputum reported sensitivity and specificity of 84% and 98%, respectively.…”

Section: Nucleic Acid Amplification Testsmentioning

confidence: 99%

Perspectives on Advances in Tuberculosis Diagnostics, Drugs, and Vaccines

et al. 2015

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Despite concerted efforts over the past 2 decades at developing new diagnostics, drugs, and vaccines with expanding pipelines, tuberculosis remains a global emergency. Several novel diagnostic technologies show promise of better point-of-care rapid tests for tuberculosis including nucleic acid-based amplification tests, imaging, and breath analysis of volatile organic compounds. Advances in new and repurposed drugs for use in multidrug-resistant (MDR) or extensively drug-resistant (XDR) tuberculosis have focused on development of several new drug regimens and their evaluation in clinical trials and now influence World Health Organization guidelines. Since the failure of the MVA85A vaccine 2 years ago, there have been no new tuberculosis vaccine candidates entering clinical testing. The current status quo of the lengthy treatment duration and poor treatment outcomes associated with MDR/XDR tuberculosis and with comorbidity of tuberculosis with human immunodeficiency virus and noncommunicable diseases is unacceptable. New innovations and political and funder commitment for early rapid diagnosis, shortening duration of therapy, improving treatment outcomes, and prevention are urgently required.

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“…However, it has poor sensitivity (~70%) and is dependent on the skill of the personnel performing the test. 7,10 If the result of the AFB staining is negative but the clinical suspicion is high, culturing should be performed to test for M. tuberculosis. 4,22 Culturing is a golden standard in the diagnosis of M. tuberculosis infection.…”

Section: Diagnosis: Methods and Samplesmentioning

confidence: 99%

“…The assay uses quantitative real-time polymerase chain reaction (qRT-PCR) to detect both M. tuberculosis and rifampicin resistance within 2 hours. [5][6][7] This review discusses the basic concept of TB, the different types of diagnostic methods, and the specimens for TB diagnosis.…”

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confidence: 99%

A recent update of the diagnostic methods for tuberculosis and their applicability in Indonesia: a narrative review

Susilawati

Larasati

2019

Med J Indones

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Tuberculosis (TB) is a major global problem, especially with the high prevalence of HIV-TB co-infection. Delayed diagnosis and continual transmission contribute to high mortality in Indonesia, which has the third highest incidence of TB in the world, after China and India. Therefore, early diagnosis is needed to reduce the number of cases and to administer therapy to prevent the transmission of bacteria. The diagnosis of TB remains a challenge in clinical practice due to poor sensitivity and the requirement of skilled staff in microscopic tests, the slow growth Mycobacterium in culture, and the low number of bacilli present in extrapulmonary TB. Despite being the golden standard for TB diagnosis, cultures require 2–8 weeks to grow. Other methods for diagnosing TB include interferon-gamma release assays and serologic tests such as the tuberculin skin test. Recently, the World Health Organization recommended the GeneXpert MTB/RIF assay for diagnosing TB. This review presents the current state of TB epidemiology and various methods for TB diagnosis. In particular, the paper provides an in-depth discussion about the GeneXpert MTB/RIF assay that has been made available recently in selected tertiary hospitals in Indonesia.

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Evaluation of the Indian TrueNAT micro RT-PCR device with GeneXpert for case detection of pulmonary tuberculosis

Cited by 61 publications

References 13 publications

Diagnosis and Treatment of Childhood Pulmonary Tuberculosis: A Cross-Sectional Study of Practices among Paediatricians in Private Sector, Mumbai

Diagnosis and Treatment of Childhood Pulmonary Tuberculosis: A Cross-Sectional Study of Practices among Paediatricians in Private Sector, Mumbai

Perspectives on Advances in Tuberculosis Diagnostics, Drugs, and Vaccines

A recent update of the diagnostic methods for tuberculosis and their applicability in Indonesia: a narrative review

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