Evaluation of the in vivo antioxidant, toxicological and chromatographical profiling of leaf extract and fractions of Crescentia cujete Linn. (Bignoniaceae)

Anwuchaepe, Amarachukwu Ukamaka; Onyegbule, Felix A.; Ajaghaku, Daniel Lotanna; Nwafor, Felix Ifeanyi; Okoye, Festus Basden Chiedu

doi:10.21276/apjhs.2017.4.3.8

Cited by 6 publications

(13 citation statements)

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“…Again, glycosides, terpenes, and flavonoids have been reportedly detected from its stembark and leaves [40]. In terms of chemical components of extracts of various parts, studies [8,30,34,35,[38][39][40][41][42][43][44][45] have established diverse chemical moieties obtained through either chemical profiling and/or characterization processes.…”

Section: Phytochemistrymentioning

confidence: 99%

“…e acute toxicity profile of the fruit and leaves of the plants were evaluated based on organization for economic corporation and development (OECD) 425 guidelines in the reports of Shastry et al [51] from India and Anwuchaepe et al [8] from Nigeria, respectively. e animals were reported to show no signs of toxicity and mortality at the tested concentrations, indicating that the LD 50 is thus above 2000 and 5000 mg/kg bodyweight, respectively, and, hence, could be considered safe for consumption below 5000 mg/kg body weight.…”

Section: Wound Healingmentioning

confidence: 99%

“…e genus Crescentia is endowed with seven species including amazonica, linearifolia, latifolia, plectantha, portoricensis, alata, and cujete, with the latter two known for their numerous medicinal properties [2,3]. e plant is, otherwise, known as Crescentia acuminata with numerous local names (depending on cultures, tribes, and country) such as Calabash or Gourd tree (English), Calebassier (France), Kalbas (Afrikaans), Totumo (Bolivia), Boan-gota (Bangladesh), Maja or Bila (Indonesia), Miracle fruit (Philippines), Osisi mkpo or Oba, Igi igba, Uko, Ugbuba, Gumbusi mboro (Nigeria), Cujuba, Cuieira, Cabaça (Brazil), Labu kayu (Malaysia), Hu lu shu (China), Tanpura (Bengali), Jicaro tree (Honduras), and Higueron (Peru) [3][4][5][6][7][8][9][10]. e mode of preparations and the use of the plant for medicinal purposes vary with tribe or cultures.…”

Section: Introductionmentioning

confidence: 99%

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A Review of the Phytochemistry, Ethnobotany, Toxicology, and Pharmacological Potentials of Crescentia cujete L. (Bignoniaceae)

Balogun

Sabiu

2021

Evidence-Based Complementary and Alternative Medicine

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Crescentia cujete is an economical and medicinal plant of wide indigenous uses including hypertension, diarrhea, respiratory ailments, stomach troubles, infertility problems, cancer, and snakebite. Despite these attributes, C. cujete is largely underutilized, notwithstanding the few progresses made to date. Here, we reviewed the available findings on the ethnobotany, phytochemistry, toxicology, and pharmacology, as well as other economic benefits of the plant. The information on the review was gathered from major scientific databases (Google scholar, Scopus, Science Direct, Web of Science, PubMed, Springer, and BioMed Central) using journals, books, and/or chapters, dissertations, and conference proceedings. The review established the antidiabetic, antioxidant, acaricidal, antibacterial, anti-inflammatory, anthelmintic, antivenom, wound healing, neuroprotection, antiangiogenic, and cytotoxic properties from aqueous and organic (particularly ethanol) aerial parts attributed to several secondary metabolites such as flavonoids, alkaloids, saponins, tannins, phenols, cardiac glycosides, phytosterols, reducing sugar, and volatile oils. Economically, the fruit hard outer shell found applications as musical tools, tobacco pipes, bowls, food containers, and bioethanol production. While most of the current studies on C. cujete are mainly from Asia and South America (Philippines, Bangladesh, India, etc.), part of the persistence challenge is lack of comprehensive data on the plant from in vivo pharmacological studies of its already characterized compounds for probable clinical trials toward drug discovery. Consequently, upon this, modern and novel translational studies including the concept of ‘-omics’ are suggested for studies aiming to outfit more comprehensive data on its therapeutic profiles against pathological markers of diseases and to fully explore its economic benefits.

