Evaluation of the In Vitro Antimicrobial Activity of Ethanolic Extracts Derived from Leaves Of Camellia Japonica Cultivars (Theaceae) Against Enterobacter Cloacae Strain

Kharchenko, Igor; Tkachenko, Halyna; Buyun, Lyudmyla; Kurhaluk, Natalia; Góralczyk, Anna; Maryniuk, Myroslava; Tomin, Władimir; Osadowski, Zbigniew

doi:10.15414/agrobiodiversity.2019.2585-8246.333-347

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…The ethanolic extracts obtained from leaves and pseudobulbs of five Coelogyne spp. were found to exhibit fairly strong antibacterial activity towards Enterobacter cloacae strain used, the diameter of inhibition zones varied within 8.0-25.5 mm (Buyun et al, 2019).…”

Section: Resultsmentioning

confidence: 95%

Evaluation of Antibacterial Activity of the Ethanolic Extracts Derived from Leaves of Coelogyne brachyptera Rchb. f. (Orchidaceae)

2021

Agrobiodivers Improv Nutr health life Qual

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The present study was aimed to assess the antibacterial activity of ethanolic extract obtained from leaves of Coelogyne brachyptera Rchb. f. For the current study, а panel of organisms including Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 25923™) (mecA negative), S. aureus subsp. aureus Rosenbach (ATCC® 29213™) (mecA negative, Oxacillin sensitive, weak β-lactamase producing strain), S. aureus NCTC 12493 (mecA positive, Methicillin-resistant, EUCAST QC strain for cefoxitin), Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™), E. coli (Migula) Castellani and Chalmers (ATCC® 35218™), Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 27583™) were used. The antimicrobial susceptibility testing was done on Muller-Hinton agar by the disc diffusion method. The results of our study revealed the differential efficacy of ethanolic extract obtained from leaves of C. brachyptera on the test organisms. The ethanolic extract obtained from leaves of C. brachyptera revealed significant antibacterial activity against studied strains compared to control samples (96 % ethanol). A statistically significant increase (p <0.05) in the inhibition zone diameters of strain growth was 47 % for S. aureus subsp. aureus ATCC® 25923™, 40 % for S. aureus subsp. aureus ATCC® 29213™, and 44 % for S. aureus NCTC 12493. A non-significantly increase in inhibition zone diameters of E. coli strains' growth was also observed. The plant can be a source material to the herbal drug industry since it has some important antimicrobial components in the extracts that can be used for the development of therapeutic phytomedicine and phytoveterinary.

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Section: Resultsmentioning

confidence: 95%

Evaluation of Antibacterial Activity of the Ethanolic Extracts Derived from Leaves of Coelogyne brachyptera Rchb. f. (Orchidaceae)

2021

Agrobiodivers Improv Nutr health life Qual

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“…Wilson, La Pace, Mrs. Lyman Clarke, Benikarako, and Fanny Bolis. Among them, the extracts from the variety Mrs. Lyman Clarke caused the highest antimicrobial activity against Enterobacter cloacae ( Kharchenko, 2019 ). Therefore, according to these previous results, it is suggested that C. japonica extracts show a greater effectiveness against Gram-positive bacteria, with only a slight activity reported for Gram-negative bacteria.…”

Section: Bioactive Compounds Isolated From C Japonicamentioning

confidence: 99%

Camellia japonica: A phytochemical perspective and current applications facing its industrial exploitation

et al. 2022

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“…In our previous study, the in vitro antimicrobial activity of ethanolic extracts of leaves derived from Camellia japonica (cultivars Kramer's Supreme, C.M. Wilson, La Pace, Mrs. Lyman Clarke, Benikarako, Fanny Bolis) against clinical cefuroxime-resistant Enterobacter cloacae strain was evaluated (Kharchenko et al, 2019). It was revealed that C. japonica and its cultivars possess a mild antibacterial efficacy.…”

Section: Synergistic Antimicrobial Activity Of C Sinensis Andmentioning

confidence: 99%

Antimicrobial Efficacy of Ethanolic Extracts Obtained from Leaves of Camellia japonica L. Cultivars against Escherichia coli Strain

2021

Agrobiodivers Improv Nutr health life Qual

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The present study was aimed to determine the antibacterial activity of six plants, i.e. Camellia japonica L. (cultivars Kramer's Supreme, C.M. Wilson, La Pace, Mrs. Lyman Clarke, Benikarako, Fanny Bolis) against Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) strain. Ethanolic extracts were prepared by freshly crushed leaves and evaluated for their antimicrobial activity against Escherichia coli ATCC® 25922™ strain using disc diffusion assay. The increase of the mean of the diameters of the inhibition zone was 58.4 % for cv. Kramer's Supreme, 29.2 % for cv. La Pace and cv. Mrs. Lyman Clarke, 22.5 % for cv. Fanny Bolis, 19.1% for cv. Benikarako, and 18 % for cv. C.M. Wilson compared to the control samples (96 % ethanol). Among the six plant extracts, C. japonica 'Kramer's Supreme' exhibited the highest inhibitory zones against the tested strain (the mean of the zone of inhibitions was 14.1 ±1.1 mm). The intermediate activity was presented by other cultivars studied. The findings reported herein give scientific credence to the traditional uses of these plants and suggest that extracts derived from the leaves of Camellia japonica and its cultivars merit further chemical study as natural antibiotics to identify the secondary metabolites. These results could provide a theoretical basis for making full use of Camellia japonica and its cultivars. Moreover, their antibacterial activities can play an important role in medicine, veterinary, food preservation, and other aspects. Mechanisms of antibacterial activities remain to be studied.

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Evaluation of the In Vitro Antimicrobial Activity of Ethanolic Extracts Derived from Leaves Of Camellia Japonica Cultivars (Theaceae) Against Enterobacter Cloacae Strain

Abstract: AGROBIODIVERSITY f o r i m p r o v i n g n u t r i t i o n , h e a l t h a n d l i f e q u a l i t y 2019-333

Cited by 4 publications

References 39 publications

Evaluation of Antibacterial Activity of the Ethanolic Extracts Derived from Leaves of Coelogyne brachyptera Rchb. f. (Orchidaceae)

Evaluation of Antibacterial Activity of the Ethanolic Extracts Derived from Leaves of Coelogyne brachyptera Rchb. f. (Orchidaceae)

Camellia japonica: A phytochemical perspective and current applications facing its industrial exploitation

Antimicrobial Efficacy of Ethanolic Extracts Obtained from Leaves of Camellia japonica L. Cultivars against Escherichia coli Strain

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