Evaluation of the in vivo leishmanicidal activity of amphotericin B emulgel: An alternative for the treatment of skin leishmaniasis

Pinheiro, Iluska Martins; Carvalho, Ivana Pereira Santos; Carvalho, Chrislanne Barreira de Macêdo; Brito, Lucas Moreira; Silva, Andrezza Braga Soares da; Júnior, Aírton Mendes Conde; Carvalho, Fernando Aécio de Amorim; Carvalho, André Luís Menezes

doi:10.1016/j.exppara.2016.02.010

Cited by 23 publications

(8 citation statements)

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“…Recent reports described formulations based on PL F-127 micelles encapsulating a naphthoquinone derivative (Mendonça et al, 2019), clioquinol (Tavares et al, 2019), amphotericin B (Mendonça et al, 2016), and PL F-68 micellar systems for amphotericin B (Espuelas et al, 2000). In this study, we present the development of lipid-PL formulations containing CUR; in particular, OA has been described as an important component of skin formulations for enhanced efficacy on leishmaniosis treatment (Pinheiro et al, 2016), as well as due to its involvement on transition from promastigotes to amastigotes (Bouazizi-Ben et al, 2017). In this context, further experiments will be necessary in order to evaluate the performance of each formulation and its isolated components on proliferation process for both promastigote and amastigote host-cell stages as well as on different Leishmania strains.…”

Section: Discussionmentioning

confidence: 99%

Physico-Chemical Characterization and Biopharmaceutical Evaluation of Lipid-Poloxamer-Based Organogels for Curcumin Skin Delivery

et al. 2019

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Organogels (ORGs) are semi-solid materials, in which an organic phase is immobilized by a three-dimensional network composed of self-organized system, forming the aqueous phase. In this context, lipid–Pluronics (PLs) ORGs form a two-phase system which can be effectively used as skin delivery systems, favoring their permeation across the skin. In this study, we presented the development of ORG skin drug-delivery systems for curcumin (CUR), a liposoluble phenolic pigment extracted from the turmeric rhizome. In special, we designed the formulation compositions in order to carry high amounts of CUR soluble in oleic acid (OA), as organic phase, entrapped into an aqueous phase composed of micellar PL-based hydrogels by associating two polymers with different hydrophilic–lipophilic balances, Pluronic F-127 (PL F-127), and Pluronic L-81 (PL L-81), to enhance the permeation across the skin. Results revealed that the incorporation of PL L-81 favored the CUR incorporation into micelle–micelle interface. CUR insertion into OA-PL F-127/L-81 reduced both G’/G” relationship (∼16 x) and viscosity values (η* ∼ 54 mPa.s, at 32.5°C), disturbing the ORG network structural organization. In vitro permeation assays through Strat-M® skin-model membranes showed that higher CUR-permeated amounts were obtained for OA-PL F-127/L-81 (4.83 µg.cm−2) compared to OA-PL F-127 (3.51 μg.cm−2) and OA (2.25 μg.cm−2) or hydrogels (∼1.2 μg.cm−2, p < 0.001). Additionally, ORG formulations presented low cytotoxic effects and evoked pronounced antileishmanial activity (IC50 < 1.25 µg.ml−1), suggesting their potential use as skin delivery systems against Leishmania amazonensis. Results from this study pointed out OA-PL-based ORGs as promising new formulations for possible CUR topical administration.

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Section: Discussionmentioning

confidence: 99%

Physico-Chemical Characterization and Biopharmaceutical Evaluation of Lipid-Poloxamer-Based Organogels for Curcumin Skin Delivery

et al. 2019

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“…There are active compounds with anti-microbial activity such as oleic acid and coumarin in aqueous and alcoholic extracts (29,30). The prenylated coumarins auraptene, galbanic acid and umbelliprenin isolated from another genus Apiaceae (Ferula szowitsiana) were revealed invitro antileishmanial effects on L. major promastigotes in comparison with the negative control (31,32,33).…”