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Section: Phytochemistrymentioning

confidence: 99%

Section: Wound Healingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Review of the Phytochemistry, Ethnobotany, Toxicology, and Pharmacological Potentials of Crescentia cujete L. (Bignoniaceae)

Balogun

Sabiu

2021

Evidence-Based Complementary and Alternative Medicine

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“…Enniatin B is a secondary compound with ionphoric characteristics; it is produced by the fungi Fusarium and can serve as herbicidal, antifungal, insecticidal, and antibacterial compound. Following the report by Tsukamoto et al, [29] dipiperamides E has an inhibitory effect on drug metabolizing enzyme cytochrome P450 (CYP) 3AA while Isoprunetin can serve as an antioxidant [30,31]. According to Prosperini et al, [32] Antosiak et al [33].…”

Section: Hplc-dad Analysismentioning

confidence: 98%

Antimicrobial Evaluation of the Extract/Fractions of the Millettia aboensis (Leguminosae) Stem against Streptococcus mutans

Ajaegbu¹,

Ezugworie²,

Dieke³

et al. 2020

EJMP

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Aims: Millettia aboensis (Hook.f.) Baker belongs to the Leguminosae family, known locally as nduezi in Igbo, erurumesi in Edo, and Òdúdū in Efik. Millettia aboensis stem is a rich source of flavonoids, phenolic acid, alkaloids, and steroids, hence with medicinal and physiological potentials. It is used in traditional medicine for general healing of diseases including ulcers and laxatives. The present study was aimed at evaluating the antibacterial potential of the extract/fractions of the stem of M. aboensis against Streptococcus mutans – dental caries causative organism and detection of its principles. Methodology: Cold maceration in methanol and liquid-liquid fractionation techniques using hexane, ethyl acetate, and butanol as solvents were utilized for the extraction and fractionation processes respectively. Some phytochemicals from the fractions were suspected using High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD). The crude methanol stem extract/fractions were screened to analyze its antibacterial activity against Streptococcus mutans. Results: The results showed for the fractions that aqueous fraction extract had the highest percentage yield (57.73%), followed by ethyl acetate (16.79%), butanol (12.17%), hexane (9.48%), while the percentage yield of the methanol extract is 2.98%. The HPLC-DAD analysis detected the following phytochemical constituents from the stem fractions: pestalotioprolide C - 1, corynesidone D - 2, enniatin B - 3, dipiperamide E - 4, isopranetin 8-C-glucoside - 5, genistein 8-C-glucoside - 6, genistein 6-C-glucoside - 7, and peniciaculin B - 8. In relatively low and high concentrations (6.25 – 50 mg/ml), the extract/fractions of M. aboensis were found ineffective against Streptococcus mutans. Conclusion: The plant – M. aboensis with its phytochemicals present could be an excellent source of novel biologically active compounds with pharmaceutical and industrial importance.

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“…In the human body, antioxidant compounds can maintain optimal health. Antioxidants function as anti-carcinogenic, regenerate cells, and anti-mutagenic 16 . Research on plants and fruits shows strong antioxidants activity such as flavonoids, phenolics, and tannins compounds are important bioactive which are efficacious for protection from disease.…”

Section: Introductionmentioning

confidence: 99%

Total Phenolic and Flavonoid Content, Antioxidant, and Toxicity Test with BSLT of Meistera chinensis Fruit Fraction from Southeast Sulawesi

et al. 2021

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Meistera chinensis is one of the new generations of the Zingiberaceae family. Zingiberaceae have a large number of species and still need research to reveal their chemical content. Meistera chinensis, a local plant that is widespread in Konawe Regency, Southeast Sulawesi. Empirically, M. chinensis is used as a flavor enhancer in food, aches, and increases body immunity. There are no reports of chemical content or its biological activity. In this study, the fruit was tested for phytochemicals, antioxidants using the ABTS test, total phenolic, total flavonoid content, and toxicity test with BSLT. Meistera chinensis was extracted with ethanol and fractionated by ethyl acetate solvent use vacuum liquid chromatography. Phytochemical screening was carried out qualitatively by using the calorimetric method. The fraction toxicity was monitored by a lethal test for brine shrimp (BSLT). These fractions for the ABTS method obtained fractions 1-8 (F1-F8) and ascorbic acid were used as controls. The results showed radical scavenging activities fraction of M. chinensis fruit was a very strong activity with IC50 of 42.7±3.53 mg/L (F8). The total phenolic and flavonoid contents were 30.72±1.07 mgGAE/g and 8.02±0.48 mgQE/g, respectively. The phytochemical evaluation contains terpenoids, saponins, phenolics, steroids, alkaloids, and flavonoids. The BSLT toxicity test was found to be very toxic with IC50 of 5.20±0.72 mg/L. These findings indicate that the fruit of M. chinensis acts as an antioxidant and toxicity agent.

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Evaluation of the in vivo antioxidant, toxicological and chromatographical profiling of leaf extract and fractions of Crescentia cujete Linn. (Bignoniaceae)

Cited by 6 publications

References 0 publications

A Review of the Phytochemistry, Ethnobotany, Toxicology, and Pharmacological Potentials of Crescentia cujete L. (Bignoniaceae)

A Review of the Phytochemistry, Ethnobotany, Toxicology, and Pharmacological Potentials of Crescentia cujete L. (Bignoniaceae)

Antimicrobial Evaluation of the Extract/Fractions of the Millettia aboensis (Leguminosae) Stem against Streptococcus mutans

Total Phenolic and Flavonoid Content, Antioxidant, and Toxicity Test with BSLT of Meistera chinensis Fruit Fraction from Southeast Sulawesi

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