Evaluation of the in vitro biocompatibility of various elastomers

Chauvel‐Lebret, Dominique; Pellen‐Mussi, Pascal; Auroy, Pascal; Bonnaure‐Mallet, Martine

doi:10.1016/s0142-9612(98)00181-1

Cited by 37 publications

(10 citation statements)

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“…However, these byproducts decrease in cytotoxicity after 1 hour and have a very low cytotoxic effect on intraoral soft tissues [29]. The safety biocompatibility of nonlatex elastics has been well proved through the use of mouth guards in dentistry [30]. In this study, all elastomeric ligatures evaluated showed cell viability, over of 70% in all experimental periods, that allowing affirm the high viability of the evaluated materials.…”

Section: Discussionsupporting

confidence: 50%

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

et al. 2015

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The aim of this study is to evaluate cytotoxicity between polyurethane and non-latex orthodontic elastomeric ligatures. Seven elastomeric ligatures of black-coloured from different manufactures (3 non-latex, 3 polyurethane and 1 latex) were divided into 7 groups of 10 elastics each: Group UK (nonlatex, 3M Unitek), Group TP (nonlatex, TP Orthodontics), Group AO (nonlatex, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident) and Group TC (latexControl, TP Orthodontics). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with 1-way analysis followed by the Tukey post hoc test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05). There was a statistically differences (p<.05) between the viability cell of Groups UK, TP and AO with the Groups O, M, TD and TC at 1, 2, 3 and 7 days. There was no statistically differences (p>.05) between the groups O, M and TD at in all experimental times. It can be concluded that the nonlatex elastomeric ligatures of Unitek, TP Orthodontics, and American Orthodontics showed higher cell viability compared to others ligatures.

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Section: Discussionsupporting

confidence: 50%

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

et al. 2015

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“…Zohar et al [1998a] showed that osteopontin is expressed early during osteogenesis and at different levels during the maturation of osteoblastic cells. Flow cytometry is a useful procedure for measuring in vitro the biocompatibility of implant materials for orthopedic and dental surgery [Chauvel-Lebret et al, 1999;Lopes et al, 1998;Prigent et al, 1998;Sumner et al, 1991;Wilke et al, 1998;Zohar et al, 1998a]. In this study, osteoblasts grown on collagen-coated titanium alloy were found to express more osteopontin when compared with the uncoated surface.…”

Section: Osteoblastic Differentiationmentioning

confidence: 81%

Synergistic Effect of Titanium Alloy and Collagen Type I on Cell Adhesion, Proliferation and Differentiation of Osteoblast-Like Cells

Roehlecke

Witt²,

Kasper³

et al. 2001

Cells Tissues Organs

112

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A number of studies have demonstrated the pivotal role of collagen in modulating cell growth and differentiation. In bone, where the extracellular matrix is composed of approximately 85% type I collagen, cellular interaction with matrix components has been shown to be important in the regulation of the osteoblast phenotype. Preservation or enhancement of normal osteoblast function and appositional bone formation after implant placement represents a strategy that can be useful for the purpose of improving osseointegration. In order to further improve biocompatibility, we combined two known favorable compounds, namely the titanium alloy, Ti6A14V, with type I collagen. We assessed the in vitro behavior of primary osteoblasts grown on both fibrillar collagen-coated and tropocollagen-coated Ti6A14V in comparison with uncoated titanium alloy, using an improved adsorption procedure. As parameters of biocompatibility, a variety of processes, including cell attachment, spreading, cytoskeletal organization, focal contact formation, proliferation and expression of a differentiated phenotype, were investigated. Our results demonstrated for the first time that in comparison to uncoated titanium alloy, collagen-coated alloy enhanced spreading and resulted in a more rapid formation of focal adhesions and their associated stress fibers. Growing on collagen-coated Ti6A14V, osteoblasts had a higher proliferative capacity and the intracellular expression of osteopontin was upregulated compared to uncoated titanium alloy. Type I collagen-coated titanium alloy exhibits favorable effects on the initial adhesion and growth activities of osteoblasts, which is encouraging for its potential use as bone graft material. Moreover, collagen type I may serve as an excellent biocompatible carrier for osteotropic factors such as cell adhesion molecules (e.g. fibronectin) or bone-specific growth factors.

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“…PS and PAA were chosen as components of our swellable MN system given their established potential to exhibit only a minimal inflammatory response upon implantation 34, 35 . While we envision the approach could be employed with biodegradable materials, diffusion based drug delivery through the swollen non-degradable MN is controllable and likely useful for short-term applications.…”

Section: Discussionmentioning

confidence: 99%

A bio-inspired swellable microneedle adhesive for mechanical interlocking with tissue

et al. 2013

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Achieving significant adhesion to soft tissues while minimizing tissue damage poses a considerable clinical challenge. Chemical-based adhesives require tissue-specific reactive chemistry, typically inducing a significant inflammatory response. Staples are fraught with limitations including high-localized tissue stress and increased risk of infection, and nerve and blood vessel damage. Here, inspired by the endoparasite Pomphorhynchus laevis which swells its proboscis to attach to its host’s intestinal wall, we have developed a biphasic microneedle array that mechanically interlocks with tissue through swellable microneedle tips, achieving ~ 3.5 fold increase in adhesion strength compared to staples in skin graft fixation, and removal force of ~ 4.5 N/cm2 from intestinal mucosal tissue. Comprising a poly(styrene)-block-poly(acrylic acid) swellable tip and non-swellable polystyrene core, conical microneedles penetrate tissue with minimal insertion force and depth, yet high adhesion strength in their swollen state. Uniquely, this design provides universal soft tissue adhesion with minimal damage, less traumatic removal, reduced risk of infection and delivery of bioactive therapeutics.

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Evaluation of the in vitro biocompatibility of various elastomers

Cited by 37 publications

References 0 publications

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

Synergistic Effect of Titanium Alloy and Collagen Type I on Cell Adhesion, Proliferation and Differentiation of Osteoblast-Like Cells

A bio-inspired swellable microneedle adhesive for mechanical interlocking with tissue

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