Section: Discussionmentioning

confidence: 99%

“…The suppressive effects of methanol extract against acute and subacute sickness, type 4 allergic reactions through cyclooxygenase and lipoxygenase inhibition have already been confirmed ( 28 ). There are active compounds with anti-microbial activity such as oleic acid and coumarin in aqueous and alcoholic extracts ( 29 , 30 ). The prenylated coumarins auraptene, galbanic acid and umbelliprenin isolated from another genus Apiaceae ( Ferula szowitsiana ) were revealed invitro antileishmanial effects on L. major promastigotes in comparison with the negative control ( 31 , 32 , 33 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of Foeniculum Vulgare Aqueous and Alcoholic Seed Extract against Zoonotic Cutaneous Leishmaniasis

Gholamrezaei¹,

Jalallou²,

javad³

et al. 2021

Ethiop J Health Sci

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BACKGROUND፡ Cutaneous leishmaniasis is considered one of the major neglected tropical diseases. Drug resistance, limitary efficacy, and severe side effects remain a challenge for treatment. Foeniculum vulgare is known as a medicinal plant belonging to the Apiaceae, and anti-microbial properties of this plant have already been confirmed.METHOD: The F.vulgare sterile aqueous and alcoholic extracts were prepared. In vitro has used RAW 264.7 cell line and L. major parasite (MRHO/IR/75/ER). Cytotoxicity assay on macrophages (CC50), cytotoxicity assay on promastigotes (IC50), and cytotoxicity assay on infected macrophages (EC50) were accomplished with both extracts by MTT and light microscopy methods. Four in vivo were allocated in four groups and five BALB/c mice each group. Stationary phase promastigotes were inoculated into the base of mice tails subcutaneously (SC).Measurement of the body weight, lesion size, parasite burden of the lesion, and spleen after 4 weeks for evaluation effects of the alcoholic extract on CL was done.RESULTS: The results of in vitro revealed that the optimal concentrations of both extracts reducing the promastigotes and amastigotes growth. Alcoholic extract no harmful side effects for the host macrophages, while were indicated has a potent action against L. major. In vivo results after 4 weeks did not show any variation in lesion size and body weight. Also, lesion size and spleen parasite burden decreased in comparison to no treatment group.CONCLUSION: The alcoholic extract could be a new alternative treatment for cutaneous leishmaniasis. However this extract needs more investigation for novel herbal drugs against CL.

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“…Although the treatments against these protozoa have acceptable efficacy, some of these diseases have a limited number of therapeutic agents which have considerable side effects. Drugs for diseases such as leishmaniasis and Chagas disease have limitations, such as high cost, significant adverse effects, and low patient compliance [77–79] . One of the main problems with these treatments is resistance, [78,80–82] and, in general, their use of must be accompanied by monitoring to ensure their effectiveness [80–83] …”

Section: Antiparasitic Activities Of Propolismentioning

confidence: 99%

Antiparasitic Properties of Propolis Extracts and Their Compounds

Paula

Cândido

Santos

et al. 2021

Chemistry & Biodiversity

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Propolis is a bee product that has been used in medicine since ancient times. Although its anti‐inflammatory, antioxidant, antimicrobial, antitumor, and immunomodulatory activities have been investigated, its anti‐parasitic properties remain poorly explored, especially regarding helminths. This review surveys the results obtained with propolis around the world against human parasites. Regarding protozoa, studies carried out with the protozoa Trypanosoma spp. and Leishmania spp. have demonstrated promising results in vitro and in vivo. However, there are fewer studies for Plasmodium spp., the etiological agent of malaria and less so for helminths, particularly for Fasciola spp. and Schistosoma spp. Despite the favorable in vitro results with propolis, helminth assays need to be further investigated. However, propolis has shown itself to be an excellent natural product for parasitology, thus opening new paths and approaches in its activity against protozoa and helminths.

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Evaluation of the in vivo leishmanicidal activity of amphotericin B emulgel: An alternative for the treatment of skin leishmaniasis

Cited by 23 publications

References 45 publications

Physico-Chemical Characterization and Biopharmaceutical Evaluation of Lipid-Poloxamer-Based Organogels for Curcumin Skin Delivery

Physico-Chemical Characterization and Biopharmaceutical Evaluation of Lipid-Poloxamer-Based Organogels for Curcumin Skin Delivery

Effect of Foeniculum Vulgare Aqueous and Alcoholic Seed Extract against Zoonotic Cutaneous Leishmaniasis

Antiparasitic Properties of Propolis Extracts and Their Compounds

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